Long non-coding RNA RAB11B-AS1 prevents osteosarcoma development and progression via its natural antisense transcript RAB11B

Abstract

Long non-coding RNAs (lncRNAs) have been shown to exert essential roles in development and progression of tumors. Here we discovered a novel lncRNA, RAB11B antisense RNA (RAB11B-AS1), which is markedly down-regulated in human osteosarcoma (OS) and associated with OS metastasis and poor prognosis. We find that reduction of RAB11B-AS1 significantly facilitates proliferation, migration and invasiveness and prevents apoptosis of OS cells and results in lower sensitivity to cisplatin in these cells. In contrast, up-regulation of RAB11B-AS1 suppresses the aggressive behaviors of OS cells. Mechanistically, down-regulation of RAB11B-AS1 elevates its sense-cognate gene RAB11B expression at both mRNA and protein levels. RAB11B-AS1 expression correlates negatively with RAB11B expression in OS tissues. Luciferase reporter assay illuminated that RAB11B-AS1 regulates RAB11B expression through antisense pairing. Most importantly, all the effects of RAB11B-AS1 were abrogated by RAB11B down-regulation. Thus our findings revealed that lnc-RAB11B-AS1 prevents osteosarcoma development and progression via inhibiting RAB11B expression, indicating lnc-RAB11B-AS1 as a potential therapeutic target for osteosarcoma.

Long noncoding RNA SLC2A1‐AS1 regulates aerobic glycolysis and progression in hepatocellular carcinoma via inhibiting the STAT3/FOXM1/GLUT1 pathway

“…C). The ‘head‐to‐head’ or ‘head‐to‐tail’ complementary pairing pattern has been shown to play a crucial role in the regulation of antisense lncRNAs and their opposite strand coding genes (Chen et al , 2018; Li et al , 2012). Hence, we constructed two plasmids containing the complementary pairing sequence of SLC2A1‐AS1 (pSLC2A1‐AS1‐part1) and the noncomplementary sequence of SLC2A1‐AS1 (pSLC2A1‐AS1‐part2) separately for cell transfection.…”

LncRNA NR-104098 Inhibits AML Proliferation and Induces Differentiation Through Repressing EZH2 Transcription by Interacting With E2F1

“…LncRNA is a non-coding RNA longer than 200 bp that does not have the ability to encode proteins (Chen et al, 2018). LncRNA can be classified into different types, such as sense, antisense, intronic, intergenic, and bidirectional (Ma et al, 2012).…”

<p>lncRNA FLVCR-AS1 promotes osteosarcoma growth by targeting miR381-3p/CCND1</p>

“…According to the existing literature, GAS5, MEG3, RAB11B-AS1, and WWOX-AS1 are considered tumor suppressors in OS, and can inhibit OS development by suppressing OS-cell proliferation and epithelial-mesenchymal transition. [19][20][21][22] On the contrary, some other lncRNAs, such as DANCR, C2dat1, XIST, SATB2-AS1, ITGB2-AS1, MALAT1, HULC, CCAL, CBR3-AS1, ZEB1-AS1, and FGFR3-AS1, can promote OS progression by enhancing OS-cells proliferation, migration, and invasion and weakening OS-cells apoptosi.…”