Localization of neurons expressing choline acetyltransferase, serotonin and/or FMRFamide in the central nervous system of the decapod shore crab Hemigrapsus sanguineus

Kotsyuba, E. P.; Dyachuk, Vyacheslav

doi:10.1007/s00441-020-03309-3

Cited by 6 publications

(5 citation statements)

References 113 publications

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“…Only a few examples describe antisera staining for ChAT in crustaceans, for example, in the barnacle Balanus amphitrite (Gallus et al., 2006), and in the nervous system of Hemigrapsus sanguineus (Kotsyuba & Dyachuk, 2021) and Paralithodes camschaticus (Kotsyuba & Dyachuk, 2022). However, ChAT staining is well established in D. melanogaster (Seki et al., 2010; Shang et al., 2007; Yasuyama et al., 1995) and other insects like Manduca sexta (Clark et al., 2005; Torkkeli et al., 2005) and Periplanta americana (Fusca et al., 2015).…”

Section: Methodsmentioning

confidence: 99%

Neurochemical diversity in the central olfactory pathway of the crustacean Parhyale hawaiensis (Amphipoda): evolutionary implications

Kümmerlen

Raspe

Harzsch

2023

J of Comparative Neurology

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In mandibulate arthropods, the primary olfactory centers, termed olfactory lobes in crustaceans, are typically organized in distinct fields of dense synaptic neuropils called olfactory glomeruli. In addition to olfactory sensory neuron terminals and their postsynaptic efferents, the glomeruli are innervated by diverse neurochemically distinctive interneurons. The functional morphology of the olfactory glomeruli is understudied in crustaceans compared with insects and even less well understood and described in a particular crustacean subgroup, the Peracarida, which embrace, for example, Amphipoda and Isopoda. Using immunohistochemistry combined with confocal laser scanning microscopy, we analyzed the neurochemistry of the olfactory pathway in the amphipod Parhyale hawaiensis. We localized the biogenic amines serotonin and histamine as well as the neuropeptides RFamide, allatostatin, orcokinin, and SIFamide.As for other classical neurotransmitters, we stained for γ-aminobutyric acid and glutamate decarboxylase and used choline acetyltransferase as indicator for acetylcholine.Our study is another step in understanding principles of olfactory processing in crustaceans and can serve as a basis for understanding evolutionary transformations of crustacean olfactory systems.

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Section: Methodsmentioning

confidence: 99%

Neurochemical diversity in the central olfactory pathway of the crustacean Parhyale hawaiensis (Amphipoda): evolutionary implications

Kümmerlen

Raspe

Harzsch

2023

J of Comparative Neurology

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“…The concurrent use of multiple primary antibodies or proximity ligation assays increases detection specificity for M 3 R [72]. Other immunostaining strategies, such as those targeting ChAT, VAChT, or the choline transporter, may be appropriate if the goal is to describe the distribution of cholinergic components across a tissue that is thousands or tens of thousands of microns in length [73][74][75]. However, the expression of the machinery for ACh synthesis in a particular cell does not necessarily imply increased cholinergic signaling.…”

Section: Current Challenges and Technical Advances In The Study Of Ch...mentioning

confidence: 99%

Cholinergic Mechanisms in Gastrointestinal Neoplasia

Sampaio Moura,

Schledwitz,

Alizadeh

et al. 2024

IJMS

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Acetylcholine-activated receptors are divided broadly into two major structurally distinct classes: ligand-gated ion channel nicotinic and G-protein-coupled muscarinic receptors. Each class encompasses several structurally related receptor subtypes with distinct patterns of tissue expression and post-receptor signal transduction mechanisms. The activation of both nicotinic and muscarinic cholinergic receptors has been associated with the induction and progression of gastrointestinal neoplasia. Herein, after briefly reviewing the classification of acetylcholine-activated receptors and the role that nicotinic and muscarinic cholinergic signaling plays in normal digestive function, we consider the mechanics of acetylcholine synthesis and release by neuronal and non-neuronal cells in the gastrointestinal microenvironment, and current methodology and challenges in measuring serum and tissue acetylcholine levels accurately. Then, we critically evaluate the evidence that constitutive and ligand-induced activation of acetylcholine-activated receptors plays a role in promoting gastrointestinal neoplasia. We focus primarily on adenocarcinomas of the stomach, pancreas, and colon, because these cancers are particularly common worldwide and, when diagnosed at an advanced stage, are associated with very high rates of morbidity and mortality. Throughout this comprehensive review, we concentrate on identifying novel ways to leverage these observations for prognostic and therapeutic purposes.

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“…No. AB143; Millipore, Burlington, MA, United States) was diluted to 1:500 in blocking buffer, and we followed a recently published protocol for ChAT immunostaining (Kotsyuba and Dyachuk, 2021). To avoid nonspecific immunorecognition, we performed immunohistochemistry by bypassing the use of primary antibodies and used only secondary antibodies (1:500-1000; I5006, I5381, and I5256; Sigma, St. Louis, MO, United States).…”

Section: Primary Antibody Specificity and Immunohistochemical Controlmentioning

confidence: 99%

Immunocytochemical Localization of Enzymes Involved in Dopamine, Serotonin, and Acetylcholine Synthesis in the Optic Neuropils and Neuroendocrine System of Eyestalks of Paralithodes camtschaticus

Kotsyuba

Dyachuk

2022

Front. Neuroanat.

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Identifying the neurotransmitters secreted by specific neurons in crustacean eyestalks is crucial to understanding their physiological roles. Here, we combined immunocytochemistry with confocal microscopy and identified the neurotransmitters dopamine (DA), serotonin (5-HT), and acetylcholine (ACh) in the optic neuropils and X-organ sinus gland (XO-SG) complex of the eyestalks of Paralithodes camtschaticus (red king crab). The distribution of Ach neurons was studied by choline acetyltransferase (ChAT) immunohistochemistry and compared with that of DA neurons examined in the same or adjacent sections by tyrosine hydroxylase (TH) immunohistochemistry. We detected 5-HT, TH, and ChAT in columnar, amacrine, and tangential neurons in the optic neuropils and established the presence of immunoreactive fibers and neurons in the terminal medulla in the XO region of the lateral protocerebrum. Additionally, we detected ChAT and 5-HT in the endogenous cells of the SG of P. camtschaticus for the first time. Furthermore, localization of 5-HT- and ChAT-positive cells in the SG indicated that these neurotransmitters locally modulate the secretion of neurohormones that are synthesized in the XO. These findings establish the presence of several neurotransmitters in the XO-SG complex of P. camtschaticus.

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Localization of neurons expressing choline acetyltransferase, serotonin and/or FMRFamide in the central nervous system of the decapod shore crab Hemigrapsus sanguineus

Cited by 6 publications

References 113 publications

Neurochemical diversity in the central olfactory pathway of the crustacean Parhyale hawaiensis (Amphipoda): evolutionary implications

Neurochemical diversity in the central olfactory pathway of the crustacean Parhyale hawaiensis (Amphipoda): evolutionary implications

Cholinergic Mechanisms in Gastrointestinal Neoplasia

Immunocytochemical Localization of Enzymes Involved in Dopamine, Serotonin, and Acetylcholine Synthesis in the Optic Neuropils and Neuroendocrine System of Eyestalks of Paralithodes camtschaticus

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