Localization of the cytotoxic hydroperoxyeudesmanolides in Artemisia umbelliformis

Cappelletti, E. M.; Caniato, Rosamaria; Appendino, Giovanni

doi:10.1016/0305-1978(86)90059-1

Cited by 50 publications

(31 citation statements)

References 25 publications

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“…Dittmar and Wagner reagents were used as indicators for alkaloids (Furr and Mahlberg 1981) and concentrated sulphuric acid for the detection of terpenoids (Cappelletti et al 1986;Mariani et al 1989). The indicators were applied directly to hand-cut cross-sections of fresh roots on microscope slides and observed with the light microscope.…”

Section: Histochemical Testsmentioning

confidence: 99%

Comparison of cytology and distribution of nickel in roots of Ni-hyperaccumulating and non-hyperaccumulating genotypes of Senecio coronatus

2007

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Two genotypes of Senecio coronatus (Thunb.) Harv. (Asteraceae) growing on ultramaWc outcrops were identiWed previously: a Ni hyperaccumulator and a non-hyperaccumulator. The aim of the present study was to investigate the cytology of the roots of both genotypes, their Ni content and tissue distribution, and to ascertain whether there was a cytological basis for the diVerential uptake of Ni. Light and Xuorescence microscopy together with histochemical methods were used to study root cytology. X-ray microanalysis by means of a nuclear microprobe-particle-induced X-ray emission (PIXE) and proton backscattering (BS) techniques-was utilized to determine the concentration and distribution of Ni and other elements. Average concentration of Ni and distribution in roots diVered signiWcantly between hyperaccumulating and non-hyperaccumulating genotypes. Ni amount in the hyperaccumulating genotype was ca. 60 times higher in the older part of the root (1,760 g g ¡1 ) and ca. 10 times higher (314 g g ¡1 ) in the younger root hair region in comparison with the equivalent parts of the non-accumulating genotype where Ni amounts were 30 g g ¡1 . Ni distribution pattern was also diVerent in both cases. Cytological diVerences were observed in the inner cortical region and exodermis of the roots. Distinct groups of specialized cells with an organelle-rich cytoplasm that produced copious numbers of spherical bodies occurred in the inner cortical region of the hyperaccumulator. Such distinct cell groups were absent from the inner cortex of the non-hyperaccumulator. Cortical cells here had a thin parietal cytoplasmic layer and produced fewer spherical bodies. In both genotypes the spherical structures were extruded from the cytoplasm into air spaces between the cells where they coalesced to form amorphous deposits, signiWcantly larger and more abundant in the hyperaccumulator. Histochemical tests identiWed these deposits as a mixture of lipids, alkaloids and terpenoids. Specialized cells present in the inner cortex of the hyperaccumulating genotype demonstrated signiWcant relative Ni depletion in comparison with the adjacent inner cortex and phloem. Casparian bands were identiWed in exodermal cell walls of both genotypes but the bands Xuoresced more intensely in the non-accumulator suggesting diVerences in chemical composition and probably also a more eYcient apoplastic barrier. This feature was correlated with the observed Ni distribution pattern. The highest Ni enrichment in the hyperaccumulating genotype occurred in the outer cortex; 20 times more than in the adjacent epidermis/exodermis in older portions of roots and 3 times more than in the epidermis/exodermis in younger root hair regions. In contrast, in the Wojciech Przybyiowicz on leave from the

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Section: Histochemical Testsmentioning

confidence: 99%

Comparison of cytology and distribution of nickel in roots of Ni-hyperaccumulating and non-hyperaccumulating genotypes of Senecio coronatus

2007

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“…Identifying the main groups of metabolites in oil cells: Fresh material was frozen, sectioned and stained with the following reagents: Schiff's reagent (Lewinsohn et al, 1998) and 2, 4-dinitrophenylhydrazine (Liu and Deng, 1979;Ni et al, 2007) for aldehydes; Sudan III (Ruzin, 1999;Rodrigues et al, 2008) and Neutral Red under UV (Ascensao et al, 1999) for total lipids; Nadi reagent for terpenes (Moura et al, 2005); and concentrated H 2 SO 4 for sesquiterpenes (Cappelletti et al, 1986;Nikolakaki and Christodoulakis, 2007). In addition, starch in parenchyma cells and stone cells was identified using I 2 -KI reagent (Ruzin, 1999).…”

Section: Histochemical Investigationsmentioning

confidence: 99%

Histochemistry and Cell Wall Specialization of Oil Cells related to the Essential Oil Accumulation in the Bark ofCinnamomum cassiaPresl. (Lauraceae)

Geng

Cui

Shu

et al. 2012

Plant Production Science

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“…Moreover, the plant fragments were treated with Ruthenium Red for 5 min to determine polysaccharides other than cellulose [Johansen 1940], 1% potassium dichromate for 10 min to detect tannins, and 2% ferric trichloride for 10 min to identify phenolic compounds [Johansen 1940, Soloway and Wilen 1952, Gabe 1968. Furthermore, a reaction with concentrated sulphuric acid was performed to detect sesquiterpenes [Cappelletti et al 1986]. After every reaction, the plant material was washed in distilled water and mounted in a 50% aqueous solution of glycerol.…”

Section: Methodsmentioning

confidence: 99%

HISTOCHEMICAL INVESTIGATION OF TRICHOMES AND CHEMICAL COMPOSITION OF ESSENTIAL OIL FROM Euphrasia stricta D. Wolff ex J.F. Lehm. (OROBANCHACEAE)

Haratym¹,

Weryszko-Chmielewska²

2017

Acta Sci. Pol. Hortorum Cultus

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Micromorphological and anatomical investigations of trichomes on vegetative and generative organs of the drug eyebright (Euphrasia stricta D. Wolff ex J.F. Lehm.) were carried out. Additionally, identification of the main components of the secreted products was conducted. The following types of glandular structures were found: capitate trichomes (i) with a unicellular head, a neck cell, and a 2-celled stalk, (ii) with a unicellular head, a neck cell, and 3-celled stalk, (iii) with a bicellular head and sporadically with a 3-or 4-celled head; moreover, on the surface of corolla unicellular and 2-3-celled clavate trichomes, ribbon-like trichomes, and papillae were present. Using fluorescence microscopy and histochemical tests, various substances such as lipids, phenolic compounds, terpenes containing steroids, terpenoids, sesquiterpenes, tannins, and flavonoids were determined but only in the capitate trichomes. The analysis of the essential oil showed that its content in the dry herb of E. stricta was on average 0.257%. Gas chromatography revealed the presence of 28 compounds.

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Localization of the cytotoxic hydroperoxyeudesmanolides in Artemisia umbelliformis

Cited by 50 publications

References 25 publications

Comparison of cytology and distribution of nickel in roots of Ni-hyperaccumulating and non-hyperaccumulating genotypes of Senecio coronatus

Comparison of cytology and distribution of nickel in roots of Ni-hyperaccumulating and non-hyperaccumulating genotypes of Senecio coronatus

Histochemistry and Cell Wall Specialization of Oil Cells related to the Essential Oil Accumulation in the Bark ofCinnamomum cassiaPresl. (Lauraceae)

HISTOCHEMICAL INVESTIGATION OF TRICHOMES AND CHEMICAL COMPOSITION OF ESSENTIAL OIL FROM Euphrasia stricta D. Wolff ex J.F. Lehm. (OROBANCHACEAE)

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