Long non-coding RNA VCAN-AS1 promotes the malignant behaviors of breast cancer by regulating the miR-106a-5p-mediated STAT3/HIF-1α pathway

Du, Peng; Luo, Kaifeng; Li, Guoyong; Zhu, Jisheng; Xiao, Qin; Li, Yong; Zhang, Xingjian

doi:10.1080/21655979.2021.1960774

Cited by 16 publications

(8 citation statements)

References 53 publications

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“…Several studies have examined the functions of lncRNAs in BC [ 8 , 11 , 13 ]. For instance, high expression of the VCAN-AS1 lncRNA was detected in BC, which promotes the malignancy of BC by regulating the miR-106a-5p-regulated signal transducer and activator of transcription 3/hypoxia-inducible factor-1alpha pathway [ 37 ]. The BCAR4 lncRNA is overexpressed in BC cells and tissues, which promotes BC cell proliferation by suppressing p16 expression [ 50 ].…”

Section: Discussionmentioning

confidence: 99%

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Long non-coding RNA (LncRNA) CASC9/microRNA(miR)-590–3p/sine oculis homeobox 1 (SIX1)/NF-κB axis promotes proliferation and migration in breast cancer

Chang¹,

Zhang²,

Ye³

et al. 2021

Bioengineered

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Long non-coding RNA (lncRNA)–microRNA–mRNA signaling axes have recently been shown to have a key role in the development of breast cancer (BC). In this study, we investigated how the cancer susceptibility candidate 9 (CASC9) gene affects the cell growth, invasion, migration, and apoptosis of BC cells. The levels of microRNA-590-3p (miR-590-3p), CASC9, and the sine oculis homeobox 1 (SIX1) gene were determined through qRT-PCR. We conducted cell counting kit-8 (CCK-8) assays to assess cell proliferation, transwell assays to detect cell migration/invasion, and flow cytometry to evaluate cell apoptosis. StarBase v2.0 was used to predict interactions between miR-590-3p and SIX1 or CASC9, and dual-luciferase reporter assays were used to verify these predictions. CASC9 protein was overexpressed in BC cells and tissues, while CASC9 knockdown inhibited BC cell growth, invasion, and migration and promoted apoptosis. Additionally, we verified that CASC9 competes for binding with miR-590-3p. Moreover, SIX1 was determined to be a target of miR-590–3p, and SIX1 expression was inhibited by miR-590-3p overexpression. CASC9 enhanced BC development by downregulating miR-590-3p and upregulating SIX1 during the activation of the NF-κB pathway. These data suggest that the CASC9/miR-590-3p/SIX1/NF-κB axis is involved in breast cancer progression, providing insight into the function of CASC9 in breast cancer development.

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Section: Discussionmentioning

confidence: 99%

“…MDA-MB231 and MCF7 cells were cultivated in RPMI 1640 medium containing 10% FBS. Each sample was incubated at 37°C under 5% CO 2 conditions [ 37 ].…”

Section: Methodsmentioning

confidence: 99%

Long non-coding RNA (LncRNA) CASC9/microRNA(miR)-590–3p/sine oculis homeobox 1 (SIX1)/NF-κB axis promotes proliferation and migration in breast cancer

Chang¹,

Zhang²,

Ye³

et al. 2021

Bioengineered

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“…For example, a significant association was observed at 5q14.2 within non-coding RNA VCAN-AS1 for the central nucleus. This gene was reported to participate in the regulation of developing gastric [52] and breast cancer [53]. The lead SNP rs2875238 at 11q24.3 was identified to be associated with corticoamygdaloid transition solely, mapping to the ADAMTS8 gene, which was found to have modifying effects on perineuronal net structures and involved in the formation of neuronal plasticity and long-term memory [48].…”

Section: Discussionmentioning

confidence: 99%

The genetic architecture of human amygdala volumes and their overlap with common brain disorders

et al. 2023

Transl Psychiatry

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The amygdala is a crucial interconnecting structure in the brain that performs several regulatory functions, yet its genetic architectures and involvement in brain disorders remain largely unknown. We carried out the first multivariate genome-wide association study (GWAS) of amygdala subfield volumes in 27,866 UK Biobank individuals. The whole amygdala was segmented into nine nuclei groups using Bayesian amygdala segmentation. The post-GWAS analysis allowed us to identify causal genetic variants in phenotypes at the SNP, locus, and gene levels, as well as genetic overlap with brain health-related traits. We further generalized our GWAS in Adolescent Brain Cognitive Development (ABCD) cohort. The multivariate GWAS identified 98 independent significant variants within 32 genomic loci associated (P < 5 × 10−8) with amygdala volume and its nine nuclei. The univariate GWAS identified significant hits for eight of the ten volumes, tagging 14 independent genomic loci. Overall, 13 of the 14 loci identified in the univariate GWAS were replicated in the multivariate GWAS. The generalization in ABCD cohort supported the GWAS results with the 12q23.2 (RNA gene RP11-210L7.1) being discovered. All of these imaging phenotypes are heritable, with heritability ranging from 15% to 27%. Gene-based analyses revealed pathways relating to cell differentiation/development and ion transporter/homeostasis, with the astrocytes found to be significantly enriched. Pleiotropy analyses revealed shared variants with neurological and psychiatric disorders under the conjFDR threshold of 0.05. These findings advance our understanding of the complex genetic architectures of amygdala and their relevance in neurological and psychiatric disorders.

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“…Meanwhile, HAGLR is highly expressed in GC tissues and cells and was found as a molecular sponge of miR-338-3p to promote 5-Fu resistance in GC via targeting the LDHA-glycolysis pathway [16]. Furthermore, voluminous literature has manifested that VCAN-AS1 and HAGLR also play a regulatory role in the occurrence and development of various tumors except for GC [17][18][19][20]. To our knowledge, CDKN2B-AS1 is abnormally expressed in various tumors [21][22][23].…”

Section: Discussionmentioning

confidence: 99%

Development and Evaluation of a Novel Cuproptosis‐Related lncRNA Signature for Gastric Cancer Prognosis

Yin

Gao

Wang

et al. 2023

Computational and Mathematical Methods in Medicine

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Background. According to a growing body of research, long noncoding RNAs (lncRNAs) participate in the progress of gastric cancer (GC). Cuproptosis is a distinct kind of programmed cell death, separating it from several other forms of programmed cell death that may be caused by genetic programming. Consequently, it is crucial to examine cuproptosis-related lncRNAs (CRLs) prognostic importance for the prognosis and treatment response in GC. Method. The Cancer Genome Atlas (TCGA) database was used to retrieve RNA-seq data, pertinent clinical information, and somatic mutation data. A list of cuproptosis-related genes (CRGs) was obtained from prior work. We can distinguish prognostic CRLs using coexpression and univariate Cox analysis. Then, using CRLs, we developed a risk prediction model using multivariate Cox regression analysis and the least absolute shrinkage selection operator (LASSO) technique. To evaluate the diagnostic accuracy of this model, a Kaplan-Meier (K-M) survival analysis and a receiver operating characteristic (ROC) analysis were used. Moreover, the relationships between the risk model and immunological function, somatic mutation, and drug sensitivity were also investigated. Results. Using the multivariate Cox analysis technique, we developed a signature based on cuproptosis-related four lncRNAs. We then classified patients into high-risk and low-risk groups based on the likelihood of unfavorable outcomes. The model was subjected to further testing, including K-M survival analysis, ROC analysis, and multivariate Cox regression analysis, all of which proved the model’s exceptional robustness and predictive capacity. In addition, a nomogram that has a strong capacity for prediction ability was built. This nomogram included age, gender, clinical grade, pathologic stage, T stage, and risk score. Furthermore, we discovered substantial disparities in immune function and the number of mutations carried by tumors between the high-risk and low-risk groups. Moreover, this research also found that the IC50 values for 27 chemotherapeutic drugs varied widely across patients within high- and low-risk groups. Conclusion. The proposed 4-CRLs signature is a promising biomarker to predict clinical outcomes in GC.

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Long non-coding RNA VCAN-AS1 promotes the malignant behaviors of breast cancer by regulating the miR-106a-5p-mediated STAT3/HIF-1α pathway

Abstract: Zhang (2021) Long non-coding RNA VCAN-AS1 promotes the malignant behaviors of breast cancer by regulating the miR-106a-5p-mediated STAT3/HIF-1α pathway,

Cited by 16 publications

References 53 publications

Long non-coding RNA (LncRNA) CASC9/microRNA(miR)-590–3p/sine oculis homeobox 1 (SIX1)/NF-κB axis promotes proliferation and migration in breast cancer

Long non-coding RNA (LncRNA) CASC9/microRNA(miR)-590–3p/sine oculis homeobox 1 (SIX1)/NF-κB axis promotes proliferation and migration in breast cancer

The genetic architecture of human amygdala volumes and their overlap with common brain disorders

Development and Evaluation of a Novel Cuproptosis‐Related lncRNA Signature for Gastric Cancer Prognosis

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