Long Chain Acyl-Coenzyme A Synthetase 4 (BnLACS4) Gene from Brassica napus Enhances the Yeast Lipid Contents

Tan, Xiao‐Li; Zheng, Xizhong; Zhang, Zhiyan; Zheng, Wenhua; Xia, Hengchuan; Lu, Changming; Gu, Shoulai

doi:10.1016/s2095-3119(13)60583-3

Cited by 18 publications

(15 citation statements)

References 37 publications

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“…Then, acyl-ACP thioesterase (FAT) releases fatty acids from ACP. Free fatty acids are activated to CoA esters by long-chain acyl-CoA synthetases (LACS) before their transport into the endoplasmic reticulum. , …”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis of Walnut (Juglans regia L.) Embryos Reveals Key Developmental Stages and Genes Involved in Lipid Biosynthesis and Polyunsaturated Fatty Acid Metabolism

Huang

Zhou

Zhang

et al. 2020

J. Agric. Food Chem.

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Walnut (Juglans regia L.) is a widely cultivated woody oilseed tree species, and its embryo is rich in polyunsaturated fatty acids. Thus far, the pathways and essential genes involved in oil biosynthesis in developing walnut embryos remain largely unclear. Our analyses revealed that a mature walnut embryo accumulated 69% oil, in which 71% were polyunsaturated fatty acids with 64% linoleic acid and 7% linolenic acid. RNA sequencing generated 39 384 unigenes in 24 cDNA libraries prepared from walnut embryos collected at 49, 63, 77, 91, 105, 119, 133, and 147 days after pollination (DAP). The principal components analysis (PCA) of samples and cluster analysis of differentially expressed genes (DEGs) showed that the total samples were divided into three main groups: 49 DAP, 63−119 DAP, and 133−147 DAP. We identified 108 unigenes associated with lipid biosynthesis, including 60 unigenes for fatty acid biosynthesis, 33 for triacylglycerol biosynthesis, 7 for oil bodies, and 8 for transcription factors. The expression levels of the genes encoding WRI1, ACCase, ACP, KASII, SAD, FAD2, FAD3, and PDAT were upregulated at 63− 119 DAP relative to the levels at 49 DAP. Additionally, the lipid biosynthesis in walnut embryos began to increase while oil contents increased from 15 to 69%. We identified eight SAD, three FAD2, one FAD3, one FAD5, one FAD6, and three FAD7/8 genes. In addition, SAD, FAD2, and FAD3 were highly abundantly expressed in the walnut embryo, and their FPKM values achieved were 834, 2205, and 9038, respectively. High expression levels of FAD2 and FAD3 may be the reason why walnuts are rich in polyunsaturated fatty acids. Subcellular localization confirmed that the JrFAD3 protein played a role in the endoplasmic reticulum rather than the plastid, suggesting that linolenic acid was mainly synthesized in the endoplasmic reticulum. Weighted gene coexpression network analysis (WGCNA) showed that ACP, ENO, VAMP727, and IDD14 were coexpressed with WRI1. Our study provides large-scale and comprehensive transcriptome data of walnut embryo development. These data lay the foundation for the metabolic engineering of walnuts to increase oil contents and modify fatty acid compositions.

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Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis of Walnut (Juglans regia L.) Embryos Reveals Key Developmental Stages and Genes Involved in Lipid Biosynthesis and Polyunsaturated Fatty Acid Metabolism

Huang

Zhou

Zhang

et al. 2020

J. Agric. Food Chem.

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“…For example, overexpression of endogenous LACS genes in E. coli has been used to boost the production of fatty esters suitable for biodiesel, as well as wax esters used in the manufacture of soaps and surfactants (Steen et al, 2010). Overexpressing LACS genes from Arabidopsis and Brassica napus improves storage lipid accumulation in yeast (Pulsifer et al, 2012;Tan et al, 2014). However, preferential activation of undesirable acyl chain lengths, low activity of LACS enzymes in heterologous hosts, or the inability of such hosts to efficiently incorporate acyl-CoA thioesters of certain chain lengths into larger lipid molecules, are major bottlenecks in the sustainable biological production of valuable lipids (Xu et al, 2019).…”

Section: Engineering Recombinant Fatty Acyl-acp Thioesterases and Fatmentioning

confidence: 99%

“…Although total oil content as a percentage of seed weight in the lacs4 lacs8 mutant does not differ significantly from wild‐type, seed size is greatly reduced in this mutant, leading to a large decrease in total fatty acid content per seed (Zhao et al, ). Bn LACS4, a LACS4 orthologue from Brassica napus , has also been shown to function in seed oil biosynthesis, and Eg LACS1, a LACS1 orthologue from oil palm ( Elaeis guineensis ) is involved in oil accumulation in the fruit mesocarp (Chong et al, ; Tan et al, ; Zheng et al, ).…”

Section: Major Roles Of Fatty Acyl Synthetases In Plantsmentioning

confidence: 99%

Fatty Acyl Synthetases and Thioesterases in Plant Lipid Metabolism: Diverse Functions and Biotechnological Applications

Kalinger

Pulsifer

Hepworth

et al. 2020

Lipids

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Plants use fatty acids to synthesize acyl lipids for many different cellular, physiological, and defensive roles. These roles include the synthesis of essential membrane, storage, or surface lipids, as well as the production of various fatty acid-derived metabolites used for signaling or defense. Fatty acids are activated for metabolic processing via a thioester linkage to either coenzyme A or acyl carrier protein. Acyl synthetases metabolically activate fatty acids to their thioester forms, and acyl thioesterases deactivate fatty acyl thioesters to free fatty acids by hydrolysis. These two enzyme classes therefore play critical roles in lipid metabolism. This review highlights the surprisingly complex and varying roles of fatty acyl synthetases in plant lipid metabolism, including roles in the intracellular trafficking of fatty acids. This review also surveys the many specialized fatty acyl thioesterases characterized to date in plants, which produce a great diversity of fatty acid products in a tissue-specific manner. While some acyl thioesterases produce fatty acids that clearly play roles in plant-insect or plant-microbial interactions, most plant acyl thioesterases have yet to be fully characterized both in terms of their substrate specificities and their functions. The biotechnological applications of plant acyl thioesterases and synthetases are also discussed, as there is significant interest in these enzymes as catalysts for the sustainable production of fatty acids and their derivatives for industrial uses.

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“…Therefore, there is a need to maximize the productivity of vegetable oil to solve the problem of edible oil supply and relieve pressure on energy supply (Chauhan et al ., ). Rapeseed oil is a crucial source of vegetable oil, and currently, many studies have focused on enhancing the synthetic activity of oil to elevate the oil content (Li et al ., , Tan et al ., ). However, lipid synthesis efficiency is limited, and achieving oil content to 50%–55% in B. napus seems to be a natural limit (Li et al ., ).…”

Section: Introductionmentioning

confidence: 97%

Down‐regulation of BnDA1, whose gene locus is associated with the seeds weight, improves the seeds weight and organ size in Brassica napus

Jieli

Tang

Chen

et al. 2017

Plant Biotechnology Journal

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Summary Brassica napus L. is an important oil crop worldwide and is the main raw material for biofuel. Seed weight and seed size are the main contributors to seed yield. DA1 (DA means big in Chinese) is an ubiquitin receptor and negatively regulates seed size. Down‐regulation of AtDA1 in Arabidopsis leads to larger seeds and organs by increasing cell proliferation in integuments. In this study, BnDA1 was down‐regulated in B. napus by over expressed of AtDA1 R358K, which is a functional deficiency of DA1 with an arginine‐to‐lysine mutation at the 358th amino acid. The results showed that the biomass and size of the seeds, cotyledons, leaves, flowers and siliques of transgenic plants all increased significantly. In particular, the 1000 seed weight increased 21.23% and the seed yield per plant increased 13.22% in field condition. The transgenic plants had no negative traits related to yield. The candidate gene association analysis demonstrated that the BnDA1 locus was contributed to the seeds weight. Therefore, our study showed that regulation of DA1 in B. napus can increase the seed yield and biomass, and DA1 is a promising target for crop improvement.

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Long Chain Acyl-Coenzyme A Synthetase 4 (BnLACS4) Gene from Brassica napus Enhances the Yeast Lipid Contents

Cited by 18 publications

References 37 publications

Transcriptome Analysis of Walnut (Juglans regia L.) Embryos Reveals Key Developmental Stages and Genes Involved in Lipid Biosynthesis and Polyunsaturated Fatty Acid Metabolism

Transcriptome Analysis of Walnut (Juglans regia L.) Embryos Reveals Key Developmental Stages and Genes Involved in Lipid Biosynthesis and Polyunsaturated Fatty Acid Metabolism

Fatty Acyl Synthetases and Thioesterases in Plant Lipid Metabolism: Diverse Functions and Biotechnological Applications

Down‐regulation of BnDA1, whose gene locus is associated with the seeds weight, improves the seeds weight and organ size in Brassica napus

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