Long non-coding RNA SPRY4-IT1 promotes the proliferation and invasion of U251 cells through upregulation of SKA2

He, Xiaoxi; Bian, Erbao; Ma, Chunchun; Wang, Chao; Wang, Hongliang; Zhao, Bing

doi:10.3892/ol.2018.7776

Cited by 9 publications

(11 citation statements)

References 36 publications

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“…Consistently, two independent studies reported decreased mRNA levels of BDNF and TrkB in the prefrontal cortex and hippocampus of individuals who committed suicide as compared to healthy control subjects [18,19]. Moreover, SPRY4-IT1, a long non-coding RNA derived from the second intron of SPRY4 [20], has been shown to interact with SKA2 [21], a gene that was suggested to be a promising biomarker for suicidal behavior [22,23], stress susceptibility, and stress-related disorders such as PTSD [23][24][25]. Finally, SPRY4 was previously found differentially methylated in the blood of patients diagnosed with schizophrenia [26].…”

Section: Discussionmentioning

confidence: 62%

Longitudinal epigenome-wide association studies of three male military cohorts reveal multiple CpG sites associated with post-traumatic stress disorder

et al. 2020

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Background: Epigenetic mechanisms have been suggested to play a role in the development of post-traumatic stress disorder (PTSD). Here, blood-derived DNA methylation data (HumanMethylation450 BeadChip) collected prior to and following combat exposure in three cohorts of male military members were analyzed to assess whether DNA methylation profiles are associated with the development of PTSD. A total of 123 PTSD cases and 143 traumaexposed controls were included in the analyses. The Psychiatric Genomics Consortium (PGC) PTSD EWAS QC pipeline was used on all cohorts, and results were combined using a sample size weighted meta-analysis in a twostage design. In stage one, we jointly analyzed data of two new cohorts (N = 126 and 78) for gene discovery, and sought to replicate significant findings in a third, previously published cohort (N = 62) to assess the robustness of our results. In stage 2, we aimed at maximizing power for gene discovery by combining all three cohorts in a metaanalysis. Results: Stage 1 analyses identified four CpG sites in which, conditional on pre-deployment DNA methylation, postdeployment DNA methylation was significantly associated with PTSD status after epigenome-wide adjustment for multiple comparisons. The most significant (intergenic) CpG cg05656210 (p = 1.0 × 10 −08) was located on 5q31 and significantly replicated in the third cohort. In addition, 19 differentially methylated regions (DMRs) were identified, but failed replication. Stage 2 analyses identified three epigenome-wide significant CpGs, the intergenic CpG cg05656210 and two additional CpGs located in MAD1L1 (cg12169700) and HEXDC (cg20756026). Interestingly, cg12169700 had an underlying single nucleotide polymorphism (SNP) which was located within the same LD block as a recently identified PTSD-associated SNP in MAD1L1. Stage 2 analyses further identified 12 significant differential methylated regions (DMRs), 1 of which was located in MAD1L1 and 4 were situated in the human leukocyte antigen (HLA) region.

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Section: Discussionmentioning

confidence: 62%

Longitudinal epigenome-wide association studies of three male military cohorts reveal multiple CpG sites associated with post-traumatic stress disorder

et al. 2020

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“…Therefore, exploring new regulating cancer genes and finding the new regulatory mechanism has become the key points in the field of HCC. Emerging evidence has shown that SKA2 was involved in tumorigenesis of various cancers, including breast cancer, lung cancer, and glioma [13][14][15][16].…”

Section: Discussionmentioning

confidence: 99%

“…Recent evidences showed that overexpression of SKA2 promoted proliferation of human breast cancer, whereas SKA2 knockdown in human lung epithelial cells reduced transactivation and suppressed dexamethasone inhibition of proliferations [11,12]. Furthermore, the function of SKA2 has been observed in various cancers, including breast cancer, lung cancer, and glioma [13][14][15][16]. However, the role of SKA2 in HCC still remained unclear.…”

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confidence: 99%

SKA2 promotes proliferation and invasion of hepatocellular carcinoma cells via activating the β-catenin signaling pathway

Wang¹,

Suo²,

Gong³

et al. 2020

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Spindle and kinetochore associated complex subunit 2 (SKA2) is a part of the spindle and kinetochore associated (SKA) complex, which has been reported in various cancers, including the breast cancer, lung cancer, and glioma. However, its role remains unclear in hepatocellular carcinoma (HCC). Our study found that SKA2 mRNA levels and immunohistochemistry staining were significantly increased in HCC tissues, compared with normal tissues. The role of SKA2 in cell proliferation and invasion was also determined. Overexpression of SKA2 significantly promoted cell proliferation and invasion, while knocking down of SKA2 expression inhibited the growth and invasion of HCC cells. In experiments investigating the underlying mechanism, overexpression of SKA2 may increase the expression levels of total -catenin, and knockdown of SKA2 may decrease the expression levels of total -catenin. Our studies thus suggest that SKA2 may promote proliferation and invasion of hepatocellular carcinoma cells by activating the -catenin signaling pathway, which can serve as a potential target in the diagnosis and/or treatment of HCC.

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“…cg05656210 is an intergenic site annotated near SPRY4. SPRY4 was previously found differentially methylated in blood of patients diagnosed with schizophrenia [29] and has further been shown to interact with SKA2 [30], a gene suggested to be a promising biomarker for suicidal behavior [31,32], stress susceptibility and stress-related disorders such as PTSD [32][33][34].…”

Section: Discussionmentioning

confidence: 99%

Meta-analysis of longitudinal epigenome-wide association studies of military cohorts reveals multiple CpG sites associated with post-traumatic stress disorder

Snijders

Maihofer

Ratanatharathorn

et al. 2019

Preprint

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Background: Epigenetic mechanisms have been suggested to play a role in the development of post-traumatic stress disorder (PTSD). Here, blood-derived DNA methylation data (HumanMethylation450 BeadChip) collected prior to and following combat exposure in three cohorts composed of male military members were combined to assess whether DNA methylation profiles are associated with the development of PTSD. Methods: A total of 123 cases and 143 trauma-exposed controls were included. The Psychiatric Genomics Consortium (PGC) PTSD EWAS QC pipeline was used on all cohorts, and results were combined using a sample size weighted meta-analysis. We first combined two cohorts in a discovery stage (N=126 and 78), sought targeted replication in the third cohort (N=62) and then performed a meta-analysis of all three datasets. Results: The discovery stage identified four CpG sites in which, conditional on pre-deployment DNA methylation, post-deployment DNA methylation was associated with PTSD status after adjustment for multiple comparisons. The most significant CpG (p = 1.0 x 10 -08 ) was located on 5q31 and replicated in the third cohort. When combining all cohorts, this intergenic site remained most significant along with two CpGs located in MAD1L1 and HEXDC. Interestingly, the CpG site of MAD1L1 had an underlying single nucleotide polymorphism (SNP) which was located within the same LD block as a recently identified PTSD-associated SNP. Twelve differential methylated regions (DMRs) were also identified, one of which was located in MAD1L1 and four were situated in the human leukocyte antigen (HLA) region. Conclusion: This study suggests that the development of PTSD is associated with distinct methylation patterns in several genomic positions and regions. Our most prominent finding points to the involvement of MAD1L1 which was previously associated with PTSD.

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Long non-coding RNA SPRY4-IT1 promotes the proliferation and invasion of U251 cells through upregulation of SKA2

Cited by 9 publications

References 36 publications

Longitudinal epigenome-wide association studies of three male military cohorts reveal multiple CpG sites associated with post-traumatic stress disorder

Longitudinal epigenome-wide association studies of three male military cohorts reveal multiple CpG sites associated with post-traumatic stress disorder

SKA2 promotes proliferation and invasion of hepatocellular carcinoma cells via activating the β-catenin signaling pathway

Meta-analysis of longitudinal epigenome-wide association studies of military cohorts reveals multiple CpG sites associated with post-traumatic stress disorder

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