Localization of NORs in karyotypes of four Rana species

Abstract

NORs were localized by Ag-AS-staining in karyotypes of Rana nigromaculata, R. macrocnemis, R. latastei (2n = 26) and R. chensinensis, 2n = 24. As in the majority of other Rana species, the NORs are located in the long arms of the 10th (or 11 th in R. chensinensis) chromosome pair. The presence of a second secondary constriction in R. latasteL as reported earlier, was not confirmed.

Features of the karyotypes of Pelophylax ridibundus Pallas, 1771 and Rana macrocnemis Boulenger, 1885 (Amphibia: Ranidae) from Armenia

“…Comparison of the chromosomal data of P. ridibundus with other species of the green frogs (Heppich, 1978;Schmid, 1978;Miura, 1995;Miura et al, 1997) and some species of brown frogs (Orlova et al, 1978;Birstein, 1984;Green, Borkin, 1993;Popov, Dimitrov, 1999) with data from this study confi rms the close similarity of several chromosome pairs by size and morphology. On the whole, the interspecifi c differences in karyotypes of species of Rana and Pelophylax are manifested in the distinctions of late replication banding patterns (Miura, 1995;Miura et al, 1997), fl uorescence replication banding patterns (Picariello et al, 2002) and in the locations of C-heterochromatin blocks (Schmid, 1978;Miura, 1995;Miura et al, 1997;present study).…”

“…The comparative analysis of the karyotypes of frogs belonging to the complex of R. macrocnemis from Armenia (our data), Georgia (Ivanov, Madjanov, 1973), (Popov, Dimitrov, 1999) and form "macrocnemis" from Russia (Birstein, 1984;Ivanov, Madjanov, 1973) revealed their similarities on chromosomal pairs № 1, 2, 3, 4, 5, 6, 7 and 12 (Table 4). The cardinal differences of the chromosomal sets of "camerani" and "macrocnemis" forms relate to the chromosomal pairs № 8, 9, 10, 11 and 13 (Table 4).…”

“…Karyotypes of more than 300 studied species of the genera Rana Linnaeus, 1758 and Pelophylax Fitzinger 1883 are characterized by a standard chromosome number of 2n=26 (sometimes 22 or 24) including metacentric, submetacentric and subtelocentric chromosomes (Morescalchi, 1973(Morescalchi, , 1977Ivanov, Madjanov, 1973;Schmid, 1978;Schmid et al, 1990;Orlova et al, 1978;Birstein, 1984;King, 1990;Kuramoto, 1990;Green, Borkin, 1993;Miura, 1995;Alpagut, Falakali, 1995;Spasič-Bošković, 1999;Al-Shehri, Al-Salech, 2005;Suryadnaya, 2003;Kaibeleva et al, 2004;Joshy et al, 2006;Mindrescui, Ghiorghyk, 2008).…”

“…Chromosome sets of brown frogs of the Rana macrocnemis complex have been described from populations from Russia and Georgia (Ivanov, Madyanov, 1973;Orlova et al, 1977;Birstein, 1984;Popov, Dimitrov, 1999). So far chromosome sets of Ranidae species from Armenia have not been studied at all.…”

Comparative cytogenetic analysis of European brown frogs:Rana temporaria, R. dalmatinaandR. graeca

“…Most of the species of the genus Rana share «standard» or «primitive» NOR location -intercalary on the long arm of the lOth chromosome pair (SCHMID 1978b;BIRSTEIN 1984). As in European water frogs, in R. temporaria the NOR presents its standard position.…”