Long Noncoding RNA CCAT1 Functions as a Competing Endogenous RNA to Upregulate ITGA9 by Sponging MiR-296-3p in Melanoma [Retraction]

Fan, Jinghua; Kang, Xiaoxiao; Zhao, Limin; Zheng, Yan; Yang, Jun; Li, Di

doi:10.2147/cmar.s352908

Cited by 3 publications

(6 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…RNA Binding Protein Immunoprecipitation (RIP). This assay was performed as previously described [18]. Using Magna RIP RNA-Binding Protein Immunoprecipitation Kit, RIP assay was carried out.…”

Section: Fluorescencementioning

confidence: 99%

See 1 more Smart Citation

[Retracted] circZC3HAV1 Regulates TBC1D9 to Affect the Biological Behavior of Colorectal Cancer Cells

Zhang

Xue

Gao

et al. 2022

BioMed Research International

View full text Add to dashboard Cite

Background Colorectal cancer (CRC) is one of the most frequently diagnosed cancers all over the world, which accounts for a large proportion of cancer-associated deaths. The regulatory function of circular RNAs (circRNAs) has been affirmed in diverse cancers. circ_0082628, named circRNA zinc finger CCCH-type containing antiviral 1 (circZC3HAV1), has been discovered to be significantly downregulated in CRC tissues. Nevertheless, the function and mechanism of circZC3HAV1 in CRC remain unclear. Purpose We targeted at studying the specific role and mechanism of circZC3HAV1 in CRC cells. Methods The expression of the genes was detected by quantitative real-time polymerase chain reaction (qPCR). The binding relationship among different genes was verified by mechanism assays. Functional assays were carried out to reveal the role of different RNAs in CRC cell malignant behaviors. Results circZC3HAV1 was significantly downregulated in CRC cells. circZC3HAV1 overexpression hampered CRC cell migratory and invasive abilities. As for the mechanism, circZC3HAV1 competitively bound with microRNA-146b-3p (miR-146b-3p) to enhance the expression of TBC1 domain family member 9 (TBC1D9). Rescue assays demonstrated circZC3HAV1 sponged miR-146b-3p and upregulated TBC1D9 to restrict migration and invasion of CRC cells. Conclusion circZC3HAV1 could upregulate TBC1D9 via absorbing miR-146b-3p, consequently inhibiting migratory and invasive capabilities of CRC cells.

show abstract

Section: Fluorescencementioning

confidence: 99%

“…Luciferase Reporter Assay. The assay was carried out as previously described [18]. Full-length of TBC1D9 3′ untranslated regions (3′UTR) covering wild-type or mutant miR-146b-3p binding sites was inserted into pmirGLO luciferase vectors to construct pmirGLO+TBC1D9 3′UTR-WT/ MUT.…”

Section: Fluorescencementioning

confidence: 99%

[Retracted] circZC3HAV1 Regulates TBC1D9 to Affect the Biological Behavior of Colorectal Cancer Cells

Zhang

Xue

Gao

et al. 2022

BioMed Research International

View full text Add to dashboard Cite

show abstract

“…EZ-Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore, Billerica, Massachusetts, USA) was utilized for the RNA-binding protein immunoprecipitation (RIP) experiment [31]. Cell lysates were incubated with magnetic beads, anti-AGO2 (Millipore), and anti-IgG (Millipore).…”

Section: Methodsmentioning

confidence: 99%

“…This assay was carried out as previously described [31]. LINC01063-WT/MUT and SOX12-WT/MUT luciferase vectors containing indicated miR-5194 sequences were generated.…”

Section: Luciferase Reporter Assaymentioning

confidence: 99%

LINC01063 functions as an oncogene in melanoma through regulation of miR-5194-mediated SOX12 expression

Nie

et al. 2022

Melanoma Research

View full text Add to dashboard Cite

Melanoma is one of the most aggressive skin cancers and a major cause of cancer-linked deaths worldwide. As the morbidity and mortality of melanoma are increasing, it is necessary to elucidate the potential mechanism influencing melanoma progression. Tumor tissues and adjacent normal tissues (5 cm away from tumors) from 22 melanoma patients at the I–II stage and 39 patients at the III–VI stage were acquired. The expression of LINC01063 in melanoma was estimated by quantitative PCR. Functional assays were employed to investigate the function of LINC01063 in melanoma. Mechanism assays were adopted to explore the mechanism of LINC01063. LINC01063 knockdown impeded melanoma cell proliferation, migration, invasion, and epithelial–mesenchymal transition as well as melanoma tumor growth. Mechanistically, LINC01063 acted as an miR-5194 sponge to upregulate SOX12 expression. Finally, LINC01063 was tested to facilitate the malignant behaviors of melanoma cells via targeting miR-5194/SOX12. LINC01063 was significantly upregulated in melanoma. Specifically, LINC01063 displayed a higher level in patients at an advanced stage or with metastasis than those at an early stage or without metastasis. Our study revealed the oncogenic effects of LINC01063 on melanoma cell/tumor growth and its molecular mechanism involving miR-5194/SOX12, which might support LINC01063 to be the potential prognostic or therapeutic biomarker against melanoma.

show abstract

“…21 Several studies have indicated that miR-296-3p is involved in many diseases, including osteoarthritis, polycystic ovary syndrome, melanoma and diabetic nephropathy. [22][23][24][25] miR-296-3p and its downstream networks are involved in the resistance of mammalian pancreatic α-cells to cytokine-induced apoptosis. 26 miR-296-3p has also been shown to be involved in the accumulation of extracellular matrix in mesangial cells in diabetic nephropathy.…”

Section: Introductionmentioning

confidence: 99%

Role of lncRNA‐MEG8/miR‐296‐3p axis in gestational diabetes mellitus

et al. 2022

View full text Add to dashboard Cite

Aim Gestational diabetes mellitus (GDM) is the most common complication in pregnancy. This study aimed to investigate the potential mechanism and effects of long‐noncoding RNA maternally expressed 8 (lncRNA‐MEG8) in GDM. Methods Targeted interactions involving lncRNA‐MEG8 and miR‐296‐3p were initially predicted using starBase software and then confirmed using dual‐luciferase reporter gene analysis. The expression levels of lncRNA‐MEG8 and miR‐296‐3p in peripheral blood samples from patients with GDM were measured using reverse transcription‐quantitative polymerase chain reaction. Enzyme‐linked immunosorbent assay was used to evaluate the overall levels of insulin and insulin secretion. Additionally, MTT and flow cytometric methods were used to detect cell viability and apoptosis. Cell apoptosis‐associated proteins were determined by western blotting. Results Our results indicated that lncRNA‐MEG8 is a potential target of miR‐296‐3p. lncRNA‐MEG8 level was higher, whereas that of miR‐296‐3p was lower in patients with GDM than in healthy individuals. LncRNA‐MEG8‐siRNA promoted insulin content and secretion. Furthermore, MEG8‐siRNA increased cell viability and decreased apoptosis. However, these changes were reversed by an miR‐296‐3p inhibitor. Moreover, a miR‐296‐3p mimic had the same effect on INS‐1 cells as MEG8‐siRNA, as evidenced by enhanced insulin secretion, cell viability, and reduced apoptosis. Conclusion LncRNA‐MEG8‐siRNA promotes pancreatic β‐cell function by upregulating miR‐296‐3p.

show abstract

Long Noncoding RNA CCAT1 Functions as a Competing Endogenous RNA to Upregulate ITGA9 by Sponging MiR-296-3p in Melanoma [Retraction]

Cited by 3 publications

References 0 publications

[Retracted] circZC3HAV1 Regulates TBC1D9 to Affect the Biological Behavior of Colorectal Cancer Cells

[Retracted] circZC3HAV1 Regulates TBC1D9 to Affect the Biological Behavior of Colorectal Cancer Cells

LINC01063 functions as an oncogene in melanoma through regulation of miR-5194-mediated SOX12 expression

Role of lncRNA‐MEG8/miR‐296‐3p axis in gestational diabetes mellitus

Contact Info

Product

Resources

About