SKA2 promotes proliferation and invasion of hepatocellular carcinoma cells via activating the β-catenin signaling pathway

Wang, D; Suo, Yuhong; Gong, Lan; Lv, Shiliang

doi:10.4149/neo_2020_190709n609

Cited by 4 publications

(2 citation statements)

References 18 publications

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“…Dysregulation of SKA2 has also been reported in past studies. Wang et al 35 revealed the apparent upregulation of SKA2 in hepatocellular carcinoma tissues, confirming that SKA2 had promotion effects on tumor cell metastasis. Zhuang et al 36 also uncovered that SKA2 combined with cyclic-AMP response binding protein and contributed to the proliferation of renal cell carcinoma cells and tumor growth in renal cell carcinoma.…”

Section: Discussionmentioning

confidence: 88%

Circ_0001387 regulates SKA2 to accelerate breast cancer progression through miR‐136‐5p

Xiong

Wang

et al. 2023

Thoracic Cancer

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Background: Growing evidence has revealed the critical regulatory role for circular RNAs (circRNAs) in cancer. This study aimed to explore the function of circ_0001387 in breast cancer (BC). Methods: Circ_0001387, miR-136-5p, and spindle and kinetochore-associated protein 2 (SKA2) levels were analyzed by quantitative real-time polymerase chain reaction. Clone formation and 5-ethynyl-2 0 -deoxyuridine assays were used to analyze cell proliferation. Cell apoptosis and cell migration and invasion abilities were analyzed using flow cytometry or transwell assay. Mechanism assay was used to confirm the association between miR-136-5p and circ_0001387 or SKA2. The effect of circ_0001387 on tumor growth in vivo was analyzed by the xenograft mice model. Results: Circ_0001387 and SKA2 were expressed at high levels, whereas miR-136-5p was lowly expressed in BC tissues and cells. Meanwhile, the downregulation of circ_0001387 restrained BC cell progression in vitro and in vivo. Circ_0001387 competitively bound to miR-136-5p to regulate BC cell malignant behaviors. SKA2 was targeted by miR-136-5p, and SKA2 reinstated the suppressive effect of miR-136-5p upregulation in BC cells. Conclusion: Our study indicated that circ_0001387 contributed to BC cell progression through miR-136-5p/SKA2 axis.

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Section: Discussionmentioning

confidence: 88%

Circ_0001387 regulates SKA2 to accelerate breast cancer progression through miR‐136‐5p

Xiong

Wang

et al. 2023

Thoracic Cancer

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“…A recent study reported that the mRNA level and immunohistochemical staining of SKA2 were significantly increased in HCC tissues compared with normal tissues ( 17 ). Due to the direct targeting of miR-140-3p to SKA2, overexpression of SK2 could partially suppress the inhibitory effect of miR-140-3p restoration in breast cancer cells ( 18 ).…”

Section: Discussionmentioning

confidence: 99%

SKA1/2/3 is a biomarker of poor prognosis in human hepatocellular carcinoma

Song

He²,

Ji³

et al. 2022

Front. Oncol.

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BackgroundSpindle and kinetochore-associated complex subunits 1–3 (SKA1–3) stabilize the kinetochore-attached spindle microtubules in metaphase. Due to the dysregulation in multiple cancers, SKA1–3 is considered a predictor for the prognosis of the patients. However, the potential clinical applications of SKA1–3, particularly in hepatocellular carcinoma (HCC) prognosis and progression, have completely unknown yet.MethodsFor the analysis of SKA1–3 expression and applications in clinics in HCC patients, several databases, such as STRING, UALCAN, GEO, and TCGA, were searched. In addition, the underlying mechanisms of SKA for the regulation of HCC occurrence, development, and progression were also explored.ResultsCompared to the normal controls, HCC patients showed dramatically elevated SKA1–3 expression at the mRNA level, and the values of the area under the curve (AUC) were 0.982, 0.887, and 0.973, respectively. Increased SKA1–3 expression levels were associated with the clinical stage, age, body mass index, tumor grade, tissue subtype, and Tp53 mutation status in HCC patients. The analyses of Kyoto Encyclopedia of Genes and Genome (KEGG) and Gene ontology (GO) demonstrated that SKA1–3 are enriched mainly in the Fanconi anemia, homologous recombination, spliceosome, DNA replication, and cell cycle signaling pathways. The hub genes, such as CDK1, CCNB1, CCNA2, TOP2A, BUB1, AURKB, CCNB2, BUB1B, NCAPG, and KIF11, were identified in protein–protein interactions (PPIs). The expression levels of hub genes were increased in HCC patients and predictive of a poor prognosis. Finally, the expression levels of SKA1–3 were determined using the GEO database.ConclusionsSKA1–3 are potential prognostic biomarkers of and targets for HCC. In addition, SKA1–3 may affect HCC prognosis via the Fanconi anemia pathway, homologous recombination, spliceosome, DNA replication, and cell cycle signaling pathway.

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Transcript levels of spindle and kinetochore-associated complex 1/3 as prognostic biomarkers correlated with immune infiltrates in hepatocellular carcinoma

Chen

et al. 2021

Sci Rep

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The spindle and kinetochore-associated protein complex (Ska) is an essential component in chromosome segregation. It comprises three proteins (Ska1, Ska2, and Ska3) with theorized roles in chromosomal instability and tumor development, and its overexpression has been widely reported in a variety of tumors. However, the prognostic significance and immune infiltration of Ska proteins in hepatocellular carcinoma (HCC) are not completely understood. The bioinformatics tools Oncomine, UALCAN, gene expression profiling interactive analysis 2 (GEPIA2), cBioPortal, GeneMANIA, Metascape, and TIMER were used to analyze differential expression, prognostic value, genetic alteration, and immune cell infiltration of the Ska protein complex in HCC patients. We found that the mRNA expression of the Ska complex was markedly upregulated in HCC. High expression of the Ska complex is closely correlated with tumor stage, patient race, tumor grade, and TP53 mutation status. In addition, high expression of the Ska complex was significantly correlated with poor disease-free survival, while the high expression levels of Ska1 and Ska3 were associated with shorter overall survival. The biological functions of the Ska complex in HCC primarily involve the amplification of signals from kinetochores, the mitotic spindle, and (via a MAD2 invasive signal) unattached kinetochores. Furthermore, the expression of the complex was positively correlated with tumor-infiltrating cells. These results may provide new insights into the development of immunotherapeutic targets and prognostic biomarkers for HCC.

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SKA2 promotes proliferation and invasion of hepatocellular carcinoma cells via activating the β-catenin signaling pathway

Cited by 4 publications

References 18 publications

Circ_0001387 regulates SKA2 to accelerate breast cancer progression through miR‐136‐5p

Circ_0001387 regulates SKA2 to accelerate breast cancer progression through miR‐136‐5p

SKA1/2/3 is a biomarker of poor prognosis in human hepatocellular carcinoma

Transcript levels of spindle and kinetochore-associated complex 1/3 as prognostic biomarkers correlated with immune infiltrates in hepatocellular carcinoma

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