Long non-coding RNA <i>FEZF1-AS1</i> facilitates cell proliferation and migration in colorectal carcinoma

Chen, Na; Guo, Dan; Xu, Qiong; Yang, Mei; Wang, Dan; Peng, Man; Ding, Yan‐Qing; Wang, Shuang; Zhou, Jun

doi:10.18632/oncotarget.7168

Cited by 95 publications

(112 citation statements)

References 36 publications

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“…Among the eligible articles, 31 were from China, 3 from Japan and 1 from Czech Republic. In the including 34 studies, 29 articles analyzed the relationship between the expression of lncRNAs and gender[16–20, 24–29, 31, 32, 34–37, 39–46, 48–51], 7 studies estimated the association between lncRNAs and tumor size[19, 24, 25, 27, 41, 42, 48], 16 studies estimated the association between lncRNAs and tumor differentiation[16, 17, 19, 24, 25, 28, 29, 34, 36, 37, 39, 40, 43, 45, 46, 48], 24 studies were about lymph node metastasis(LNM)[16–20, 26, 27, 29–32, 34–37, 39, 40, 42–46, 48, 50], and 20 were about distant metastasis[16, 18–20, 25, 27–29, 35–37, 39, 40, 42–46, 48, 50], 18 studies demonstrated that lncRNAs were correlated with TNM stage[…”

Section: Resultsmentioning

confidence: 99%

The prognostic value of abnormally expressed lncRNAs in colorectal cancer: A meta-analysis

Wang

et al. 2017

PLoS ONE

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BackgroundColorectal cancer (CRC) is the third most prevalent cancer type and the third leading cause of cancer-related deaths worldwide, it is urgently needed to discover a new marker for the progress of CRC. Many long noncoding RNAs (lncRNAs) have been reported to be abnormally expressed in CRC, and may be feasible as effective biomarkers and prognostic factors. The aim of this study was to identify the prognostic value of various lncRNAs in CRC.MethodsPubmed, Web of Science, Embase and Cochrane Library were searched for potentially related studies. A total of 34 eligible studies including 30 on overall survival (OS), 7 on disease-free survival (DFS), 1 on relapse-free survival (RFS), 2 on disease-specific survival (DSS) and 29 on clinicopathological features were qualified from the databases.ResultsThe results showed that the expression levels of lncRNAs were significantly associated with poor OS (hazard ratio (HR) = 2.08, 95% confidence interval (CI) = 1.68–2.57, P<0.001, I2 = 70%), DFS (HR = 1.79, 95% CI = 1.54–2.08, P<0.001, I2 = 6%) and DSS (HR = 0.11, 95% CI = 0.02–0.54, P = 0.007, I2 = 14%). Subgroup analysis further showed that lncRNA transcription level was significantly associated with tumor differentiation (odds ratio (OR) = 0.51, 95% CI = 0.34–0.77, P = 0.001), lymph node metastasis (OR = 1.63, 95% CI = 1.23–2.17, P = 0.0007), distant metastasis (OR = 2.06, 95% CI = 1.29–3.30, P = 0.002), TNM stage (OR = 0.44, 95% CI = 0.32–0.62, P<0.001), tumor invasion depth (OR = 0.48, 95% CI = 0.39–0.60, P<0.001).ConclusionsThe meta-analysis demonstrated that abnormal lncRNA transcription level may serve as a promising indicator for prognostic of patients with CRC.

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Section: Resultsmentioning

confidence: 99%

The prognostic value of abnormally expressed lncRNAs in colorectal cancer: A meta-analysis

Wang

et al. 2017

PLoS ONE

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“…C, The translational levels of apoptosis relative markers a tremendous amount of work has been done studying lncRNAs, and many of these lncRNAs have been shown to play important roles in the progression of CRC cells 20,21. A, FACS analysis detected the apoptosis cells proportion after upregulating lncRNA RUNX1-IT1.…”

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confidence: 99%

Long non‐coding RNA RUNX1‐IT1 plays a tumour‐suppressive role in colorectal cancer by inhibiting cell proliferation and migration

Shi

Zhong

Song

et al. 2018

Cell Biochemistry & Function

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Long non-coding RNAs (lncRNAs) have been demonstrated to be involved in the progression of various cancers. In this study, we aim to investigate the role of lncRNA RUNX1-IT1 in the development of colorectal cancer (CRC). The expression levels of lncRNA RUNX1-IT1 were measured using quantitative real-time Polymerase Chain Reaction(qRT-PCR). CCK8 proliferation assay, transwell assay, and flow cytometry were performed to evaluate the effect of lncRNA RUNX1-IT1 on CRC cell proliferation, migration, and apoptosis. The proliferation markers (PCNA, Ki67), apoptosis markers (cleaved-PARP, cleaved-caspase3), and MMP9 are detected by western blotting. Significant down regulation of lncRNA RUNX1-IT1 was measured in CRC tissues and three CRC cell lines (HCT116, HT29, and RKO) compared with paired nontumorous adjacent tissues (P < 0.01) or the normal colonic epithelial cell line FHC (P < 0.05), respectively. Moreover, the proliferative and migration potential of CRC cells were inhibited by overexpressing lncRNA RUNX1-IT1, which could be obviously improved by knocking down lncRNA RUNX1-IT1. The protein levels of PCNA, Ki67, and MMP9 were upregulated by overexpressing lncRNA RUNX1-IT1 and down regulated in si-RUNX1-IT1 cells.Besides, lncRNA RUNX1-IT1 could also promote the apoptosis of CRC cells. In conclusion, lncRNA RUNX1-IT1 is downregulated in CRC and plays a tumour-suppressive role due to the regulatory of cell proliferation, migration, and apoptosis. Significance of the study:We demonstrated that lncRNA RUNX1-IT1 was down regulated both in CRC tissues and cell lines. Besides, lncRNA RUNX1-IT1 could serve as a potential diagnostic biomarker and play a tumour-suppressive role owing to its good diagnostic efficacy and inhibition of CRC cell proliferation and migration.

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“…Moreover, DANCR could be considered as an independent prognosticator of OS with HR, 2.131; 95% CI, 1.157‐7.058; and P = 0.009, and disease‐free survival (DFS) with HR, 2.397; 95% CI, 1.385‐7.279; and P = 0.006 in CRCs . FEZF1‐AS1 is the other lncRNA which correlates with CRC metastasis, including T stage ( P = 0.005), N stage ( P = 0.004) and M stage ( P = 0.020) . It has been shown that FEZF1‐AS1 overexpressed patients have lower survival time than those with normal FEZF1‐AS1 status (53.53 days ± 3.86 vs. 79.40 days ± 4.02).…”

Section: Discussionmentioning

confidence: 99%

Investigating the diagnostic performance of HOTTIP, PVT1, and UCA1 long noncoding RNAs as a predictive panel for the screening of colorectal cancer patients with lymph node metastasis

Gharib

Anaraki

Baghdar

et al. 2019

J of Cellular Biochemistry

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Like other noncoding RNAs (ncRNAs), dysregulation of long ncRNAs (lncRNAs) has been associated with various clinicopathological features of colorectal cancer (CRC) patients such as lymph node metastasis (LNM). Recently, three aberrant expressed oncogenic lncRNA (onco‐lncRNAs), including HOXA transcript at the distal tip (HOTTIP), plasmacytoma variant translocation 1 (PVT1), and urothelial carcinoma associated 1 (UCA1) have been reported in LNM. Herein, we compared the diagnostic performance of these lncRNAs as individual biomarkers and as a discriminating panel between LNM CRC patients, nonmetastatic lymph nodes (NLN) and normal healthy subjects. The lncRNAs expression level was measured by quantitative real‐time PCR and analyzed by the Mann‐Whitney U test. The receiver operating characteristic (ROC) curve analysis was applied to evaluate the diagnostic power. The Kaplan‐Meier survival analysis was performed to outline the overall survival (OS) of CRC patients with an abnormal level of lncRNAs. The area under the ROC curve (AUC) of the overexpressed HOTTIP (0.7817; 95% CI, 0.6809‐0.8824), PVT1 (0.8559; 95% CI, 0.7737‐0.9382), and UCA1 (0.8135; 95% CI, 0.722‐0.9051) introduced them as individual CRC biomarkers. As a predictive panel, the AUC values of the HOTTIP, PVT1, and UCA1 for training set were 0.9256 (95% CI, 0.8634‐0.9879; all CRCs), 0.8708 (95% CI, 0.7709‐0.9378; NLN) and 0.9804 (95% CI, 0.9585‐0.9998; LNM), and for validation set were 0.9286 (95% CI, 0.8752‐0.9820; all CRCs), 0.8911 (95% CI, 0.8238‐0.9585; NLN), and 0.9833 (95% CI, 0.9642‐1.002; LNM), respectively. Also, HOTTIP/PVT1/UCA1 panel dysregulation had a marked correlation with patient's OS in training set (logrank test P = 0.0121; hazard ratio [HR], 0.1225; 95% confidence interval [CI], 0.02376‐0.6312), and in validation set (logrank test P < 0.0001, HR, 0.2003; 95% CI, 0.08942‐0.4486). These data showed that the combination of HOTTIP, PVT1, and UCA1 as a predictive panel, has a better diagnostic performance than each of these lncRNAs individually, and could be used for the screening of patients with advanced CRC.

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Long non-coding RNA FEZF1-AS1 facilitates cell proliferation and migration in colorectal carcinoma

Cited by 95 publications

References 36 publications

The prognostic value of abnormally expressed lncRNAs in colorectal cancer: A meta-analysis

The prognostic value of abnormally expressed lncRNAs in colorectal cancer: A meta-analysis

Long non‐coding RNA RUNX1‐IT1 plays a tumour‐suppressive role in colorectal cancer by inhibiting cell proliferation and migration

Investigating the diagnostic performance of HOTTIP, PVT1, and UCA1 long noncoding RNAs as a predictive panel for the screening of colorectal cancer patients with lymph node metastasis

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