Logic‐Gated Proximity Aptasensing for Cell‐Surface Real‐Time Monitoring of Apoptosis

Zheng, Jiao; Wang, Qiwei; Shi, Lin; Peng, Pai; Shi, Lili; Li, Tao

doi:10.1002/anie.202106651

Cited by 54 publications

(52 citation statements)

References 82 publications

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Section: Identification Of Tumor Markers On Cell Membranementioning

confidence: 99%

“…Li and coworkers introduced triplex‐boosted G‐quadruplexs (tb‐G4s) composed of pH‐dependent triplexes and the stem‐loop motifs of bimolecular G‐quadruplexes. [ 115 ] This H + and K + logic responsive tb‐G4s was used to construct a framework nucleic acid (FNA) nanoplatforms with separated ATP aptamers. Once anchored onto cancer cell surfaces by membrane protein, the FNA nanoplatforms perform in situ monitoring of intracellular ATP molecules secreted to the cell membrane surface (Figure 5c).…”

Section: Application Of Dna Logic Circuit In Tumor Diagnosismentioning

confidence: 99%

mentioning

confidence: 99%

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DNA Logic Circuits for Cancer Theranostics

Chen

Zhang

et al. 2022

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solutions for precise cancer theranostics based on tumor microenvironment (TME) and biomarkers. [3][4][5][6][7] Nano-drugs such as liposomes, [8] polymer nanoparticles, [9] and inorganic nanoparticles [10] have been approved by Food and Drug Administration for the clinical treatment of cancer. However, current nanomedicine technologies lack intelligence which is the capability of autonomous computing in biological samples. Owing to the basepairing language and dynamic assembly of DNA strands, DNA-based logic devices/ circuits hold great potential to enhance the intelligence of nanomedicine.DNA nanotechnology began with the DNA four-arm junction structure proposed by Seeman in the 1980s. [11] On the basis of the Watson-Crick base pairing principle, such nanotechnology creates a variety of controllable nanoscale structures based on self-assembly of nucleic acid, and then built up static DNA nano structures such as DNA tiles and DNA origami by bottom-up approach. [12] The development of static DNA nanotechnology creates highly stable and variable DNA nanostructures showing the applications in nanomedicine including molecular imaging, drug delivery, biosensors, and the revolution of data storage. [13][14][15][16][17][18] Because nucleic acid materials have excellent structural controllability, biosafety, and functional diversity, they have emerged as excellent materials for the early diagnosis and timely treatment of cancer. [19] DNA nanotechnology had been further developed by Yurke et al. They constructed the first DNA molecular machine and proposed the concept of toehold-mediated strand displacement (TMSD) for the first time, [20] which opened a new era of dynamic DNA nanotechnology. Dynamic DNA nanotechnology utilizes a nonequilibrium nucleic acid system based on TMSD [21] or enzymatic reactions, to develop DNA nanomechanical devices, [22] and molecular logic networks. [23][24][25][26][27][28] Through the cascade and integration of multiple DNA reaction modules, DNA logic circuit is developed for cancer theranostics. [29,30] The basic DNA logic circuit can be conceptualized as a black box, which accepts the DNA strand as input and implements signal conversion by performing modular circuit elements such as TMSD and enzymatic reaction, [31] finally releases the DNA strand as output. [32] In cancer theranostics, DNA logic circuits can respond to the microenvironment or specific biomarkers of tumor, through signal transduction, logical judgment, signal amplification, Cancer diagnosis and therapeutics (theranostics) based on the tumor microenvironment (TME) and biomarkers has been an emerging approach for precision medicine. DNA nanotechnology dynamically controls the self-assembly of DNA molecules at the nanometer scale to construct intelligent DNA chemical reaction systems. The DNA logic circuit is a particularly emerging approach for computing within the DNA chemical systems. DNA logic circuits can sensitively respond to tumor-specific markers and the TME through logic operations and signal amplification, to genera...

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Section: Identification Of Tumor Markers On Cell Membranementioning

confidence: 99%

Section: Application Of Dna Logic Circuit In Tumor Diagnosismentioning

confidence: 99%

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DNA Logic Circuits for Cancer Theranostics

Chen

Zhang

et al. 2022

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“…[24,25] In particular, anti-ATP aptamer, [26,27] a functional DNA with structure-switching activity, is showing immense promise in engineering probes for ATP sensing and imaging. [10,21,[27][28][29][30] Despite the progress made, most of these efforts are limited to the imaging of intracellular ATP. Most recently, a proximity-dependent aptamer sensor was designed for real-time imaging of extracellular ATP.…”

mentioning

confidence: 99%

“…Most recently, a proximity-dependent aptamer sensor was designed for real-time imaging of extracellular ATP. [30] Li and coworkers reported the specific imaging of extracellular ATP with a self-phosphorylating DNAzyme. [31] We previously presented a conceptual method for the design of proteasetriggered aptamer sensors via using PNA to bridge the gap between functional DNA and peptide, which is further demonstrated for specific ATP imaging in cancer cells.…”

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confidence: 99%

A Multivariate‐Gated DNA Nanodevice for Spatioselective Imaging of Pro‐metastatic Targets in Extracellular Microenvironment

Xiang

Zhao

et al. 2021

Angew Chem Int Ed

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Probing pro‐metastatic biomarkers is of significant importance to evaluate the risk of tumor metastasis, but spatially selective imaging of such targets in extracellular microenvironment is particularly challenging. By introducing the bilinguality of PNA/peptide hybrid that can speak both peptide substrate and nucleobase‐pairing languages to combine with aptamer technology, we designed a smart DNA nanodevice programmed to respond sequentially to dual pro‐metastatic targets, MMP2/9 and ATP, in extracellular tumor microenvironment (TME). The DNA nanodevice is established based on the combination of an ATP‐responsive aptamer sensor and a MMP2/9‐hydrolyzable PNA/peptide copolymer with a cell membrane‐anchoring aptamer module. Taking 4T1 xenograft as a highly aggressive tumor model, the robustness of the DNA nanodevice in spatioselective imaging of MMP2/9 and ATP in TME is demonstrated. We envision that this design will enable the simultaneous visualization of multiple pro‐metastatic biomarkers, which allows to gain insights into their pathological roles in tumor metastasis.

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Logic Gate Activated Lysosome Targeting DNA Nanodevice for Controlled Proteins Degradation

Shang,

Zhu,

Xiao

et al. 2023

Adv Funct Materials

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Targeted protein degradation (TPD) is a powerful technique for the regulation of protein homeostasis. Current TPD mainly focuses on the therapeutical consequences rather than the operation processes of the molecular tools. Herein, a platform for precise manipulation of the protein degradation by activatable lysosome targeting DNA nanodevices is constructed. In the design, a lysosome‐targeting CD63 aptamer is locked by the single‐stranded DNA with a photocleavable group and a disulfide bond. This locked CD63 aptamer is connected with the aptamer targeting the protein of interest via double‐stranded DNA linkages to form the logic‐gate activated lysosome targeting DNA nanodevice (LALTD). With the UV light and endogenous GSH as inputs, an AND logic gate is built to efficiently manipulate the protein delivery processes by LALTD. The protein of interest can be degraded via efficient lysosome hydrolysis. Further studies show that the logic‐gate operation can be used for modulating T cell‐mediated antitumor immunity. The modularly designed activatable lysosome targeting DNA nanodevices exhibits good stability, controllability, programmability, and universality, providing a new prospect for accurate protein degradation and precise therapy.

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Logic‐Gated Proximity Aptasensing for Cell‐Surface Real‐Time Monitoring of Apoptosis

Cited by 54 publications

References 82 publications

DNA Logic Circuits for Cancer Theranostics

DNA Logic Circuits for Cancer Theranostics

A Multivariate‐Gated DNA Nanodevice for Spatioselective Imaging of Pro‐metastatic Targets in Extracellular Microenvironment

Logic Gate Activated Lysosome Targeting DNA Nanodevice for Controlled Proteins Degradation

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