Localization of the estradiol receptor mRNA in the forebrain of the rainbow trout

Salbert, Gilles; Bonnec, Georgette; Goff, Pascale Le; Boujard, Daniel; Valotaire, Yves; Jégo, Patrick

doi:10.1016/0303-7207(91)90271-s

Cited by 55 publications

(44 citation statements)

References 26 publications

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“…This is clearly different from what has been shown in trout liver by Pakdel et al (1991). Different regulation of rtER mRNA levels according to the cell type in the brain and in the pituitary of rainbow trout has been suggested by Salbert et al (1991). The above in situ hybridization results confirm the absence or undetectable amount of functional ER in the PRL cells of rainbow trout.…”

Section: Discussioncontrasting

confidence: 63%

“…The in situ hybridization protocol has been described by Salbert et al (1991). Briefly, anesthetized trout were intracardially perfused with 4% paraformaldehyde in phosphate-buffered saline (PBS).…”

Section: In Situ Hybridization Analysismentioning

confidence: 99%

“…After inclusion in paraffin wax, serial 6 pm sections were cut and submitted to the in situ hybridization proto& Each slide was covered with 2.66 ng of [35S]UTP-labeled probe (1.5 x 109 cpm,/pg) in 20 ~1 of hybridization buffer. This concentration has been demonstrated to be saturating (Salbert et al, 1991). Hybridization was carried out overnight and sections were then washed, dehydrated and autoradiographed for 10 days.…”

Section: In Situ Hybridization Analysismentioning

confidence: 99%

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Absence of direct regulation of prolactin cells by estradiol-17β in rainbow trout (Oncorhynchus mykiss)

Goff

Salbert

Prunet

et al. 1992

Molecular and Cellular Endocrinology

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SummaryThe effects of estradiol-17/3 (E,) implants on plasma prolactin (PRL) concentrations, pituitary PRL content and pituitary PRL mRNA levels were examined in rainbow trout (Oncorhynchus mykiss). Intact immature fish treated with 1 mg estradiol-17/? did not show significant changes in both PRL mRNA levels and pituitary PRL content after 3 days of treatment. In a similar experiment, no changes were observed in plasma PRL levels followed during 7 days. Similarly, lack of estradiol-17/3 effect on plasma PRL levels and on final PRL pituitary content was observed in ovariectomized female rainbow trout treated during 48 days with 25 mg estradiol-17P and in mature male fish over a 3-week treatment period. Localization of estradiol receptor (ER) mRNAs in the pituitary was carried out by Northern blot analysis using a full-length rainbow trout estrogen receptor (rtER) cDNA as a probe. The rostra1 pars distalis of the pituitary which contained mostly PRL cells showed the lower amount of rtER mRNA when compared to other parts of the pituitary. Moreover, two mRNAs of different size (3.5 and 1.4 kb) were detected in different parts of the pituitary. Further hybridization experiments using probes containing part of the rtER cDNA (E domain or C and D domains) indicated that the small-sized mRNA (1.4 kb) probably encodes a truncated ER protein lacking hormone binding domain or an ER-related protein. Thus, only the 3.56 kb mRNA appeared to be involved in the regulation of pituitary function by estradiol. In situ hybridization analysis allowed a more precise localization of this rtER mRNA in the pituitary. No labeling was discernible over PRL cells whereas other parts of the pituitary were labeled. Grain counting corroborated this localization. These results indicate that (1) in vivo estradiol-17P treatment did not modify PRL cell activity in rainbow trout, (2) PRL secretion, in this fish species, is not directly regulated by estradiol.

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Section: Discussioncontrasting

confidence: 63%

Section: In Situ Hybridization Analysismentioning

confidence: 99%

Section: In Situ Hybridization Analysismentioning

confidence: 99%

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Absence of direct regulation of prolactin cells by estradiol-17β in rainbow trout (Oncorhynchus mykiss)

Goff

Salbert

Prunet

et al. 1992

Molecular and Cellular Endocrinology

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“…The presence of E 2 receptors and E 2 receptor mRNA have been demonstrated in the liver of several teleost species (Le Menn et al, 1980;Slope et al, 1984;Lazier et al, 1985;Pottinger, 1986;McPherson et al, 1988;Pakdel et al, 1989;Pottinger and Pickering, 1990;Smith and Thomas, 1991;Hernández et al, 1992;Campbell et al, 1994;MacKay et al, 1996) and in rainbow trout brain and pituitary (Salbert et al, 1991;Pakdel et al, 1994). Further, a high-affinity E 2 binding component has been identified in teleost plasma (Fostier and Breton, 1975;Le Menn et al, 1980;Lazier et al, 1985;Pasmanik and Callard, 1986;Pottinger, 1986;Pottinger and Pickering, 1990;Laidley and Thomas, 1994).…”

Section: Introductionmentioning

confidence: 96%

The Presence of High-Affinity, Low-Capacity Estradiol-17β Binding in Rainbow Trout Scale Indicates a Possible Endocrine Route for the Regulation of Scale Resorption

Persson

Shrimpton

McCormick³

et al. 2000

General and Comparative Endocrinology

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High-affinity, low-capacity estradiol-17␤ (Etestosterone, but not by diethylstilbestrol. Hence, the ligand binding specificity is different from that of the previously characterized liver E 2 receptor, where E 2 is displaced by diethylstilbestrol, but not by testosterone. The putative scale E 2 receptor thus appears to bind both E 2 and testosterone, and it is proposed that the increased scale resorption observed during sexual maturation in both sexes of several salmonid species may be mediated by this receptor. No high-affinity, low-capacity E 2 binding could be detected in rainbow trout gill or skin.

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“…Thèse studies demonstrated that steroid binding occurred mainly in the ventral telencephalon, the preoptic région, the thalamus and the mediobasal hypothalamus. The availability of new tools to carry out in situ hybridization (ISH ; SALBERT et al, 1991) or immunohistochemistry (IHC ;ANGLADE ef al., 1994) in rainbow trout confirmed and precised the results obtained by autoradiography.…”

Section: Central Areas Target Of Estradiolmentioning

confidence: 99%

The GABAergic system: a possible component of estrogenic feedback on gonadotropin secretion in rainbow trout (Oncorhynchus mykiss)

Anglade

Douard

Jossic-Corcos

et al. 1998

Bull. Fr. Pêche Piscic.

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In teleost fish, GTH sécrétion is controiled by a large number of neuroendocrine factors at the central level and steroid feedback represents an efficient process to synchronize the activity of ail the Systems involved along the brain-pituitary-gonad axis. Estrogen effects are mediated by spécifie nuclear receptors that act as transcription factors to regulate the expression of target gènes. In order to understand the neuroendocrine mechanisms involved in the estrogen feedback on GTH sécrétion, we need, as a first step, to know the nature of target cells at the pituitary and central levels. In rainbow trout, some estrogen receptors expressing cells are identified but the nature of a large number of them remains unknown. In this paper, we explain our strategy to identify the central target of estrogen using both in situ hybridization and immunohistochemistry. We focused our attention on GABA neurons of which the distribution, in some central areas, exactly overlaps with that of estrogen receptors expressing cells.

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Localization of the estradiol receptor mRNA in the forebrain of the rainbow trout

Cited by 55 publications

References 26 publications

Absence of direct regulation of prolactin cells by estradiol-17β in rainbow trout (Oncorhynchus mykiss)

Absence of direct regulation of prolactin cells by estradiol-17β in rainbow trout (Oncorhynchus mykiss)

The Presence of High-Affinity, Low-Capacity Estradiol-17β Binding in Rainbow Trout Scale Indicates a Possible Endocrine Route for the Regulation of Scale Resorption

The GABAergic system: a possible component of estrogenic feedback on gonadotropin secretion in rainbow trout (Oncorhynchus mykiss)

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