Long non-coding RNA RP11-490M8.1 inhibits lipopolysaccharide-induced pyroptosis of human umbilical vein endothelial cells via the TLR4/NF-κB pathway

Li, Xuehui; Wu, Limei; Wang, Jiali; Dong, Xian‐Hui; Zhang, Shun-Chi; Li, Xue-Heng; Xu, Hui; Liu, Da-Bin; Li, Zhihai; Liu, Zheming; Wu, Shao-Guo; Hu, Yan‐Wei

doi:10.1016/j.imbio.2021.152133

Cited by 12 publications

(6 citation statements)

References 48 publications

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“…In Fig. 4 , various study demonstrated that LPS could increase IL-1β expression, caspase-1 expression, cell migration and proliferation compared to the no LPS control ( 18 , 22 , 39 , 40 ). Thus, ‘no LPS’ was not used.…”

Section: Discussionmentioning

confidence: 90%

“…A previous study has shown that LPS promoted the progression and metastasis of cervical cancer by stimulating the release of IL-1β; thus, promoting downstream cytokines and pyroptosis ( 14 ). LPS markedly induced pyroptosis by increasing the protein levels of caspase-1 ( 22 ). Metformin inhibits cancer cell proliferation, migration and invasion ( 23 ).…”

Section: Resultsmentioning

confidence: 99%

“…LPS stimulation activated the TLR4-NF-κB signaling pathway and inflammatory mediators, such as IL-1β, in cervical squamous carcinoma cells ( 18 ). LPS markedly induced pyroptosis by increasing the protein levels of caspase-1 ( 22 ). Cell migration and proliferation were also promoted by LPS in cervical cancer SiHa cells ( 39 ).…”

Section: Discussionmentioning

confidence: 99%

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Effects of Forkhead box O1 on lipopolysaccharide‑induced mitochondrial dysfunction in human cervical squamous carcinoma SiHa cells

Wang

Gao

et al. 2021

Oncol Lett

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Persistent infection and chronic inflammation play important roles in the development of cervical squamous cell carcinoma. Forkhead box O1 (FOXO1) is a notable regulator of mitochondrial metabolism, which is involved in the occurrence and development of tumors. The present study explored the effects of FOXO1 in human cervical squamous carcinoma SiHa cells. The expression of FOXO1 was examined using reverse transcription-quantitative PCR, western blotting and immunohistochemical staining. SiHa cell migration and proliferation were detected using Transwell and 3 H-TdR assays. Mitochondrial functions were assessed based on reactive oxygen species (ROS) generation and changes in the mitochondrial membrane potential ( ΔΨm ). The present study revealed that lipopolysaccharide (LPS) stimulation significantly inhibited the expression of FOXO1 in cervical squamous carcinoma SiHa cells; while silencing FOXO1 resulted in the accumulation of mitochondrial ROS, a decrease in the ΔΨm and abnormal morphology of mitochondria. Accordingly, enhancing FOXO1 expression or treatment with metformin, which protects mitochondrial function, reversed LPS-induced mitochondrial dysfunction, cell pyroptosis, migration and proliferation of cervical squamous carcinoma SiHa cells. Overall, the current study indicated that treatment with FOXO1 could potentially be used as therapeutic strategy to prevent LPS-induced cervical squamous cell carcinoma-related dysfunction in a mitochondria-dependent manner.

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Section: Discussionmentioning

confidence: 90%

Section: Resultsmentioning

confidence: 99%

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Effects of Forkhead box O1 on lipopolysaccharide‑induced mitochondrial dysfunction in human cervical squamous carcinoma SiHa cells

Wang

Gao

et al. 2021

Oncol Lett

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“…Additionally, LPS can activate TLR4 and initiate the innate immune and inflammatory response through the activation of NF-κB and MAPK in mammary epithelial cells (54,55), which induce the expression of pro-inflammatory cytokines IL-6 and TNF-α (56). Liu et al (57) showed that lncRNA RP11-490M8.1 could reduce LPS-induced pyroptosis via TLR4/NF-κB signaling pathway. Furthermore, Zhao et al (58) found that lncRNA UCA1 could accelerate cell viability, block apoptosis and function as competitive endogenous RNA (ceRNA) to target TLR4 by sponging miR-499b-5p, thereby alleviating the LPS-challenged cell damage.…”

Section: Discussionmentioning

confidence: 99%

Differential Expression Profiles of lncRNA Following LPS-Induced Inflammation in Bovine Mammary Epithelial Cells

Wang

Yang

et al. 2021

Front. Vet. Sci.

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Bovine mastitis is an inflammatory response of mammary glands caused by pathogenic microorganisms such as Escherichia coli (E. coli). As a key virulence factor of E. coli, lipopolysaccharide (LPS) triggers innate immune responses via activation of the toll-like-receptor 4 (TLR4) signaling pathway. However, the molecular regulatory network of LPS-induced bovine mastitis has yet to be fully mapped. In this study, bovine mammary epithelial cell lines MAC-T were exposed to LPS for 0, 6 and 12 h to assess the expression profiles of long non-coding RNAs (lncRNAs) using RNA-seq. Differentially expressed lncRNAs (DElncRNAs) were filtered out of the raw data for subsequent analyses. A total of 2,257 lncRNAs, including 210 annotated and 2047 novel lncRNAs were detected in all samples. A large proportion of lncRNAs were present in a high abundance, and 112 DElncRNAs were screened out at different time points. Compared with 0 h, there were 22 up- and 25 down-regulated lncRNAs in the 6 h of post-infection (hpi) group, and 27 up- and 22 down-regulated lncRNAs in the 12 hpi group. Compared with the 6 hpi group, 32 lncRNAs were up-regulated and 25 lncRNAs were down-regulated in the 12 hpi group. These DElncRNAs are involved in the regulation of a variety of immune-related processes including inflammatory responses bMECs exposed to LPS. Furthermore, lncRNA TCONS_00039271 and TCONS_00139850 were respectively significance down- and up-regulated, and their target genes involve in regulating inflammation-related signaling pathways (i.e.,Notch, NF-κB, MAPK, PI3K-Akt and mTOR signaling pathway), thereby regulating the occurrence and development of E. coli mastitis. This study provides a resource for lncRNA research on the molecular regulation of bovine mastitis

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“…However, the research on lncRNA NEXN-AS1-mediated pyroptosis is still unclear, and it is worth exploring the underlying molecular mechanism ( Wu et al, 2020 ). In another probe on AS ( Liu X.-H. et al, 2021 ), researchers discovered that lncRNA RP11-490M8.1 blocked LPS-induced pyroptosis of Human Umbilical Vein Endothelial Cells (HUVECs) through the TLR4/NF-κb signal axis, implying that lncRNA RP11-490M8.1 may act as a therapeutic target for AS. Nevertheless, this work did not explore which gasdermin protein ultimately affected by lncRNA RP11-490M8.1, and did not identify it in-depth in animal models.…”

Section: Non-coding Rna and Pyroptosismentioning

confidence: 99%

Regulation of Pyroptosis by ncRNA: A Novel Research Direction

Gao

Jiang

Han

et al. 2022

Front. Cell Dev. Biol.

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Pyroptosis is a novel form of programmed cell death (PCD), which is characterized by DNA fragmentation, chromatin condensation, cell swelling and leakage of cell contents. The process of pyroptosis is performed by certain inflammasome and executor gasdermin family member. Previous researches have manifested that pyroptosis is closely related to human diseases (such as inflammatory diseases) and malignant tumors, while the regulation mechanism of pyroptosis is not yet clear. Non-coding RNA (ncRNA) such as microRNA (miRNA), long non-coding RNA (lncRNA) and circular RNA (circRNA) have been widely identified in the genome of eukaryotes and played a paramount role in the development of cell function and fate after transcription. Accumulating evidences support the importance of ncRNA biology in the hallmarks of pyroptosis. However, the associations between ncRNA and pyroptosis are rarely reviewed. In this review, we are trying to summarize the regulation and function of ncRNA in cell pyroptosis, which provides a new research direction and ideas for the study of pyroptosis in different diseases.

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Long non-coding RNA RP11-490M8.1 inhibits lipopolysaccharide-induced pyroptosis of human umbilical vein endothelial cells via the TLR4/NF-κB pathway

Cited by 12 publications

References 48 publications

Effects of Forkhead box O1 on lipopolysaccharide‑induced mitochondrial dysfunction in human cervical squamous carcinoma SiHa cells

Effects of Forkhead box O1 on lipopolysaccharide‑induced mitochondrial dysfunction in human cervical squamous carcinoma SiHa cells

Differential Expression Profiles of lncRNA Following LPS-Induced Inflammation in Bovine Mammary Epithelial Cells

Regulation of Pyroptosis by ncRNA: A Novel Research Direction

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