Localization of phospholipase C δ3 in the cell and regulation of its activity by phospholipids and calcium

Pawełczyk, Tadeusz; Matecki, Andrzej

doi:10.1046/j.1432-1327.1998.2570169.x

Cited by 29 publications

(26 citation statements)

References 42 publications

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“…1) deserves some comment because level of PI-PLC d3, which is poorly expressed in normal tissues [Pawelczyk, 1999] but is highly expressed in C6 astrocytoma cells (Figs. 1B and 3) and other neoplastic tissues [Pawelczyk and Matecki, 1998], may be interpreted as a neoplastic upregulation of this enzyme.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, PI-PLC d1 is widely expressed in many tissues, but especially in cultured cells [Tanaka and Kondo, 1994;Adamski et al, 1999;Yamaga et al, 1999;Mao et al, 2000;Faenza et al, 2004]. PI-PLC d3 also has been identified in many histotypes at low concentrations [Pawelczyk and Matecki, 1998]; PI-PLC d4 is expressed in the brain as well as in regenerating tissues [Pawelczyk, 1999]; PI-PLC g1 is expressed in keratinocytes and foetal cartilage [Ananthanarayanan et al, 2002] and has been found almost exclusively in the cytoplasm [McBride et al, 1991;Diakonova et al, 1997]; also PI-PLC e occurs in many tissues [Wing et al, 2003]. Notwithstanding the growing interest for the role played by the PI-PLC family enzymes in the nervous system [Ledeen and Wu, 2004], no definitive studies have been accomplished in glial cells [Choi et al, 1989;Tallant and Higson, 1997;Wang and Reiser, 2003].…”

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confidence: 99%

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Expression of phosphoinositide‐specific phospholipase C isoenzymes in cultured astrocytes

Vasco¹,

Fabrizi²,

Artico³

et al. 2006

J of Cellular Biochemistry

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Signal transduction from plasma membrane to cell nucleus is a complex process depending on various components including lipid signaling molecules, in particular phosphoinositides and their related enzymes, which act at cell periphery and/or plasma membrane as well as at nuclear level. As far as the nervous system may concern the inositol lipid cycle has been hypothesized to be involved in numerous neural as well as glial functions. In this context, however, a precise panel of glial PLC isoforms has not been determined yet. In the present experiments we investigated astrocytic PLC isoforms in astrocytes obtained from foetal primary cultures of rat brain and from an established cultured (C6) rat astrocytoma cell line, two well known cell models for experimental studies on glia. Identification of PLC isoforms was achieved by using a combination of RT-PCR and immunocytochemistry experiments. While in both cell models the most represented PI-PLC isoforms were beta4, gamma1, delta4, and epsilon, isoforms PI-PLC beta2 and delta3 were not detected. Moreover, in primary astrocyte cultures PI-PLC delta3 resulted well expressed in C6 cells but was absent in astrocytes. Immunocytochemistry performed with antibodies against specific PLC isoforms substantially confirmed this pattern of expression both in astrocytes and C6 glioma cells. In particular while some isoenzymes (namely isoforms beta3 and beta4) resulted mainly nuclear, others (isoforms delta4 and epsilon) were preferentially localized at cytoplasmic and plasma membrane level.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Expression of phosphoinositide‐specific phospholipase C isoenzymes in cultured astrocytes

Vasco¹,

Fabrizi²,

Artico³

et al. 2006

J of Cellular Biochemistry

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“…This isoform showed a few notable differences from its sister, PLC␦1, in cellular distribution and sensitivity to lipids and detergents and in the importance of its PH domain in regulation (1202,1203). Recent data indicated that PLC␦3 downregulates RhoA and promotes neurite outgrowth in primary cortical neurons (823).…”

Section: Plc␦3mentioning

confidence: 99%

Phosphoinositides: Tiny Lipids With Giant Impact on Cell Regulation

Balla

2013

Physiological Reviews

1,393

1,655

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Phosphoinositides (PIs) make up only a small fraction of cellular phospholipids, yet they control almost all aspects of a cell's life and death. These lipids gained tremendous research interest as plasma membrane signaling molecules when discovered in the 1970s and 1980s. Research in the last 15 years has added a wide range of biological processes regulated by PIs, turning these lipids into one of the most universal signaling entities in eukaryotic cells. PIs control organelle biology by regulating vesicular trafficking, but they also modulate lipid distribution and metabolism via their close relationship with lipid transfer proteins. PIs regulate ion channels, pumps, and transporters and control both endocytic and exocytic processes. The nuclear phosphoinositides have grown from being an epiphenomenon to a research area of its own. As expected from such pleiotropic regulators, derangements of phosphoinositide metabolism are responsible for a number of human diseases ranging from rare genetic disorders to the most common ones such as cancer, obesity, and diabetes. Moreover, it is increasingly evident that a number of infectious agents hijack the PI regulatory systems of host cells for their intracellular movements, replication, and assembly. As a result, PI converting enzymes began to be noticed by pharmaceutical companies as potential therapeutic targets. This review is an attempt to give an overview of this enormous research field focusing on major developments in diverse areas of basic science linked to cellular physiology and disease.

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“…This gene encodes a protein called phospholipase Cδ3, which is located in the cell membrane and is involved in cell membrane signaling. Control of PLCD3 activity is one of the major starting points of cell regulation (21,22). Therefore, its biological function is involved in the control of the cell cycle by means of the activation of protein kinase C, intracellular storage of Ca +2 ions, signal transduction, and transport through the cell membrane.…”

Section: Discussionmentioning

confidence: 99%

Gene expression analysis reveals novel altered genes in nasal polyps

Babeto

Calmon²,

Provazzi³

et al. 2010

Mol Med Rep

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Abstract. Nasal polyposis (NP) is a chronic inflammatory disease of the nasal mucosa characterized by the infiltration of inflammatory cells, mainly eosinophils. Although nasal polyposis occurs in 4% of the population, its physiopathology remains unclear. The aim of this study was to identify and characterize differentially expressed genes that can be used in the prognosis, treatment and elucidation of this physiopathology. To identify novel genes differentially expressed in NP, we applied real-time quantitative PCR to 11 NP samples and to a pool of total RNA from a subset of 13 normal nasal mucosa samples from human autopsies. For selecting genes, the methylated CpG island amplification technique was used. Five differentially methylated clones (ATP2A1, NOVA1, PLCD3, SOLH and TGFβI) were identified. However, these genes presented methylated CpG islands between exons, i.e., not in the promoter regions of the genes. Thus, as shown by real-time PCR, the ATP2A1, SOLH, PLDC3 and TGFβI genes were overexpressed in NP. The genes identified in this study are probably involved in some stage of the process of formation and development of nasal polyposis, as they were highly expressed in the nasal polyp samples.

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Localization of phospholipase C δ3 in the cell and regulation of its activity by phospholipids and calcium

Cited by 29 publications

References 42 publications

Expression of phosphoinositide‐specific phospholipase C isoenzymes in cultured astrocytes

Expression of phosphoinositide‐specific phospholipase C isoenzymes in cultured astrocytes

Phosphoinositides: Tiny Lipids With Giant Impact on Cell Regulation

Gene expression analysis reveals novel altered genes in nasal polyps

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