Long-term culture of SH-SY5Y neuroblastoma cells in the absence of neurotrophins: A novel model of neuronal ageing

Strother, Lisa; Miles, Gareth B.; Holiday, Alison; Cheng, Ying; Doherty, Gayle H.

doi:10.1016/j.jneumeth.2021.109301

Cited by 26 publications

(18 citation statements)

References 61 publications

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“…Potential explanations for the observed lipid changes and potential beneficial properties of vitamin B12 in respect to AD are given in the Supplementary Discussion as further background information. However, these explanations are based on literature and should not be overinterpreted without further experimental proof [ 78 , 79 , 80 , 81 , 82 , 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 , 92 , 93 , 94 , 95 , 96 , 97 , 98 , 99 , 100 , 101 , 102 , 103 , 104 , 105 , 106 , 107 , 108 , 109 , 110 , 111 , 112 , 113 , 114 , 115 , 116 , 117 , 118 , 119 , 120 ].…”

Section: Discussionmentioning

confidence: 99%

Vitamin B12 Attenuates Changes in Phospholipid Levels Related to Oxidative Stress in SH-SY5Y Cells

Theiss

Griebsch

Lauer

et al. 2022

Cells

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Oxidative stress is closely linked to Alzheimer’s disease (AD), and is detected peripherally as well as in AD-vulnerable brain regions. Oxidative stress results from an imbalance between the generation and degradation of reactive oxidative species (ROS), leading to the oxidation of proteins, nucleic acids, and lipids. Extensive lipid changes have been found in post mortem AD brain tissue; these changes include the levels of total phospholipids, sphingomyelin, and ceramide, as well as plasmalogens, which are highly susceptible to oxidation because of their vinyl ether bond at the sn-1 position of the glycerol-backbone. Several lines of evidence indicate that a deficiency in the neurotropic vitamin B12 is linked with AD. In the present study, treatment of the neuroblastoma cell line SH-SY5Y with vitamin B12 resulted in elevated levels of phosphatidylcholine, phosphatidylethanolamine, sphingomyelin, and plasmalogens. Vitamin B12 also protected plasmalogens from hydrogen peroxide (H2O2)-induced oxidative stress due to an elevated expression of the ROS-degrading enzymes superoxide-dismutase (SOD) and catalase (CAT). Furthermore, vitamin B12 elevates plasmalogen synthesis by increasing the expression of alkylglycerone phosphate synthase (AGPS) and choline phosphotransferase 1 (CHPT1) in SH-SY5Y cells exposed to H2O2-induced oxidative stress.

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Section: Discussionmentioning

confidence: 99%

Vitamin B12 Attenuates Changes in Phospholipid Levels Related to Oxidative Stress in SH-SY5Y Cells

Theiss

Griebsch

Lauer

et al. 2022

Cells

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“…We selected culture supernatants of hippocampal neurons cultured for 0, 7, and 14 days as the research object, which did not match the data source of biological analysis in a strict sense. We adopted this experimental design because we hoped to explore the changes in the intracellular environment specifically related to maturation. , Too short of a cell culture time will not be conducive to the analysis of components. The cells cultured for 0 days can be identified as the youngest hippocampal neurons, which will help us to explore the differential genes related to maturation in this study.…”

Section: Discussionmentioning

confidence: 99%

Deciphering the Heterogeneity of the Internal Environment of Hippocampal Neurons during Maturation by Raman Spectroscopy

et al. 2022

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Hippocampal neurons are sensitive to changes in the internal environment and play a significant role in controlling learning, memory, and emotions. A remarkable characteristic of the aging brain is its ability to shift from a state of normal inflammation to excessive inflammation. Various cognitive abilities of the elderly may suffer from serious harm due to the change in the neural environment. Hippocampal neurons may have various subsets involved in controlling their internal environment at different stages of development. Developmental differences may eventually result from complex changes in the dynamic neuronal system brought on by metabolic changes. In this study, we used an in vitro hippocampal neuron model cultured in C57BL/6J mice in conjugation with Raman spectroscopy to examine the relative alterations in potential biomarkers, such as levels of metabolites in the internal environment of hippocampal neurons at various developmental stages. The various differentially expressed genes (DEGs) of hippocampal neurons at various developmental stages were simultaneously screened using bioinformatics, and the biological functions as well as the various regulatory pathways of DEGs were preliminarily analyzed, providing an essential reference for investigating novel therapeutic approaches for diseases that cause cognitive impairment, such as Alzheimer’s disease. A stable hippocampal neuron model was established using the GIBCO C57BL/6J hippocampal neuron cell line as a donor and in vitro hippocampal neuron culture technology. The Raman peak intensities of culture supernatants from the experimental groups incubated for 0, 7, and 14 days in vitro(DIV) were examined. The GEO database was used to screen for different DEGs associated with various developmental stages. The data was then analyzed using a statistical method called orthogonal partial least squares discriminant analysis (OPLS-DA). The levels of ketogenic and glycogenic amino acids (such as tryptophan, phenylalanine, and tyrosine), lipid intake rate, glucose utilization rate, and nucleic acid expression in the internal environment of hippocampal neurons were significantly different in the 14 DIV group compared to the 0 DIV and 7 DIV groups ( P < 0.01). The top 10 DEGs with neuronal maturation were screened, and the results were compared to the OPLS-DA model’s analysis of the differential peaks. It was found that different genes involved in maturation can directly relate to changes in the body’s levels of ketogenic and glycogenic amino acids ( P < 0.01). The altered expression of the maturation-related genes epidermal growth factor receptor, protein tyrosine kinase 2-beta, discs large MAGUK scaffold protein 2, and Ras protein-specific guanine nucleotide releasing factor 1 may be connected to the altered uptake of ketogenic and glycogenic amino acids and nucleic acids in the internal environment of neurons at different developmental stages. The levels of ketogenic, glycogenic amino acids, and lipid intake ...

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“…Several cell lines employed for anti-Alzheimer's candidate discovery are SH-SY5Y primary cell line, CHO cell line, human neuroblastoma SK-N-SH cell line, primary cultured rat microglia and peripheral blood mononuclear cell (PBMC) from AD patients. SH-SY5Y cell line SH-SY5Y primary culture cells are humanderived cells that have been widely used in various in vitro experiments, including for anti-Alzheimer's discovery (Strother et al, 2021). Since derived from human cells, SH-SY5Y cells express human-specific protein and protein isoforms.…”

Section: N P R E S Smentioning

confidence: 99%

Curcumin Analogues as Novel Anti-Alzheimer's Candidates: Synthesis Development Strategy, In Vitro, Cell-Based and In Vivo Studies

Anas

Susidarti²,

Yuniarti³

et al. 2022

Indonesian J Pharm

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Alzheimer's disease (AD) is a neurological illness that causes a wide range of cognitive symptoms linked to amyloid plaque deposition, neurofibrillary tangles, oxidative stress, and neuron death in the whole brain. Curcumin has shown promising efficacy in preclinical studies for AD treatment. However, it failed to exhibit expected clinical outcomes in clinical studies. Besides, this molecule has low stability, solubility, and bioavailability properties. Hence, scientists have synthesized several curcumin analogues to improve their bioavailability and biological activity. The purpose of this narrative review is to discuss the development of curcumin analogue synthesis published in 2016-2021 and its efficacy that reveals its effect as an anti-Alzheimer's candidate through in vitro, cell-based and in vivo studies. Pubmed and Scopus database search engine with the keywords "curcumin" AND "analogues" OR "analogs" AND "Alzheimer's" were used to find the relevant studies. In our review, we include sixteen eligible journal articles to discuss. Fifteen curcumin analogues exhibited promise efficacy in preclinical studies and are suitable for development as anti-Alzheimer's candidates. Further study should explore to confirm the curcumin analogues toxicity, develop an appropriate dosage form, and initiate clinical trials.

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Long-term culture of SH-SY5Y neuroblastoma cells in the absence of neurotrophins: A novel model of neuronal ageing

Cited by 26 publications

References 61 publications

Vitamin B12 Attenuates Changes in Phospholipid Levels Related to Oxidative Stress in SH-SY5Y Cells

Vitamin B12 Attenuates Changes in Phospholipid Levels Related to Oxidative Stress in SH-SY5Y Cells

Deciphering the Heterogeneity of the Internal Environment of Hippocampal Neurons during Maturation by Raman Spectroscopy

Curcumin Analogues as Novel Anti-Alzheimer's Candidates: Synthesis Development Strategy, In Vitro, Cell-Based and In Vivo Studies

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