Locomotor behavior changes induced by E-17 striatal transplants in normal rats

Hagenmeyer-Houser, Starr H.; Sanberg, Paul R.

doi:10.1016/0091-3057(87)90372-8

Cited by 16 publications

(1 citation statement)

References 13 publications

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“…This means that there may not be adequate survival signals to keep the graft alive as well as the surrounding `normal' tissue. Previous studies of fetal grafts, or marrow stromal cells into normal brain tissue do show some evidence of detrimental effects [7,18,30] and therefore it may be interesting to see whether HUCB cell survival is enhanced following transplantation into lesioned NOD SCID mice. Coyne et al [7] found that marrow stromal cell transplants induced an inflammatory response which led to loss of the transplanted cells by 14 days, and Hagenmeyer-Houser and Sanberg [18] showed a time-dependent decrease in striatal transplant size of embryonic striatal tissue at 10 weeks compared to 3 weeks, though they still had a surviving graft at the later time point.…”

Section: Discussionmentioning

confidence: 99%

Long-term cultured human umbilical cord neural-like cells transplanted into the striatum of NOD SCID mice

Walczak¹,

Chen²,

Eve³

et al. 2007

Brain Research Bulletin

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The use of stem cells and other cells as therapies is still in its infancy. One major setback is the limited survival of the grafts, possibly due to immune rejection. Studies were therefore performed with human umbilical cord blood cells (HUCB) to determine the ability of these cells to survive in vivo and the effect of the immune response on their survival by transplantation into the normal striatum of immunodeficient NOD SCID mice.Long-term culture of HUCB cells resulted in several different populations of cells, including one that possessed fine processes and cell bodies that resembled neurons. Their neuronal phenotype was confirmed by immunohistochemical staining for the early neuronal marker TuJ1 and the potentially neural marker Nestin. Five days after cell transplantation of this neuronal phenotype, immunohistochemical staining for human mitochondria confirmed the presence of living HUCB cells in the mouse striatum, with cells localized at the site of injection, expressing early neural and neuronal markers (Nestin and TuJ1) as well as exhibiting neuronal morphology. However, no evidence of surviving cells was apparent 1 month postgrafting. The absence of signs of T cell-mediated rejection, such as CD4 and CD8 lymphocytes and minimal changes in microglia and astrocytes, suggest that cell loss was not due to a T cell-mediated immune response. In conclusion HUCB cells can survive long-term in vitro and undergo neuron-like differentiation. In mice, these cells do not survive a month. This may relate to the differentiated state of the cells transplanted into the unlesioned striatum, rather than T cell-mediated immunological rejection.

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