Long Noncoding RNASBF2-AS1 Promotes Gastric Cancer Progression via Regulating miR-545/EMS1 Axis

Abstract

Objective. Long noncoding RNA (LncRNA) SBF2-AS1 was reportedly to function as an oncogene in several types of cancers, such as hepatocellular carcinoma, nonsmall cell lung cancer, glioma, and colorectal cancer. However, the biological roles and regulatory mechanisms of SBF2-AS1 in gastric cancer (GC) are unknown. Methods. The expression of SBF2-AS1 and miR-545 were examined in GC tissues and cell lines via real-time quantitative PCR. The relationship of SBF2-AS1 with miR-545 was verified via dual-luciferase reporter gene assay and RNA immunoprecipitation. The influences of SBF2-AS1 on cell proliferation, migration, and invasion were determined using cell counting Kit-8 (CCK-8), wound healing, and transwell invasion assays, respectively. Results. LncRNA SBF2-AS1 expression was upregulated in GC tissues, especially in advanced clinical stage cases. Moreover, increased SBF2-AS1 indicated a poor survival rate. Functionally, the downregulation of SBF2-AS1 by siRNA in GC cells suppressed the proliferation, migration, and invasion. In terms of mechanism, SBF2-AS1 can directly bind to miR-545 and regulate its expression. Moreover, SBF2-AS1 knockdown significantly decreased the expression of EMS1, which was the direct target of miR-545. Importantly, inhibition of miR-545 or overexpression of EMS1 partially reversed SBF2-AS1-depletion-caused suppression on proliferation, migration, and invasion. Conclusion. These findings elucidated a crucial role of SBF2-AS1 as a miR-545 sponge in GC cells, suggesting that SBF2-AS1 might be a potential target for GC.

RETRACTED ARTICLE: Long non-coding RNA PTPRG-AS1 promotes cell tumorigenicity in epithelial ovarian cancer by decoying microRNA-545-3p and consequently enhancing HDAC4 expression

“…Differentially expressed miR-545-3p has been observed in diverse human malignancies [ 46 – 48 ]. In EOC, miR-545-3p is downregulated in EOC tissues and cell lines [ 34 ].…”

MicroRNA-545 suppresses progression of ovarian cancer through mediating PLK1 expression by a direct binding and an indirect regulation involving KDM4B-mediated demethylation

“…In a molecular perspective, miR-545 overexpression led to an increase to the Bax/Bcl-2 ratio, as well as an increase in E-cadherin expression while a decline in the expression of N-cadherin and Vimentin. The tumor-suppressing role of miR-545 has been well demonstrated, such as suppressing cell proliferation and metastasis in cervical cancer [7], gastric cancer [25], colon adenocarcinoma [26] and so forth, and enhancing the radiosensitivity of Lewis xenograft tumor [27]. These reports suggested miR-545 as a promising and specific miRNA for cancer management.…”

The molecular mechanisms of the long noncoding RNA SBF2-AS1 in regulating the proliferation of oesophageal squamous cell carcinoma

“…In this study, we found that SBF2-AS1 was highly expressed in a variety of tumours and then analysed the TCGA database, which revealed that the expression level of SBF2-AS1 was significantly higher in oesophageal squamous cell carcinoma tissues than in normal tissues. Previous studies have shown that SBF2-AS1 can promote the development of gastric cancer through sponging miR-545 31 . Next, we wanted to determine whether SBF2-AS1 sponges other miRNAs to regulate the development of oesophageal squamous cell carcinoma.…”