Long-Term Comparison of Rib and Ear Cartilage Grafts in Autologous and Allogenic Fascia Lata: An Experimental Study in a White Rabbit Model

Jurk, Viktor; Kampmann, H; Iblher, Niklas; Bannasch, Holger; Gubisch, Wolfgang

doi:10.1097/prs.0000000000002133

Cited by 10 publications

(11 citation statements)

References 33 publications

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“…However, in comparison with other source of chondrocytes, CCs have stronger tendency of hypertrophy and calcification [ [7] , [8] , [9] , [10] ]. Hypertrophy is the terminal differentiation stage of growth plate chondrocyte, which is a necessary developmental stage in endochondral ossification during normal bone formation and growth [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Anti-hypertrophic effect of synovium-derived stromal cells on costal chondrocytes promotes cartilage repairs

Zheng

Pang

et al. 2022

Journal of Orthopaedic Translation

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Background Costal chondrocytes (CCs), as a promising donor cell source for cell-based therapy for cartilage repair, have strong tendency of hypertrophy and calcification, which limited CCs from further application in cartilage regenerative medicine. Synovium-derived stromal cells (SDSCs), have shown their beneficial effect for chondrocytes to maintain phenotype. This study aims to investigate whether SDSCs could help CCs to maintain chondrogenic phenotype and suppress hypertrophic differentiation in cartilage repairs. Methods CCs were directly cocultured with SDSCs in pellet or indirectly cocultured using a conditioned medium in vitro for 3 weeks. Cartilage matrix formation and hypertrophic differentiation of CCs were analyzed by RT-PCR, biochemical assays, and histological staining. Cocultured pellets were implanted into the osteochondral defects made on the femoral groove of the rats. Then, macroscopic and histological evaluations were performed. Results Pellets formed by CCs alone and CCs cocultured with SDSCs reveal equal cartilage matrix deposition. However, the gene expression of type X collagen was significantly downregulated in cocultured pellets. Immunohistochemistry analysis revealed suppressed expression of type X collagen in cocultured pellets, indicating SDSCs may suppress hypertrophic differentiation of chondrocytes. Further in indirect coculture experiment, SDSCs suppressed type X collagen expression as well and promoted the proliferation of CCs, indicating SDSCs may influence CCs by paracrine mechanism. The pellets implanted in the osteochondral defects showed good restoration effects, whereas the grafts constructed with CCs and SDSCs showed lower type X expression levels. Conclusion These results suggest that SDSCs may maintain the phenotype of CCs and prevent the hypertrophic differentiation of CCs in cartilage repair. The Translational Potential of this Article : CCs is a promising donor cell source for cell-based therapy for cartilage repair. Based on our study, cocultured with SDSCs weakened the tendency of hypertrophy and calcification of CCs, which provide a potential usage of SDSCs in CCs-based cartilage repair therapy to suppress newly formed cartilage calcification and improve clinical outcomes.

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Section: Introductionmentioning

confidence: 99%

Anti-hypertrophic effect of synovium-derived stromal cells on costal chondrocytes promotes cartilage repairs

Zheng

Pang

et al. 2022

Journal of Orthopaedic Translation

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“…34 Wrapping diced cartilage in an Allo-Derm sleeve or in Tutoplast-processed fascia lata instead of temporalis fascia has been said to be a viable alternative. 21,22 In rats, both solid and diced cartilages wrapped in amniotic membrane had a higher success rate than bare cartilage grafts. 40 To overcome adding a second surgical site to the procedure by harvesting temporalis fascia, diced cartilage has been successfully wrapped in rectus abdominis fascia and pectoralis major fascia being in the costal cartilage donor area.…”

Section: Fascia and Alternative Wrappingsmentioning

confidence: 99%

“…18 Homologous lyophilized bone and fascia lata have also been used with recently renewed interest in the use of processed fascia lata. [19][20][21][22] To overcome the incongruent shape of solid cartilage grafts, septal cartilage in particular, weakening of grafts by crushing has been widely used in the past. The degree of crushing is known to have a strong impact on resorption rates with intact or mildly crushed cartilage having a better survival in retrospective clinical and animal studies.…”

Section: Autologous and Homologous Tissues For Dorsal Augmentationmentioning

confidence: 99%

“…36,37 Today, for many surgeons, the DCF graft is considered to be the gold standard for dorsal augmentation as it combines versatility with the use of autologous tissues, preferred by many patients and surgeons. 4,21,38 One obvious drawback of the diced cartilage in fascia technique is the need to harvest a 5-Â 5-cm sheet of deep temporal fascia for a sleeve 3 and sewing a recipient pocket adding time to the procedure. Numerous attempts have been made to simplify the procedure.…”

Section: Fascia and Alternative Wrappingsmentioning

confidence: 99%

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Dorsal Augmentation-Diced Cartilage Techniques: The Diced Cartilage Glue Graft

Tasman

2017

Facial plast Surg

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The quest for the ideal method for augmenting the nasal dorsum continues to be a matter of debate, with both most surgeons and patients preferring autologous tissue. This article reviews the current use of diced cartilage for nasal augmentation, emphasizing the diced cartilage in fibrin glue (DCG) graft. It offers the first collation of unfavorable outcomes and complications of the DCG graft seen in a series of 108 patients treated at the author's institution. The DCG graft continues to be a versatile graft that is stable over time and combines unique features. It has proved to be particularly well suited for segmental augmentations of the dorsum. Resorption of the graft has been rare and infrequent unfavorable outcomes have all been amenable to successful minor surgical revisions.

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“…15,16 To avoid a second operative site and related potential complications, diced cartilage has also been successfully used with processed fascia lata for augmentation rhinoplasty. 17,18 However, Lan 19 and Yang 17 observed resorption and replacement of inserted fascia lata by the host's own fibrous tissue leading to scar contraction of the overlying skin and soft-tissue envelope. The senior author observed a significant shortening of the nose in 1.2% of patients who have been treated with processed fascia lata (personal communication with Yang YJ).…”

Section: (D) Wrapped Diced Cartilagementioning

confidence: 99%

Cartilage Scales Embedded in Fibrin Gel

Kovačević¹,

Riedel²,

Wurm

et al. 2017

Facial plast Surg

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Multiple techniques have been described for dorsal nasal augmentation in rhinoplasty. In this article, we review common surgical techniques for raising the dorsum or eliminating dorsal irregularities, by highlighting inherent advantages and disadvantages of each method. Within the past few years, the use of diced cartilage grafts has become the workhorse in this field of interest. To overcome drawbacks of methods based on diced cartilage, we present a new concept for autologous augmentation, using regenerative medicine protocols. A mix of cartilage scales with cartilage pâté was embedded in platelet-rich fibrin (PRF). Since December 2015, a total of 48 patients were treated with this technique. Based on our preliminary results, cartilage scales in PRF appear to be a promising and reliable alternative to existing procedures for dorsal nasal augmentation.

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Long-Term Comparison of Rib and Ear Cartilage Grafts in Autologous and Allogenic Fascia Lata: An Experimental Study in a White Rabbit Model

Abstract: This study shows the equivalency of diced cartilage in fascia grafts and isolated fascial grafts using allogenic fascia lata compared to autologous fascia. The type of cartilage used accounts for different long-term characteristics of diced cartilage in fascia grafts.

Cited by 10 publications

References 33 publications

Anti-hypertrophic effect of synovium-derived stromal cells on costal chondrocytes promotes cartilage repairs

Anti-hypertrophic effect of synovium-derived stromal cells on costal chondrocytes promotes cartilage repairs

Dorsal Augmentation-Diced Cartilage Techniques: The Diced Cartilage Glue Graft

Cartilage Scales Embedded in Fibrin Gel

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