Location of the 18S and 28S rRNA cistrons in the genome of the domestic rabbit (&lt;i&gt;Oryctolagus cuniculus&lt;/i&gt; L.)

Pa, Martin-DeLeon

doi:10.1159/000131509

Cited by 18 publications

(10 citation statements)

References 5 publications

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“…Our data are in agreement with previous studies in the rabbit in which nucleolar organizer regions were identified at the same locations by sequential Qbanding and silver staining (Martin-DeLeon et al, 1978). Furthermore, the present data confirm the localization that was achieved by hybridization of RNA probes in radioactive in situ hybridization experiments (Martin-DeLeon, 1980). Hybridization with DNA isolated from BAC clones resulted, in general, in clear fluorescent spots.…”

Section: Resultssupporting

confidence: 93%

Fourteen chromosomal localizations and an update of the cytogenetic map of the rabbit

Zijlstra¹,

Haan²,

Korstanje

et al. 2002

Cytogenet Genome Res

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In order to improve the informativeness of the cytogenetic map of the rabbit genome, fourteen markers were regionally mapped to individual chromosomes. The localizations comprise eleven gene loci (PRLR, GHR, HK1, ACE, TF, 18S+28S rDNA, CYP2C4, PMP2, TCRB, ALOX15 and MT1) and three microsatellite loci (Sat13, Sol33 and D1Utr6). Five of the genes contain known microsatellite sequences. To achieve these localizations, homologous and heterologous small insert clones, and clones from a rabbit Bacterial Artificial Chromosome (BAC) library were used as probes for fluorescence in situ hybridization experiments. Results indicate that especially BAC clones are a valuable tool for cytogenetic mapping. Some of the genes were selected for mapping on the basis of human- rabbit comparative painting data, to achieve localizations on gene-poor rabbit chromosomes. Our data are, in general, in agreement with the human-rabbit comparative painting data. By mapping microsatellite sequences that have also been used in linkage studies, links are provided between the genetic and physical maps of the rabbit genome. Linkage groups I, VI and XI could be assigned to chromosomes 1, 5 and 3 respectively. Moreover, in this paper we give an overview of the current status of the rabbit cytogenetic map. This map now comprises 62 physically mapped genes, which are scattered over all autosomes, except chromosome 2, and the X chromosome.

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Section: Resultssupporting

confidence: 93%

Fourteen chromosomal localizations and an update of the cytogenetic map of the rabbit

Zijlstra¹,

Haan²,

Korstanje

et al. 2002

Cytogenet Genome Res

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“…In a great number of mammalian species, the location of the genes coding for 18s and 28s ribosomal RNA (rRNA genes or rDNA) has been determined by in situ hybridization (Evans et al 1974;Henderson et al 1974;Hsu et al 1975;Martin-DeLeon 1980). In the domestic pig, application of this method has revealed that the rRNA genes are located either in the secondary constriction of chromosome no.…”

Section: Introductionmentioning

confidence: 99%

Numerical variation of nucleolar organizer regions after silver staining in domestic and wild Suidae (Mammalia)

et al. 1992

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Selective silver staining was used to investigate the cellular distribution of numbers of nucleolar organizer regions (NORs) in domestic pigs (Sus scrofa) of eight different breeds, the European wild boar (S. scrofa scrofa), Indonesian wild boar (S. scrofa vittatus), Javan warty pig ( S . verrucosus), Sulawesi warty pig (S. celebensis), and pigmy hog (S. salvanius). In the domestic pig as well as in the wild (sub)species of Sus, actively transcribing ribosomal RNA genes were found to be present in the secondary constrictions of chromosome pairs 10 and 8. Chromosomes 10 were consistently Ag-positive. Chromosomes 8 less frequently showed Ag-NORs, resulting in different mean numbers of Ag-NORs per individual animal. Mean Ag-NOR numbers per breed or (sub)species were generally higher in the wild representatives of Sus than in the domestic breeds. The highest mean numbers of Ag-NORs were observed in the Meishan breed and in S. celebensis and S. salvanius.The Meishan breed appears to be conservative in Ag-NOR staining pattern, being more comparable to the Asian wild Suidae than to the European breeds.

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“…The only possible comparison of Ag-NOR position is with Oryctolagus cuniculus (Martin-DeLeon 1980 ;Martin-DeLeon et al 1978 ;Monteagudo and Arruga 1991); this species shows the occurence of Ag-NORs in three pairs of chromosomes which might correspond to those of L. starcki, but also in a pair of small acrocentrics which do not find any homologue in our data.…”

Section: Resultsmentioning

confidence: 51%

Karyotypes of two endemic species of hare from Ethiopia, Lepus habessinicus and L. starcki (Lagomorpha, Leporidae). A comparison with L. europaeus

Puccetti¹,

Corti²,

Scanzani³

et al. 1996

Mammalia

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Karyotypes of two endemic species of hare from Ethiopia, Lepus habessinicus and L. starcki were studied and compared to that of L. europaeus. L. starcki has a fragmented range in the high Ethiopian plateaus and is considered a relict form of L. europaeus. In contrast, L. habessinicus occurs at lower altitudes, and is an arid-country species. The species share a 2n = 48 and a FNa = 88 common to all representatives of the genus. No sign of karyotypic differentiation is evident from G-banding and Ag-NOR location. These data support the hypothesis of pronounced karyotypic conservativism in the genus Lepus.Resume. -On a etudie le caryotype de deux especes de lievres endemiques d'Ethiopie, Lepus habessinicus et Lepus starcki et on les a compares ä celui de Lepus europaeus. L. starcki est distribue irregulierement sur le plateau ethiopien et est considere comme une forme relicte de L. europaeus, tandis que L. habessinicus vit ä des altitudes plus basses et est considere comme une espece xerophile. Les especes ethiopiennes partagent le meme caryotype ä 48 chromosomes (NFa = 88), commun a l'ensemble du genre Lepus. L'analyse des bandes G et Ag-NOR n'a mis en evidence aucune difference caryotypique entre les 3 especes. Ces donnoes confirment 1'hypothese que, chez les lievres, le caryotype est fortement conserve.

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Location of the 18S and 28S rRNA cistrons in the genome of the domestic rabbit (<i>Oryctolagus cuniculus</i> L.)

Cited by 18 publications

References 5 publications

Fourteen chromosomal localizations and an update of the cytogenetic map of the rabbit

Fourteen chromosomal localizations and an update of the cytogenetic map of the rabbit

Numerical variation of nucleolar organizer regions after silver staining in domestic and wild Suidae (Mammalia)

Karyotypes of two endemic species of hare from Ethiopia, Lepus habessinicus and L. starcki (Lagomorpha, Leporidae). A comparison with L. europaeus

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