Long Noncoding RNA LINC00467 Promotes Glioma Progression through Inhibiting P53 Expression via Binding to DNMT1

Abstract

This study aimed to investigate whether long noncoding RNA (lncRNA) LINC00467 could regulate proliferative and invasive abilities of glioma cells via p53 and DNA methyltransferase 1 (DNMT1), so as to participate in the occurrence and progression of glioma. Methods: LINC00467 expression in glioma was analyzed by GEPIA database and LINC00467 expression in glioma cell lines was detected by qRT-PCR. The regulatory effects of LINC00467 and p53 on proliferative, invasive capacities and cell cycle were conducted by CCK-8 and EdU assays, transwell assay and flow cytometry, respectively. The binding conditions between LINC00467, DNMT1 and p53 were determined by RNA immunoprecipitation (RIP) and Chromatin immunoprecipitation (ChIP) assays. Western blot was conducted to determine whether LINC00467 could regulate p53 in glioma cells. Finally, rescue experiments were carried out to evaluate whether LINC00467 regulates proliferative and invasive abilities of glioma cells through p53. Results: The expression of LINC00467 was significantly up-regulated in tumor samples than that in normal samples, which was not correlated with patient survival time. Besides, expression of LINC00467 was higher in glioma cells than that of negative control cells. Upregulation of LINC00467 promoted proliferative and invasive abilities, and accelerated cell cycle in G0/G1 phase of U87 and LN229 cells. The results of RIP and ChIP assays demonstrated that LINC00467 could bind to DNMT1 and inhibit p53 expression. Overexpression of p53 partially reversed the enhancement of LINC00467 on proliferative and invasive abilities of glioma cells. Conclusion: These results indicated that high expression of LINC00467 could promote proliferative and invasive abilities of glioma cells through targeting inhibition of p53 expression by binding to DNMT1.

<p>Long Noncoding RNA LINC00173 Promotes NUTF2 Expression Through Sponging miR-765 and Facilitates Tumorigenesis in Glioma</p>

“…15 LncRNA LINC00467 upregulation promotes glioma development through repressing P53 level. 16 Previous study showed that LINC00173 downregulation promotes non-small cell lung cancer cell growth and survival. 17 However, another study showed that Linc00173 enhances chemoresistance and facilitates tumor progression in small cell lung cancer.…”

Expression and prognostic characteristics of m5C regulators in low‐grade glioma

“…This study conducted enrichment analysis of m 5 C regulator‐related factors, and the results showed that these factors were enriched in items such as DNA replication, chromosome segregation, nuclear division, cell cycle and P53 signalling pathway. It is reported that up‐regulated LINC00467 can bind to DNMT1 and inhibit p53 level and promote glioma cells proliferation and invasion 22 . Furthermore, lncRNA SNHG7 regulates p53 expression and arrests cell cycle in the G0/G1 phase of osteosarcoma by binding with DNMT1 23 .…”

LncRNA LINC00467 acted as an oncogene in esophageal squamous cell carcinoma by accelerating cell proliferation and preventing cell apoptosis via the miR‐485‐5p/DPAGT1 axis

“…The aberrant expression of LINC00467 has been identified in diverse tumors like hepatocellular carcinoma, glioma, and neuroblastoma 16,34,35 . Especially, upregulated LINC00467 exhibited oncogenic roles to promote cell proliferation or prevented cell apoptosis in lung adenocarcinoma 36 .…”