Localization of typical and atypical Frankia isolates from Casuarina sp. in nodules formed on Casuarina equisetifolia

Vemulapally, Spandana; Guerra, Trina; Hahn, Dittmar

doi:10.1007/s11104-018-03908-8

Cited by 10 publications

(6 citation statements)

References 53 publications

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“…It is likely that the members of the continental lineage are present on the outside of nodules. This has previously been shown for the cluster-3 species Frankia irregularis [31], members of which had been isolated from nodules of Casuarina species from different continents, but which cannot nodulate the Casuarina genus [32][33][34].…”

Section: Inocula From the Philippines And Japan Can Contain Frankia S...supporting

confidence: 58%

A tale of two lineages: how the strains of the earliest divergent symbiotic Frankia clade spread over the world

Berckx¹,

Nguyen²,

Bandong

et al. 2022

BMC Genomics

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It is currently assumed that around 100 million years ago, the common ancestor to the Fabales, Fagales, Rosales and Cucurbitales in Gondwana, developed a root nodule symbiosis with a nitrogen-fixing bacterium. The symbiotic trait evolved first in Frankia cluster-2; thus, strains belonging to this cluster are the best extant representatives of this original symbiont. Most cluster-2 strains could not be cultured to date, except for Frankia coriariae, and therefore many aspects of the symbiosis are still elusive. Based on phylogenetics of cluster-2 metagenome-assembled genomes (MAGs), it has been shown that the genomes of strains originating in Eurasia are highly conserved. These MAGs are more closely related to Frankia cluster-2 in North America than to the single genome available thus far from the southern hemisphere, i.e., from Papua New Guinea.To unravel more biodiversity within Frankia cluster-2 and predict routes of dispersal from Gondwana, we sequenced and analysed the MAGs of Frankia cluster-2 from Coriaria japonica and Coriaria intermedia growing in Japan, Taiwan and the Philippines. Phylogenetic analyses indicate there is a clear split within Frankia cluster-2, separating a continental from an island lineage. Presumably, these lineages already diverged in Gondwana.Based on fossil data on the host plants, we propose that these two lineages dispersed via at least two routes. While the continental lineage reached Eurasia together with their host plants via the Indian subcontinent, the island lineage spread towards Japan with an unknown host plant.

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Section: Inocula From the Philippines And Japan Can Contain Frankia S...supporting

confidence: 58%

A tale of two lineages: how the strains of the earliest divergent symbiotic Frankia clade spread over the world

Berckx¹,

Nguyen²,

Bandong

et al. 2022

BMC Genomics

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“…The failure to detect nodules has therefore resulted in the assumption that many cluster 4 frankiae are not only ineffective with respect to nitrogen-fixation, but also non-infective with respect to root nodule formation. Cluster 4 frankiae could therefore be characterized as largely or even entirely saprotrophic, with isolates obtained from root nodules as surfaces contaminants 54 .…”

Section: Discussionmentioning

confidence: 99%

Draft genomes of non-nitrogen-fixing Frankia strains

Carlos‐Shanley¹,

Guerra²,

Hahn³

2021

J. Genomics

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In this study, we describe the genomes of two novel candidate species of non-nitrogen fixing Frankia that were isolated from the root nodules of Coriaria nepalensis and Alnus glutinosa , genospecies CN and Ag, respectively . Comparative genomic analyses revealed that both genospecies lack genes essential for nitrogen-fixation and possess genes involved in the degradation of plant cell walls. Additionally, we found distinct biosynthetic gene clusters in each genospecies. The availability of these genomes will contribute to the study of the taxonomy and evolution of actinorhizal symbioses.

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“…Recently other studies have confirmed this nodule surface emplacement of Casuarina incompatible Frankia strains. 35 , 36 …”

Section: Discussionmentioning

confidence: 99%

“…Recently other studies have confirmed this nodule surface emplacement of Casuarina incompatible Frankia strains. 35,36 Alongside with the asymbiotic Frankia strains, the protocol used in the present study permitted the isolation of several other actinobacteria which were affiliated to Micrococcus, Micromonospora, Nocardia, Plantactinospora, and Streptomyces genera. Numerous are the studies which reported the isolation of non-Frankia actinobacteria from actinorhizal root nodules 18,20,34,[37][38][39][40][41][42][43][44][45] as it was shown before for legume nodules which contain both rhizobial and non-rhizobial bacteria.…”

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confidence: 99%

Root Nodule Microsymbionts of Native Coriaria myrtifolia in Algeria

et al. 2022

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Coriaria myrtifolia occurs as natural flora of warm temperate climates of northern Algeria which commonly found in hedges, forest and ravine edges. This actinorhizal species was known to establish a mutualistic symbiosis with members of phylogenetic cluster 2 (including strains associated to Coriaria spp., Ceanothus, Datiscaceae, and Dryadoideae) within the genus Frankia. Attempts to isolate C. myrtifolia microsymbionts from native plants growing in 4 locations in Algeria permitted to only recover asymbiotic Frankia strains (unable to reestablish nodulation and to fix nitrogen) from phylogenetic cluster 4 and several non- Frankia actinobacteria including members of Micrococcus, Micromonospora, Nocardia, Plantactinospora, and Streptomyces genera. The biodiversity of Frankia microsymbionts of C. myrtifolia root nodules was assessed using PCR-amplification followed by partial nucleotide sequencing of glnA1 (glutamine synthetase type 1) gene. On the 12 different glnA1 gene sequences obtained in this study, 9 were detected for the first time, and were mainly closelyrelated to Mediterranean genotypes previously described in the Grand Maghreb countries (Morocco and Tunisia) and in Europe (France) but without clear separations from other cluster 2 genotypes.

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Localization of typical and atypical Frankia isolates from Casuarina sp. in nodules formed on Casuarina equisetifolia

Cited by 10 publications

References 53 publications

A tale of two lineages: how the strains of the earliest divergent symbiotic Frankia clade spread over the world

A tale of two lineages: how the strains of the earliest divergent symbiotic Frankia clade spread over the world

Draft genomes of non-nitrogen-fixing Frankia strains

Root Nodule Microsymbionts of Native Coriaria myrtifolia in Algeria

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