The 16MnCr5 alloy steel samples are evaluated in a normalized condition and put through low cycle fatigue (LCF) under constant amplitude strains of εa = 0.2%, 0.25%, 0.4%, and 0.6%. Mechanical properties are reported based on the LCF results. Fatigue damage is checked in specimens that have reached 80% of their nominal fatigue life by two methods that are different from traditional electronic microscopy: measuring the roughness of the surface and looking at it through full‐field optical microscopy. In addition, the electric resistivity is determined for the test specimens. It presents an increment from the blank value of 6.5 × 10−8 Ωm (free of fatigue damage) to 1.25 × 10−7 Ωm for the fatigue test specimen (εa = 0.6%). Finally, X‐ray diffraction patterns are determined for analyzing the residual equivalent strain at the microscale for the fatigued samples. The residual equivalent strain exhibits a trend toward increasing. For instance, it increases from 18.5 (blank value) to 34.0 με for the fatigue test specimen with εa = 0.6%.