Location and Properties of an Esterase Activity in Saccharomyces cerevisiae

A novel esterase fromSaccharomyces carlsbergensis, a possible function for the yeastTIP1 gene

“…The S. carlsbergensis esterase was not active at pH 7·5 (see Figure 1), indicating that the S. cerevisiae cell wall esterase is different from the esterase purified from the culture supernatant of S. carlsbergensis. The pH optimum of the previously identified cell wall esterase was 6·5 or 8 (Wheeler and Rose, 1973;Parkkinen et al, 1978), suggesting that this is the esterase activity which is assayed at pH 7·5 in the cell wall preparation of S. cerevisiae.…”

Physiological properties and plasma membrane composition of Saccharomyces cerevisiae grown in sequential batch culture and in the presence of surfactants

“…Addition of lecithin or Tween 80 (two sources of unsaturated fatty acids) in culture medium during S. carlsbergensis in sequential-batch culture without aeration increased the viability of this yeast from 70 to 100% (Thompson and Ralph 1967). Esterified fatty acids and in particular oleie acid act after their enzymatic liberation (Wheeler and Rose 1973;Panchal and Stewart 1980) as growth factor (Lands and Graff 1980). The inhibition of foaming during the three fermentation cycles indicated the excellent antifoam action of the various PAG tested, taking into account the low concentration used.…”

The Effects of Catabolite Derepression on the Accumulation of Steryl Esters and the Activity of -Hydroxymethylglutaryl-CoA Reductase in Saccharomyces cerevisiae

“…An anaerobic yeast sterol esterase has been demonstrated by Wheeler & Rose (1973) which may be responsible for the conversion.…”