Long-chain polyunsaturated fatty acid biosynthesis in a land-crab with advanced terrestrial adaptations: Molecular cloning and functional characterization of two fatty acyl elongases

Ting, Seng Yeat; Lau, Nyok-Sean; Sam, Ka-Kei; Janaranjani, M.; Wong, Swe Cheng; Monroig, Óscar; Quah, Evan S. H.; Ahmad, Amirrudin; Him, Nik Ahmad Irwan Izzauddin Nik; Ram, Annette Jaya; Shu-Chien, Alexander Chong

doi:10.1016/j.cbpb.2022.110773

Cited by 4 publications

(1 citation statement)

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“…fatty acids in aquatic ecosystems, as well as their commercial interest in aquaculture [34][35][36][37][38][39]. Current evidence suggests that both ωx and Fed enzymes occur in certain copepods [12,37,40], but are absent in other crustaceans [10].…”

Section: Introductionmentioning

confidence: 99%

Examination of gammarid transcriptomes reveals a widespread occurrence of key metabolic genes from epibiont bdelloid rotifers in freshwater species

Ribes-Navarro,

Kabeya,

Castro

et al. 2023

Open Biol.

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Previous data revealed the unexpected presence of genes encoding for long-chain polyunsaturated fatty acid (LC-PUFA) biosynthetic enzymes in transcriptomes from freshwater gammarids but not in marine species, even though closely related species were compared. This study aimed to clarify the origin and occurrence of selected LC-PUFA biosynthesis gene markers across all published gammarid transcriptomes. Through systematic searches, we confirmed the widespread occurrence of sequences from seven elongases and desaturases involved in LC-PUFA biosynthesis, in transcriptomes from freshwater gammarids but not marine species, and clarified that such occurrence is independent from the gammarid species and geographical origin. The phylogenetic analysis established that the retrieved elongase and desaturase sequences were closely related to bdelloid rotifers, confirming that multiple transcriptomes from freshwater gammarids contain contaminating rotifers' genetic material. Using the Adineta steineri genome, we investigated the genomic location and exon–intron organization of the elongase and desaturase genes, establishing they are all genome-anchored and, importantly, identifying instances of horizontal gene transfer. Finally, we provide compelling evidence demonstrating Bdelloidea desaturases and elongases enable these organisms to perform all the reactions for de novo biosynthesis of PUFA and, from them, LC-PUFA, an advantageous trait when considering the low abundance of these essential nutrients in freshwater environments.

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