Long non-coding RNA AFAP1-AS1 plays an oncogenic role in promoting cell migration in non-small cell lung cancer

He, Jinzhi; Wu, Ke; Guo, Chenglin; Zhou, Jian‐Kang; Pu, Wenchen; Deng, Yulan; Zuo, Yuanli; Zhao, Yun; Liu, Lunxu; Wei, Yuquan; Peng, Yong

doi:10.1007/s00018-018-2923-8

Cited by 44 publications

(27 citation statements)

References 41 publications

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“…[29][30][31][32][33] Accordingly, lncRNAs are increasing being identified as crucial regulators in human tumors. Among these, antisense lncRNA is emerging as a vital participant with respect to affecting various cancerous developments, [34][35][36][37][38] In the present study, five candidates (DICER1-AS1, FAM83H-AS1, GPC3-AS1, DLX6-AS1 and SOX21-AS1) were subjected to qRT-PCR. [42][43][44][45][46] GPC3-AS1 was found to be significantly up-regulated in all four CC cell lines compared to the normal cell CRL-2614.…”

Section: Discussionmentioning

confidence: 99%

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ELK1‐activated GPC3‐AS1/GPC3 axis promotes the proliferation and migration of cervical cancer cells

Zhu

2019

The Journal of Gene Medicine

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Background Long non‐coding RNA (lncRNA) is reported to be involved in multiple biological processes in numerous human tumors. Furthermore, an increasing number of studies have confirmed the involvement of lncRNA in the initiation and development of human cancers, including cervical cancer (CC). Methods The present study explored the potential role of lncRNA glypican 3 antisense transcript 1 (GPC3‐AS1) with respect to regulating CC cell proliferation and migration. Results A quantitative reverse transcriptase‐polymerase chain reaction confirmed that GPC3‐AS1 was up‐regulated in CC cells compared to normal CRL‐2614 cells. Loss‐of‐function assays demonstrated the negative effect of GPC3‐AS1 depletion on CC cell proliferation and migration. GPC3‐AS1 positively regulated its nearby gene glypican 3 (GPC3). Significant up‐regulation of GPC3 was also observed in CC cells, consistently with GPC3‐AS1. In addition, GPC3‐AS1 and GPC3 synergistically promoted the proliferative and migratory abilities of CC cells. Mechanistic investigation showed that ELK1 acted as the transcription activator of GPC3‐AS1 and GPC3, thus contributing to their up‐regulation in CC cells. Rescue assays indicated that the ELK1‐induced GPC3‐AS1/GPC3 axis promoted cell proliferation and migration in CC. Conclusions The results of the present study have revealed a novel molecular pathway that can regulate CC cell proliferation and migration, thus providing a new basis for investigating the molecular mechanism associated with CC progression.

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Section: Discussionmentioning

confidence: 99%

“…Accordingly, lncRNAs are increasing being identified as crucial regulators in human tumors. Among these, antisense lncRNA is emerging as a vital participant with respect to affecting various cancerous developments, including CC. Rui et al .…”

Section: Discussionmentioning

confidence: 99%

ELK1‐activated GPC3‐AS1/GPC3 axis promotes the proliferation and migration of cervical cancer cells

Zhu

2019

The Journal of Gene Medicine

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“…UCA1 upregulates ERBB4 expression through competitively sponging miR-193a-3p, thus enhancing the proliferation of NSCLC cells 18 . AFAP1-AS1 promotes NSCLC cell migration and invasion through positive regulation of AFAP1 protein expression 19 . In LKB1-inactivated NSCLC cells, LINC00473 interacts with NONO, a component of the cAMP signaling pathway to facilitate CRTC/CREB-mediated transcription.…”

Section: Discussionmentioning

confidence: 99%

Exosome-transmitted lncRNA UFC1 promotes non-small-cell lung cancer progression by EZH2-mediated epigenetic silencing of PTEN expression

et al. 2020

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Long non-coding RNAs (LncRNAs) have been suggested as important regulators of cancer development and progression in non-small cell lung cancer (NSCLC). Nevertheless, the biological roles and clinical significance of lncRNA UFC1 in NSCLC remain unclear. We detected the expression of UFC1 in tumor tissues, serum, and serum exosomes of NSCLC patients by qRT-PCR. Gene overexpression or silencing were used to examine the biological roles of UFC1 in NSCLC. RNA immunoprecipitation and ChIP assays were performed to evaluate the interaction between UFC1 and enhancer of zeste homolog 2 (EZH2) and the binding of EZH2 to PTEN gene promoter. Rescue study was used to access the importance of PTEN regulation by UFC1 in NSCLC progression. UFC1 expression was upregulated in tumor tissues, serum, and serum exosomes of NSCLC patients and high level of UFC1 was associated with tumor infiltration. UFC1 knockdown inhibited NSCLC cell proliferation, migration and invasion while promoted cell cycle arrest and apoptosis. UFC1 overexpression led to the opposite effects. Mechanistically, UFC1 bound to EZH2 and mediated its accumulation at the promoter region of PTEN gene, resulting in the trimethylation of H3K27 and the inhibition of PTEN expression. UFC1 knockdown inhibited NSCLC growth in mouse xenograft tumor models while the simultaneous depletion of PTEN reversed this effect. NSCLC cells derived exosomes could promote NSCLC cell proliferation, migration and invasion through the transfer of UFC1. Moreover, Exosome-transmitted UFC1 promotes NSCLC progression by inhibiting PTEN expression via EZH2-mediated epigenetic silencing. Exosome-mediated transmit of UFC1 may represent a new mechanism for NSCLC progression and provide a potential marker for NSCLC diagnosis.

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“…lncRNA TUBA4B has been reported that it may serves as a newly predictor for prognosis and modulate the cell viability in non-small cell lung cancer [34]. lncRNA AFAP1-AS1 may acts as an oncogenic to facilitate the migration of non-small cell lung cancer (NSCLC) [35]. LncRNA SNHG1 may acts as an oncogene through ZEB1 signaling pathway by suppressing Tap63 in LUSC [36].…”

Section: Discussionmentioning

confidence: 99%

Identification of lncRNA biomarkers in lung squamous cell carcinoma using comprehensive analysis of lncRNA mediated ceRNA network

Yang

Jin

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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2019) Identification of lncRNA biomarkers in lung squamous cell carcinoma using comprehensive analysis of lncRNA mediated ceRNA network, Artificial Cells, Nanomedicine, and Biotechnology, 47:1, 3246-3258, ABSTRACTLong non-coding RNAs (lncRNAs) act as a member of competing endogenous RNAs (ceRNAs) and plays a significant role in tumorigenesis. The aim of this study was to identify potential lncRNA biomarkers for predicting the prognosis of lung squamous cell carcinoma (LUSC) using a comprehensive analysis of lncRNA mediated ceRNA network. Differentially expressed RNAs datasets were obtained using edge R package in 502 LUSC tissues and 49 adjacent non-LUSC tissues from the Cancer Genome Atlas (TCGA). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were performed to identify functional enrichment implication of lncRNA related differentially expressed mRNAs. Survival analysis was used Kaplan-Meier curve method. Univariate and multivariate Cox regression analysis were performed to construct a predictive model with lncRNA biomarkers. A total of 2185 lncRNAs, 170 miRNAs and 2053 mRNAs were differentially expressed between LUSC tissues and adjacent non-LUSC tissues. The novel constructed ceRNA network incorporated 184 LUSC-specific lncRNAs, 18 miRNAs, and 49 mRNAs. About 11 of 184 differentially expressed lncRNAs and 1 of 18 differentially expressed miRNAs and 5 of 49 differentially expressed mRNAs were conspicuously related to overall survival (p < .05). Univariate and multivariate cox regression analysis showed that 6 lncRNAs were retrieved to construct a predictive model to predict the overall survival in LUSC patients. In conclusion, CeRNAs contributed to the progression of LUSC and a model with 6 lncRNAs might be potential biomarker for predicting the prognosis of LUSC. ARTICLE HISTORY

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Long non-coding RNA AFAP1-AS1 plays an oncogenic role in promoting cell migration in non-small cell lung cancer

Cited by 44 publications

References 41 publications

ELK1‐activated GPC3‐AS1/GPC3 axis promotes the proliferation and migration of cervical cancer cells

ELK1‐activated GPC3‐AS1/GPC3 axis promotes the proliferation and migration of cervical cancer cells

Exosome-transmitted lncRNA UFC1 promotes non-small-cell lung cancer progression by EZH2-mediated epigenetic silencing of PTEN expression

Identification of lncRNA biomarkers in lung squamous cell carcinoma using comprehensive analysis of lncRNA mediated ceRNA network

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