Long Noncoding RNA AFAP1-AS1 Promoted Tumor Growth and Invasion in Cholangiocarcinoma

Xu, Lei; Zhou, Chen; Li, Renfeng; Deng, Yong; Zhao, Longshuan; Zhai, Wenlong

doi:10.1159/000477319

Cited by 65 publications

(53 citation statements)

References 27 publications

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“…Studies have shown that aberrant lncRNA expression influences cancer progression and can predict patient outcome [12, 24]. In the present study, we examined the expression of SNHG12 in cervical cancer tissues, compared to the corresponding normal tissues, and we found that SNHG12 was significantly upregulated in cervical cancer tissue and that high SNHG12 was positively associated with FIGO stage, lymph node metastasis and a poor prognosis.…”

Section: Discussionmentioning

confidence: 69%

Upregulation of Long Non-Coding RNA Small Nucleolar RNA Host Gene 12 Contributes to Cell Growth and Invasion in Cervical Cancer by Acting as a Sponge for MiR-424-5p

Dong

Wang

et al. 2018

Cell Physiol Biochem

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Background/Aims: Cervical cancer, which is one of the most aggressive cancers affecting females, has high rates of recurrence and mortality. Small nucleolar RNA host gene 12 (SNHG12) is known to promote the progression of several cancers; however, its exact effects and molecular mechanisms in cervical cancer remain unknown. Methods: Real-time quantitative PCR was used to determine the expression level of SNHG12 in cervical cancer tissues and cell lines. Loss-of-function assays were performed to examine the effect of SNHG12 on the proliferation, apoptosis, migration and invasion of cervical cancer cells in vitro and tumor growth in vivo. Luciferase experiments were employed to explore the interactions between SNHG12 and miR-424-5p. Results: SNHG12 was found to be abnormally elevated in human cervical cancer tissues compared with paired adjacent normal tissues. Moreover, high SNHG12 expression in tumor tissues was significantly correlated with vascular involvement, lymph node metastasis, advanced FIGO stage and poor prognosis. Furthermore, the knockdown of SNHG12 was found to inhibit proliferation, migration and invasion of cervical cancer cells in vitro, and silencing SNHG12 was shown to suppress tumor growth in a nude mouse model. Mechanistic studies showed that SNHG12 functioned as an endogenous sponge for miR-424-5p, thereby downregulating the expression of miR-424-5p in cervical cancer. Furthermore, the inhibition of miR-424-5p in SNHG12-depleted cells partially reversed the effects on cervical cancer cell apoptosis, adhesion and invasion. Conclusion: In summary, our findings suggest that the tumor-promoting role of SNHG12 is to function as a molecular sponge, which negatively regulates miR-424-5p. These findings may provide a potent therapeutic target for cervical cancer.

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Section: Discussionmentioning

confidence: 69%

Upregulation of Long Non-Coding RNA Small Nucleolar RNA Host Gene 12 Contributes to Cell Growth and Invasion in Cervical Cancer by Acting as a Sponge for MiR-424-5p

Dong

Wang

et al. 2018

Cell Physiol Biochem

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“…Nowadays, numerous lncRNAs have been reported to play important role in carcinogenesis at both transcriptional and post-transcriptional levels, but their specific mechanisms of action still remain elusive [5, 18, 19, 20]. H19 has been identified as one of the lncRNAs involved in tumorigenesis, however, its role in tumor proliferation and apoptosis has been a subject of controversy for a long time.…”

Section: Discussionmentioning

confidence: 99%

Long Noncoding RNA H19/miR-675 Axis Promotes Gastric Cancer via FADD/Caspase 8/Caspase 3 Signaling Pathway

Yan¹,

Zhang²,

Qiang³

et al. 2017

Cell Physiol Biochem

109

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Background: Long noncoding RNA (lncRNA) H19 is emerging as a vital regulatory molecule in the progression of different types of cancer and miR-675 is reported to be embedded in H19's first exon. However, their function and specific mechanisms of action have not been fully elucidated. The aim of this study was to identify a novel lncRNA-microRNA-mRNA functional network in gastric cancer. Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) was used to assess the relative expression of H19 and miR-675 in normal (GES-1) and gastric cancer cell lines (SGC-7901, SGC-7901/DDP) as well as in tumor tissues. Gain and loss of function approaches were carried out to investigate the potential roles of H19/miR-675 in cell proliferation and apoptosis. Moreover, Fas associated via death domain (FADD) was validated to be the target of miR-675 via luciferase reporter assay. Western blotting was used to evaluate the protein expression of related signaling pathway. Results: In our study H19 and miR-675 were increased in gastric cancer cell lines and tissues. Overexpression of H19 and miR-675 promoted cell proliferation and inhibited cell apoptosis, whereas knockdown of H19 and miR-675 inhibited these effects. By further examining the underlying mechanism, we showed that H19/miR-675 axis inhibited expression of FADD. FADD downregulation subsequently inhibited the caspase cleavage cascades including caspase 8 and caspase 3. Conclusion: Taken together, our results point to a novel regulatory pathway H19/miR-675/ FADD/caspase 8/caspase 3 in gastric cancer which may be potential target for cancer therapy.

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“…30,35,[40][41][42][43][44][45][46][47][48][49][50][51][52][53][54][55][56][57][58] In this review, the related clinicopathological characteristics and molecular functions of this lncRNA in cancers are presented in Tables 1 and 2.…”

Section: Identification Of Afap1-as1mentioning

confidence: 99%

AFAP1‐AS1: A novel oncogenic long non‐coding RNA in human cancers

Zhang

Xiao

et al. 2017

Cell Proliferation

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Long Noncoding RNA AFAP1-AS1 Promoted Tumor Growth and Invasion in Cholangiocarcinoma

Cited by 65 publications

References 27 publications

Upregulation of Long Non-Coding RNA Small Nucleolar RNA Host Gene 12 Contributes to Cell Growth and Invasion in Cervical Cancer by Acting as a Sponge for MiR-424-5p

Upregulation of Long Non-Coding RNA Small Nucleolar RNA Host Gene 12 Contributes to Cell Growth and Invasion in Cervical Cancer by Acting as a Sponge for MiR-424-5p

Long Noncoding RNA H19/miR-675 Axis Promotes Gastric Cancer via FADD/Caspase 8/Caspase 3 Signaling Pathway

AFAP1‐AS1: A novel oncogenic long non‐coding RNA in human cancers

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