Localizatlon of expansin-like protein in apoplast of pea (Pisum sativum L.) root nodules during interaction with Rhizobium leguminosarum bv. viciae

Abstract

During nodule development on pea roots, apoplast undergoes changes in activity of plant cell wall proteins such as expansins (EXPs). Because the accumulation of EXP protein has been correlated with the growth of various plant organs, we investigated using Western Blot and immunolocalization studies with antibody against PsEXP1, whether this protein was accumulated in the expanding cells of nodule. Immunoblot results indicated the presence of a 30-kDa band specific for pea root nodules. The EXP proteins content rose during growth of pea root nodules. Expansin(s) protein was localized in nodule apoplast as well as in the infection thread walls. The enhanced amount of expansin-like proteins in meristematic part of nodule, root and shoot was shown. The localization of this protein in the meristematic cell walls can be related to the loosening of plant cell wall before cell enlargement. Both, plant cell enlargement and infection thread growth require activity of expansin(s). Possible involvement of EXPs in the process of pea root nodule development is also discussed.

Immunolocalization of α-Expansin Protein (NtEXPA5) in Tobacco Roots in the Presence of the Arbuscular Mycorrhizal Fungus Glomus Mosseae Nicol. & Gerd

“…Expansin proteins have been found in the nodule apoplast as well as in infection thread walls (Sujkowska et al, 2007). Nitrogen-fixing symbiosis (NFS) and AM share a common signal transduction pathway, as microorganisms transfer mineral nutrients to the host plant in both associations, and in both associations the microorganisms are isolated from the host cytoplasm by the apoplastic compartment.…”

INCREASING NODULE SIZE1 Expression Is Required for Normal Rhizobial Symbiosis and Nodule Development

“…For example, the expression of a sweet clover (Melilotus alba) α-expansin gene, MaEXP1, is enhanced within hours of rhizobial infection, and MaEXP1 also is expressed in the nodule cortex, meristem, invasion zone, and interzone II-III (Giordano and Hirsch, 2004). Western-blot analysis revealed that an expansin-like protein, PsEXP1, is localized in IT walls of pea nodules (Sujkowska et al, 2007), suggesting that PsEXP1 might function in IT growth. More recently, a β-expansin gene, GmEXPB2, was found to be critical for soybean nodulation (Li et al, 2015).…”

GmEXPB2, a Cell Wall β-Expansin Gene, Affects Soybean Nodulation through Modifying Root Architecture and Promoting Nodule Formation and Development

“…In roots of white sweet clover (Melilotus alba), the mRNA accumulation of a sweetclover a-expansin gene (MaEXP1) was enhanced within hours of rhizobial inoculation, and the transcript of MaEXP1 was also present in nodule cortex and meristem cells, the invasion zone, and interzone II-III (Giordano and Hirsch, 2004). In pea, western-blot analysis revealed that an expansinlike protein, PsEXP1, was localized in nodule apoplasts and IT walls, suggesting that PsEXP1 might be required for nodule cell and IT growth (Sujkowska et al, 2007). Although transcriptional analysis of genes involved in soybean nodulation have been carried out (Carvalho et al, 2013), details of gene functions during nodulation remain poorly understood.…”