Long non-coding RNAs RP5-821D11.7, APCDD1L-AS1 and RP11-277P12.9 were associated with the prognosis of lung squamous cell carcinoma

Abstract

Lung squamous cell carcinoma (LUSC), a type of non-small cell lung carcinoma, has a poor therapeutic response, high relapse rate and poor prognosis. The present study was designed to reveal the key long non-coding RNAs (lncRNAs) associated with the prognosis of LUSC. The lncRNA expression profiles of LUSC and adjacent samples were downloaded from The Cancer Genome Atlas database. Based on the edgeR and DEseq packages, the differentially expressed lncRNAs (DELs) between LUSC and adjacent samples were obtained and the intersecting DELs were regarded as significant DELs. Subsequently, a prognostic risk model was established using Cox regression analysis and its classification effect was detected by survival analysis. Using survival analysis, the effect of the prognostic risk model was assessed in the validation set and other types of cancer. Finally, the co-expression genes of key lncRNAs were screened using the Multi-Experiment Matrix tool and the STRING database, and their functions were predicted via enrichment analysis using the Database for Annotation, Visualization and Integrated Discovery tool. A total of 2,041 significant DELs between LUSC and adjacent samples were screened. The prognostic risk model consisting of RP5-821D11.7, APCDD1L-AS1 and RP11-277P12.9 was established, which had a good classification effect. Cox multivariate regression analysis demonstrated that risk score may serve as an independent prognostic factor. Furthermore, certain co-expression genes of RP5-821D11.7 (including proliferating cell nuclear antigen), APCDD1L-AS1 (including semaphorin 5A, semaphorin 6D, ADAMTS like 1, ADAM metallopeptidase with thrombospondin type 1 motif 6, slit guidance ligand 3, and tenascin C) and RP11-277P12.9 (including Wnt family member 2B) were identified. Additionally, ‘positive regulation of cell migration’ and ‘proteinaceous extracellular matrix’ were enriched. In conclusion, the expression levels of the lncRNAs RP5-821D11.7, APCDD1L-AS1 and RP11-277P12.9 may affect the prognosis of LUSC.

Comprehensive landscape of epigenetic-dysregulated lncRNAs reveals a profound role of enhancers in carcinogenesis in BC subtypes

“…APCCD1L-AS1 is reported to significantly distinguish the high-expression group from the low-expression group in lung squamous cell carcinoma. 28 Each of these modules was able to significantly distinguish patients in high-risk groups from those in low-risk groups in terms of overall survival in the luminal subtype ( Figure 5 D). We obtained an RNA-seq dataset of 42 ER-positive (ER + ) breast cancer primary tumors and 30 adjacent breast tissues (GEO: GSE58135 ) to further validate the pattern of lncRNA expression in independent clinical samples.…”

Molecular characterization of papillary thyroid carcinoma: a potential three-lncRNA prognostic signature

“…Moreover, increasing evidence indicates that lncRNAs play a potential role as novel biomarkers for prognostic prediction in various cancers. 10 , 11 It was found that a number of lncRNAs were correlated with the prognosis of PTC. Liao et al 12 reported that BANCR could inhibit tumorigenesis in PTC by performing quantitative real-time PCR (qRT-PCR), cell function and tumor xenograft in nude mice, suggesting that BANCR may serve as a novel prognostic marker.…”

LINC00511 promotes lung squamous cell carcinoma proliferation and migration via inhibiting miR-150-5p and activating TADA1

“…Some lncRNA such as LncRNAs RP5-821D11.7, HULC have been implicated to contribute to the unfavourable prognosis of LUSC (21,22). Recently, LINC00511 has been identified to be the critical factors for carcinogenesis and metastasis in tumors, including glioma, bladder cancer, breast cancer, non-small cell lung cancer (19,(23)(24)(25).…”