Localization of Sialyl Glycoconjugates in Eosinophil-Specific Granules after Degranulation Stimuli

Abstract

Sialyl glycoconjugates, a group of the acidic glycoconjugates, are expressed in murine eosinophil-specific granules through their maturational stages. To clarify whether the sialyl glycoconjugates work as functional molecules in the process of eosinophil degranulation, we examined the localization of sialyl residues after degranulation stimulated with calcium ionophore A23187 or interleukin-5. Although sialyl residues were localized in the specific granules before degranulation, they were dissociated from the granules after the stimuli. These results suggest that sialyl glycoconjugates participate extensively in the process of eosinophil degranulation

Carbohydrates Detection in the Hepatic Egg - Granuloma System Using Lectin Histochemistry

“…Studies have observed that carbohydrates of eosinofils surface are involved in the degranulation process (Ohmori & Nawa, 2000), fact which raised attention for the importance of expression and liberation of products from cells in the hepatic granulomas acting as fibrosis inhibitors (van de Vijver et al, 2006). Lectin binding intensity can be influenced by the stage of development of the Schistosoma egg, reflecting the content of carbohydrates in the egg tegument.…”