Long-term culture of murine erythropoietic progenitor cells in the absence of an adherent layer

Wendling, Françoise; Shreeve, Mona M.; McLeod, Donald W.; Axelrad, Arthur A.

doi:10.1038/305625a0

Cited by 13 publications

(15 citation statements)

References 17 publications

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“…In contrast, the size of pure mast clones was increased and the mixed clones had a larger mast progeny. Like mast progenitors, mast cell lines Wendling et al, 1983) were resistant to CT (unpublished data). In spite of the fact that CT binding raised c-AMP levels, we failed to find any strong evidence of a enhanced growth rate of factor-dependent mast cell lines.…”

Section: Discussionmentioning

confidence: 92%

“…This regulator also ensured the growth of various factordependent cell lines such as basophil-mast and/or P cell (Nagao et al, 1981;Tertian et al, 1981;Hastorpe, 1980;Schrader, 1981), granulocytes (Dexter et al, 1980), mast/ erythroid progenitors (Wendling et al, 1983) and pluripotent progenitor lines (Greenberger et al, 1983). These new findings and others (Kitamura et al, 1981;Sonoda et al, 1982Sonoda et al, , 1983 contributed to demonstrating that basophil-mast cell progenitors are related to hemopoietic lineages and pointed chiefly to the multipotent hemopoietic stem cell as the primary basophil-mast ancestor.…”

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confidence: 96%

“…The obtention of mast cell lines or mast progenitor cell lines (Wendling et al, 1983), from bone marrow or bone marrow cultures became an easy 0 1986 ALAN R. LISS. INC. task; this contrasted with the reported paucity of colonies containing mast cells obtained in direct clonal assays (Nakahata et al, 1982;Suda et al, 1985).…”

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confidence: 99%

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Murine basophil—mast differentiation: Toward optimal conditions for selective growth and maturation of basophil—mast or allied cells

Lanotte

Arock

Lacaze

et al. 1986

Journal Cellular Physiology

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Recent investigations revealed that basophil-mast cells were related to the hemopoietic system. Strikingly, murine bone marrow showed a singular paucity in cells with basophil-mast features; moreover in clonogenic assays (methylcellulose, agarose) bone marrow was found to be manifestly poor in basophil-mast progenitor cells. Our work brought to light several new facts concerning the culture and differentiation of this cell type: 1 degree pure and mixed mast clones can be derived in large numbers from bone marrow, provided progenitors are cultured in collagen matrix. Up to 1,382 hemopoietic clones were analysed in situ after staining: 30% contained mast cells (34 per 10(5) cells), thus the basophil-mast lineage was one of the most frequent. We concluded that other cloning media were noticeably nonoptimal for the growth and/or maturation of mast cells. We suggested that collagen and the molecular edifices derived from it, both found in variable amounts in the natural mast environments, should play essential roles in mast phenotype expression. 2 Degrees cholera toxin (CT) selectively eradicated nonmast progenies: mast progenitors and mast progenies were resistant. In this way, pure and rapidly expanding mast cell clones were obtained at a frequency never reported before. CT possibly acts both directly, as a stimulator of mast cell proliferation, or indirectly on marrow subpopulations which repress basophil-mast cell growth and maturation. In vitro culture conditions, specifically designed for basophil-mast lineage, should prove of interest in the search for an unifying hypothesis concerning the multiple forms of mast cells found in various tissues.

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Section: Discussionmentioning

confidence: 92%

mentioning

confidence: 96%

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Murine basophil—mast differentiation: Toward optimal conditions for selective growth and maturation of basophil—mast or allied cells

Lanotte

Arock

Lacaze

et al. 1986

Journal Cellular Physiology

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“…Evidence for restricted progenitor cell types for erythroid and other lineages has been reported (41). The lag period for reconstitution (Fig.…”

Section: Discussionmentioning

confidence: 99%

Long-term cultured B lymphocytes and their precursors reconstitute the B-lymphocyte lineage in vivo.

Kurland¹,

Ziegler²,

Witte³

1984

Proc. Natl. Acad. Sci. U.S.A.

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Long-term in vitro cultured B lymphocytes and their precursors were tested for their ability to reconstitute a functional B-cell lineage in vivo. Continuous in vitro production of cells representing early stages of the B-lymphocyte lineage originates from a bone marrow cell type that can be distinguished from a multipotential hematopoietic stem cell. Progenitors of the long-term cultured B-lymphoid cells are recoverable in vitro after in vivo passage into lethally irradiated mice. Long-term cultured B-lymphoid cells and their progenitors reconstituted the B-lymphocyte lineage and restored normal humoral immunity in genetically defective mice. Cultured B-lymphoid cells did not cause lymphoid tumors in vivo in irradiated syngeneic mice. These findings indicate that multipotential hematopoietic stem cells are not directly required for the continued production of functionally competent B lymphocytes in vitro, and they suggest the existence of a B lymphoid progenitor cell with in vitro, and possibly in vivo, stem cell-like qualities.

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“…We have reported the continuous production of erythropoietic progenitor cells in long-term suspension culture from murine bone marrow cells established in the presence of pokeweed mitogen-stimulated spleen cell condictioned medium (PWSCM) and in the absence of a n adherent stromal cell layer (Wendling et al, 1983). We were unable to detect any progenitors of granulocyte, macrophage, megakaryocyte, eosinophil, or mixed colonies in these cell lines.…”

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confidence: 95%

A self‐renewing, bipotential erythroid/mast cell progenitor in continuous cultures of normal murine bone marrow

Wendling

Shreeve

McLeod

et al. 1985

Journal Cellular Physiology

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We have established permanent lines of nonadherent cells from fresh normal mouse bone marrow in media containing pokeweed mitogen-stimulated spleen cell conditioned medium (PWSCM). These lines continuously produced erythropoietic progenitor cells (detected by their ability to form erythroid bursts in semi-solid medium containing erythropoietin) together with cells having characteristics of the mast cell lineage (as demonstrated by metachromatic staining with toluidine blue, histamine content and membrane receptors for IgE). Sixteen such cell lines have been established in sixteen attempts. Cloning experiments were carried out to determine the nature of the progenitor cell(s) responsible for the permanence of these cultures. When cells were cultured in methylcellulose medium containing PWSCM, colonies were observed which reached macroscopic size after 4 weeks of incubation. Replating of individual primary colonies resulted in secondary colony formation, indicating the presence of progenitor cells with self-renewal potential. Forty-seven primary colonies were picked and their cells were suspended in liquid culture medium containing PWSCM. Of these, twenty-one could be expanded to establish permanently growing sublines. Sixteen of these sublines were found to be composed of both erythroid progenitors and mast cells. In five sublines only mast cells could be seen; none of the sublines appeared to be purely erythroid. Karyotypic analysis of mast cells and of erythroid cells of seven sublines derived from individual colonies which arose in cocultures of male and female cells revealed that the mast cells and erythroid cells were both of the same sex in each of the seven sublines; this demonstrates the single cell origin of each colony and of the two lineages derived from it. We conclude that these nonadherent, factor-dependent cell lines are maintained by self-renewal and differentiation of bipotential progenitor cells apparently restricted to the erythroid and mast cell lineages.

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Long-term culture of murine erythropoietic progenitor cells in the absence of an adherent layer

Cited by 13 publications

References 17 publications

Murine basophil—mast differentiation: Toward optimal conditions for selective growth and maturation of basophil—mast or allied cells

Murine basophil—mast differentiation: Toward optimal conditions for selective growth and maturation of basophil—mast or allied cells

Long-term cultured B lymphocytes and their precursors reconstitute the B-lymphocyte lineage in vivo.

A self‐renewing, bipotential erythroid/mast cell progenitor in continuous cultures of normal murine bone marrow

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