Localization of quantitative trait loci (QTL) for agronomic important characters by the use of a RFLP map in barley (Hordeum vulgare L.)

Abstract

Two hundred and fifty doubled haploid lines were studied from a cross between two 2-row winter barley varieties. The lines were evaluated for several characters in a field experiment for 3 years on two locations with two replications. From a total of 431 RFLP probes 50 were found to be polymorphic and subsequently used to construct a linkage map. Quantitative trait loci (QTLs) were determined and localized for resistance against Rhynchosporium secalis and Erysiphe graminis, for lodging, stalk breaking and ear breaking tendency, for the physical state before harvest, plant height, heading date, several kernel parameters and kernel yield. The heritability of the traits ranged from 0.56 to 0.89. For each trait except for kernel thickness, QTLs have been localized that explain 5-52% of the genetic variance. Transgressive segregation occurred for all of the traits studied.

Identification of QTLs for powdery mildew and scald resistance in barley

“…It occurred in the 'Igri' × 'Danilo' cross (Backes et al, 1995), and in the Harrington × TR306 cross (Spaner et al, 1998). In conclusion, all the QTLs for PR against scald detected in the present L94 × 'Vada' cross are supported by previously mapped QTLs in various barley populations.…”

Molecular basis of barley quality

“…Functional gene loci have been found to make a large contribution to an associated QTL (Backes et al 1995;Hayes et al 1993;Li et al 1999aLi et al , 2001Oziel et al 1996). These include Amy1 on chromosome 6H, Bmy1 on chromosome 4H, LD on chromosome 7H, and Hor cluster on chromosome 1H.…”

Fine mapping, physical mapping and development of diagnostic markers for the Rrs2 scald resistance gene in barley

“…spontaneum Rrs12 on 7HS, Rrs13 on 6HS, Rrs14 on 1HS, and Rrs15 on 7HL (Abbott et al 1992;Abbott et al 1995;Garvin et al 1997;Garvin et al 2000;Genger et al 2003a;Genger et al 2003b;Genger et al 2005), or from Hordeum bulbosum Rrs16 on 4HS (Pickering et al 2006). Furthermore, a number of QTL studies revealed QTLs for scald resistance on several chromosomes whose loci often coincided with locations of known scald resistance genes (Backes et al 1995;Bjørnstad et al 2004;Cheong et al 2006;Genger et al 2003;Grønnerød et al 2002;Spaner et al 1998;Thomas et al 1995;von Korff et al 2005;Wagner et al 2008;Yun et al 2005).…”