Long Noncoding RNA Expression Profiles of Lung Adenocarcinoma Ascertained by Microarray Analysis

Abstract

BackgroundLong noncoding RNAs (lncRNAs) have been shown to be involved in the development and progression of lung cancer. However, the roles of lncRNAs in lung cancer are not well understood.Methodology/Principal FindingsWe used a high-throughput microarray to compare the lncRNA and messenger RNA (mRNA) expression profiles in lung adenocarcinoma and normal tissue (NT) samples. Several candidate adenocarcinoma-associated lncRNAs were verified by real-time quantitative reverse transcription polymerase chain reaction (PCR) analysis. Using abundant and varied probes, we were able to assess 30,586 lncRNAs and 26,109 mRNAs in our microarray. We found that 2,420 lncRNAs and 1,109 mRNAs were differentially expressed (≥2-fold change) in lung adenocarcinoma samples and NT, indicating that many lncRNAs were significantly upregulated or downregulated in lung adenocarcinoma. We also found, via quantitative PCR, that 19 lncRNAs were aberrantly expressed in lung adenocarcinoma compared with matched histologically normal lung tissues. Among these, LOC100132354 and RPLP0P2 were the most aberrantly expressed lncRNAs, as estimated by quantitative PCR in 100 pairs of lung adenocarcinoma and NT samples.Conclusions/SignificanceOur study ascertained the expression patterns of lncRNAs in lung adenocarcinoma by microarray. The results revealed that many lncRNAs were differentially expressed in lung adenocarcinoma tissues and NT, suggesting that they may play a key role in tumor development.

Long intergenic non-protein coding RNA 00858 functions as a competing endogenous RNA for miR-422a to facilitate the cell growth in non-small cell lung cancer

“…Recently, Xu and his colleagues has reported that LINC00858 is highly expressed in lung adenocarcinoma (5.23-fold than normal lung tissues) [12], while until now, the relevance between LINC00858 expression and NSCLC tumorigenesis has not been elaborated yet. Hence, the roles and potential biological mechanisms of LINC00858 on NSCLC still remain to be expounded.…”

Clinical significance and effect of AEG-1 on the proliferation, invasion, and migration of NSCLC: a study based on immunohistochemistry, TCGA, bioinformatics, in vitro and in vivo verification

“…Lung cancer has become the most common cancer, and the incidence rate of lung cancer is gradually growing [1–4]. About 80%–85% of the newly diagnosed lung cancers are non-small cell lung cancer (NSCLC) [5, 6].…”

Long Non-Coding RNAs and Their Roles in Non-Small-Cell Lung Cancer

“…For instance, using high-throughput microarrays, Xu et al identified 2420 lncRNAs that were differentially expressed (fold change ⩾2) between LAD and normal tissue (NT) samples. Of these 2420 lncRNAs, the expression of 1213 lncRNAs was upregulated, whereas the expression of the remaining 1207 lncRNAs was downregulated [48]. As another example, Yang et al identified 47 lncRNAs (14 upregulated and 33 downregulated lncRNAs) from gene expression data of five NSCLC cohorts that were deposited in the Gene Expression Omnibus (GEO) database [49].…”