Locomotion in larval zebrafish: Influence of time of day, lighting and ethanol

MacPhail, Robert C.; Brooks, Janie S.; Hunter, Deborah L.; Padnos, Beth; Irons, T.D.; Padilla, Stephanie

doi:10.1016/j.neuro.2008.09.011

Cited by 398 publications

(324 citation statements)

References 31 publications

Supporting

Mentioning

289

Contrasting

Unclassified

Order By: Relevance

“…This typical behavior in the 31.0 mg/L group of low activity in normal light but hyperactivity in dark has also been observed in F1 larvae after long-term adult exposure to BDE-209 [22]. In addition, our results showed that PBDE exposure was associated with delayed transition in activity following photoperiod transition, which was also reported for 144 hpf zebrafish larvae treated with 1% ethanol [33]. Although the mechanisms of neurobehavioral alterations are still not well understood, it has been proposed that disruption of the cholinergic system or interactions with neurotransmitter systems or the development of the neuron may explain the neurotoxicity of PBDEs [1,36].…”

Section: Discussionsupporting

confidence: 86%

“…However, during the photoperiod stimulation test, the larvae exposed to 31.0 mg/L DE-71 elicited a higher level of basal activity during the dark period, whereas the basal activity in the 68.7 and 227.6 mg/L groups was decreased. This hyperactivity with low concentration but hypoactivity with high concentration in the dark period has also been observed in zebrafish larvae treated with ethanol [33] and neuroactive drugs [34]. This phenomenon, hormesis, is widespread throughout different organisms when they are exposed to toxicants and is thought to be an adaptive response by which the body protects itself from toxicant stress [35].…”

Section: Discussionmentioning

confidence: 75%

See 1 more Smart Citation

Acute exposure to DE‐71: Effects on locomotor behavior and developmental neurotoxicity in zebrafish larvae

Chen

Huang

et al. 2012

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

Abstract-The aim of the present study was to investigate the acute developmental neurotoxicity of polybrominated diphenyl ethers (PBDEs) in zebrafish larvae. From 2 to 120 h postfertilization zebrafish embryos were exposed to 31.0, 68.7, and 227.6 mg/L). The authors studied the locomotor behavior of larvae, involvement of the cholinergic system, and selected gene and protein expressions in the central nervous system. The results showed that low DE-71 concentration caused hyperactivity, whereas higher concentrations decreased activity during the dark period. During the light period, larval activity was significantly reduced in a concentration-dependent manner. In the cholinergic system, acetylcholinesterase activity significantly increased (10.7 and 12.4%) in the 68.7 and 227.6 mg/L exposure groups, respectively, and acetylcholine concentration accordingly decreased (60.5%) in the 227.6 mg/L exposure group. The mRNA expressions of genes encoding myelin basic protein, neuron microtubule protein (a1-tubulin), and sonic hedgehog a were significantly downregulated. Western blotting assay demonstrated that the protein concentration of a1-tubulin was also decreased.Overall, the present study demonstrated that acute exposure to PBDEs can disrupt the neurobehavior of zebrafish larvae and affect cholinergic neurotransmission and neuron development. Environ. Toxicol. Chem. 2012;31:233831: -234431: . # 2012

show abstract

Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 75%

Acute exposure to DE‐71: Effects on locomotor behavior and developmental neurotoxicity in zebrafish larvae

Chen

Huang

et al. 2012

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

show abstract

“…A series of recent studies (MacPhail et al, 2009;Staddon et al 2010) have shown successive induction in the activity patterns of tiny (larval -6 days post-fertilization) zebrafish (Danio rerio). These little fish, still living on their yolk sacs, show movement at a low rate in the con-tinuous dark and intermediate in continuous light.…”

Section: Figure 213mentioning

confidence: 99%

Adaptive Behavior and Learning

Staddon

2016

View full text Add to dashboard Cite

Every day at about 4:30, Jazz, a Hungarian Vizsla dog, leaps up on the sofa and looks out for his owner who always comes home at 5:00. He doesn't need an internal clock because he has an acute sense of smell that allows him to measure how long his master has been absent. Explaining complex behavior in simple ways, this book is a fascinating exploration of the evolution, development and processes of learning in animals. Now in its second edition, there is increased emphasis on development, evolution and dynamics; new accounts of taxic orientation, reflex induction, habituation and operant learning in organisms; more discussion of spatial learning and the processes underlying it; expanded chapters on choice and completely new chapters on molar laws, classical conditioning theories and comparative cognition. J. E. R. Staddon provides a definitive summary of contemporary theoretical understanding suitable for graduates and advanced undergraduates.

show abstract

“…The locomotor activity of zebrafish larvae in the dark is known to be higher than in the light (MacPhail et al, 2009). Therefore, the measurement of amount of activity was conducted in the dark.…”

Section: Measurement Of Locomotor Activitymentioning

confidence: 99%

Establishment of a novel experimental protocol for drug-induced seizure liability screening based on a locomotor activity assay in zebrafish

et al. 2014

View full text Add to dashboard Cite

-As drug-induced seizures have severe impact on drug development, evaluating seizure induction potential of candidate drugs at the early stages of drug discovery is important. A novel assay system using zebrafish has attracted interest as a high throughput toxicological in vivo assay system, and we tried to establish an experimental method for drug-induced seizure liability on the basis of locomotor activity in zebrafish. We monitored locomotor activity at high-speed movement (> 20 mm/sec) for 60 min immediately after exposure, and assessed seizure liability potential in some drugs using locomotor activity. However this experimental procedure was not sufficient for predicting seizures because the potential of several drugs with demonstrated seizure potential in mammals was not detected. We, therefore, added other parameters for locomotor activity such as extending exposure time or conducting flashlight stimulation (10 Hz) which is a known seizure induction stimulus, and these additional parameters improved seizure potential detection in some drugs. The validation study using the improved methodology was used to assess 52 commercially available drugs, and the prediction rate was approximately 70%. The experimental protocol established in this present study is considered useful for seizure potential screening during early stages of drug discovery.Key words: Zebrafish, Locomotor activity, Seizure, Extending exposure time, Flashlight stimulation Correspondence: Naoteru Koseki (E-mail: naoteru-koseki@ds-pharma.co.jp) Original ArticleThe Journal of Toxicological Sciences (J. Toxicol. Sci.) Vol.39, No.4, 579-600, 2014 Vol. 39 No. 4 579 through the blood-brain barrier (BBB). Since such assays are conducted with a focus on specific neural networks or cells associated with seizures, performing an evaluation using above alternative methods is direct and limited. On the basis of the aforementioned findings, a test system that can take pharmacokinetic effects into account and can be used to perform an evaluation under conditions closer to whole body is considered desirable. As such, an in vivo evaluation system using Caenorhabditis elegans (C. elegans), which is one of the nematodes with gamma-aminobutyratergic (GABAergic) neurons related to seizures, has been reported (Locke et al., 2006;Pandey et al., 2010), and an evaluation using behavioral assessment of such organism is expected. However, nematodes are very different from mammals in terms of the anatomical structure of central nervous system (CNS) or pharmacokinetics. Therefore, performing an assessment which approximates the evaluation of a conventional study using rodents is considered to be difficult.Zebrafish is a vertebrate and its genomic homology to humans is approximately 70% (Howe et al., 2013). In addition, zebrafish can be utilized in early stage drug screening in recent decades. For these reasons, an evaluation using zebrafish has recently been given attention as a novel in vivo evaluation system for safety assessment. Since zebrafish have tele...

show abstract

Locomotion in larval zebrafish: Influence of time of day, lighting and ethanol

Cited by 398 publications

References 31 publications

Acute exposure to DE‐71: Effects on locomotor behavior and developmental neurotoxicity in zebrafish larvae

Acute exposure to DE‐71: Effects on locomotor behavior and developmental neurotoxicity in zebrafish larvae

Adaptive Behavior and Learning

Establishment of a novel experimental protocol for drug-induced seizure liability screening based on a locomotor activity assay in zebrafish

Contact Info

Product

Resources

About