Localization of substance P, calcitonin gene related peptide and galanin in the nerve fibers of porcine cystic ovaries

Kozłowska, Anna; Wojtkiewicz, Joanna; Majewski, Mariusz; Jana, Barbara

doi:10.5603/fhc.2011.0085

Cited by 10 publications

(7 citation statements)

References 34 publications

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“…The number of galanin nerve fibers contributing to the ground plexus in polycystic ovaries was increased. It appears possible that galanin released from these terminals is relative to the etiopathogenesis of this disorder [85,179]. As aforementioned, the galanin levels are positively associated with obesity [44].…”

Section: The Relationship Between Galanin and Polycystic Ovarian Syndmentioning

confidence: 89%

The regulative effect of galanin family members on link of energy metabolism and reproduction

Fang

Shi

et al. 2015

Peptides

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Section: The Relationship Between Galanin and Polycystic Ovarian Syndmentioning

confidence: 89%

The regulative effect of galanin family members on link of energy metabolism and reproduction

Fang

Shi

et al. 2015

Peptides

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“…Additionally, neural pathways between the ovaries and the CNS arise from, and go to, the hypothalamus and extra-hypothalamic areas [3–7]. The sensory nerves innervating the ovary contain various neurotransmitters, such as substance P (SP), vasoactive intestinal peptide (VIP), and calcitonin gene-related peptide (CGRP) [8–11], galanin [12] and neurokinin A and B [13]. …”

Section: Introductionmentioning

confidence: 99%

In the pubertal rat, the regulation of ovarian function involves the synergic participation of the sensory and sympathetic innervations that arrive at the gonad

Morales‐Ledesma¹,

Hernández

Apolonio³

2015

Reprod Biol Endocrinol

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BackgroundThe present study investigates sectioning the superior ovarian nerve (SON) in rats with functional sensorial denervation induced by capsaicin administration at birth and the effects on the establishment of puberty, ovulation, serum progesterone, and estradiol concentrations.MethodsThe animals were allotted randomly to one of the following experimental groups. Groups of 8–10 rats were injected at birth with capsaicin or vehicle, and on day 20 or 28 of life, they were submitted to a sham operation (SO). Other groups of 8–10 rats were injected at birth with capsaicin or vehicle, and on day 20 or 28 of life, they were submitted to the uni-or bilateral SON sectioning. The animals were killed at the first estrus. Serum concentration of progesterone (ng/ml) and estradiol (pg/ml) were measured using a radioimmunoassay.ResultsAnimals treated with capsaicin and subjected at 20 days of life to the left or bilateral section of SON had a delayed age of vaginal opening. Furthermore, animals with a lack of sensory information and subjected to a SO at 28 days of life had the same delay in the age of vaginal opening. Animals with sensorial innervation intact, subjected to unilateral section of the SON at 20 or 28 days of age, showed diminished ovulation rate and number of ova shed by the denervated ovary. In animals with sensorial denervation, the uni-or bilateral sectioning of the SON did not result in changes in ovulation. Progesterone and estradiol levels were different depending on the age of the animal in which the SON section was performed.ConclusionsBased on the present results, we suggest that sympathetic innervation regulates ovulation and the secretion of steroid hormones and that the sensory fibers modulate the sympathetic innervation action on ovarian functions.

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“…It has been established that the mammalian ovary is supplied by sympathetic and sensory nerve fibers [1]- [3] and enclosed intrinsic neurons [4]- [8]. The sympathetic fibers predominantly release noradrenaline (NA) and vasoactive intestinal peptide (VIP), and the sensory fibers release peptides such as CGRP, substance P (SP) and galanin, among others [9]- [12]. Under physiological conditions, nerve fibers are involved in early follicular development and ovarian steroidogenesis [2] [13]- [15], i.e., NA stimulates androgen and progesterone secretion [16] [17], VIP stimulates estrogen production [18] [19] and CGRP and SP are involved in ovarian vascular fluid [9] [12].…”

Section: Introductionmentioning

confidence: 99%

“…The sympathetic fibers predominantly release noradrenaline (NA) and vasoactive intestinal peptide (VIP), and the sensory fibers release peptides such as CGRP, substance P (SP) and galanin, among others [9]- [12]. Under physiological conditions, nerve fibers are involved in early follicular development and ovarian steroidogenesis [2] [13]- [15], i.e., NA stimulates androgen and progesterone secretion [16] [17], VIP stimulates estrogen production [18] [19] and CGRP and SP are involved in ovarian vascular fluid [9] [12]. Ovarian intrinsic neurons have been studied in several mammals (rats, monkeys and humans) [5] [7] and birds (hen and emu) [18] [19] and modulate steroidogenesis and local vascular fluid [2] [6] [14].…”

Section: Introductionmentioning

confidence: 99%

Morphology and Chemical Phenotype of the Ovarian Intrinsic Neurons in Neonate and Sexually Mature Reproductive Guinea Pig

Luna¹,

Barrientos²,

Alatriste³

et al. 2015

ARSci

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Introduction: The existence of ovarian intrinsic neurons is well established. However, the morphology and chemical phenotype are not completely characterized and are even unknown for some species used in medical research. The purpose of this work was to determine the morphology and chemical phenotype of intrinsic neurons of the guinea pig ovary at two ages: neonates (0 days old) and sexually mature reproductive animals (90 days old). Materials and Methods: For the morphological analysis, we employed the modified Golgi-Cox impregnation technique. For the chemical phenotype, we used immunohistochemistry and the following antibodies; tyrosine hydroxylase (TH), calcitonin gene-related peptide (CGRP), transient receptor potential type 1 (TRPV1), neuron-specific nuclear protein (NeuN) and proto-oncogene product of the cFos gene (cFos). We also used enzyme histochemistry for NADPH-diaphorase detection. Results: The number of intrinsic neurons in the neonate ovary was low in comparison to the adult guinea pig ovary. The intrinsic neurons were located in the cortex and the ovarian medulla; some were isolated or clustered, forming ganglia, and others were interconnected and formed networks. The neurons were small, medium or large. In the cortex of neonate vs adult ovaries, the small and medium neurons comprised 23% vs 36% and 5.2% vs 11.6%, respectively. In the medulla, the percent of the same neurons was 10.1% vs 10.1% and 1.1% vs 2.2% in the neonate and adult, respectively. In both cortex and medulla < 1% were large neurons at two ages. Also, the neurons were rounded, fusiform or multipolar. In the cortex, they were 12.7% vs 20.9%, 14.9% vs 24.2% and 1.1% vs 3.0%, respectively. In the medulla, the percent of small vs medium neurons was 6% vs 7.1% and 4.1% vs 4.8% in the neonate and adult ovary, respectively, and <1% were large neurons at both ages. The chemical phenotypes were in the neonate and adult: TH/NeuN-positive neurons, 16.3% vs 26.5%; CGRP/NeuN, 13.5% vs 35.8%; TRPV1/NeuN, 10.2% vs 38.6%; and cFos/NeuN, 4.6% vs 5.4%, re-* Corresponding author. F. Luna et al. 14 spectively. The percent of NADPHd-positive cells in the cortex was 9.5% vs 25.1% and 3.2% vs 62.2% in the medulla in the neonate and adult, respectively. Conclusion: Altogether, these data showed that the number of ovarian intrinsic neurons was low at birth and increased in the sexually mature reproductive guinea pig. The chemical phenotype was rich and peptidergic, catecholaminergic and nitrergic in nature and positive for cFos immunoreactivity. Therefore, intrinsic neurons can be chemical sensors inside of the gonad and transmit signal to the central nervous system.

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Localization of substance P, calcitonin gene related peptide and galanin in the nerve fibers of porcine cystic ovaries

Cited by 10 publications

References 34 publications

The regulative effect of galanin family members on link of energy metabolism and reproduction

The regulative effect of galanin family members on link of energy metabolism and reproduction

In the pubertal rat, the regulation of ovarian function involves the synergic participation of the sensory and sympathetic innervations that arrive at the gonad

Morphology and Chemical Phenotype of the Ovarian Intrinsic Neurons in Neonate and Sexually Mature Reproductive Guinea Pig

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