Long-term culture with 2,4-dichlorophenoxyacetic acid affects embryogenic competence in sugarcane callus via changes in starch, polyamine and protein profiles

Passamani, Lucas Zanchetta; Reis, Robert L.; Vale, Ellen Moura; Sousa, Kariane Rodrigues de; Aragão, Victor Paulo Mesquita; Santa–Catarina, Claudete; Silveira, Vanildo

doi:10.1007/s11240-019-01737-w

Cited by 25 publications

(14 citation statements)

References 80 publications

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“…The Spd content in R cell lines of P. koraiensis was significantly lower than that in B cell lines. In addition, some studies have revealed that with the long-term subculture of embryogenic calli, the potential for somatic embryogenesis is diminished, and it is easy to accumulate high levels of Put and Spd in embryogenic calli [ 11 ]. However, it was found in the verification experiment that there was a positive correlation between Spd content and SE yield, and the Spd content in embryogenic calli was significantly affected by genotype.…”

Section: Discussionmentioning

confidence: 99%

“…Studies have found that embryonic tissue with a large number of prominent immature SEs on the periphery has a high somatic embryogenesis potential, whereas embryonic tissue with a smooth outer periphery and almost no ESEs failed to produce SEs [ 10 ]. Furthermore, some research has analyzed calli with various somatic embryogenesis capacities and discovered that embryogenic calli with high somatic embryogenesis capacities have prominent nuclei, a high nucleus-to-cytoplasm ratio, small vacuoles, and other morphological characteristics [ 11 , 12 ]. Many studies, however, have demonstrated that the presence of ESEs in the embryonic tissue does not guarantee that the embryonic tissue may be utilized for somatic embryogenesis [ 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

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Morphological and Physiological Indicators for Screening Cell Lines with High Potential for Somatic Embryo Maturation at an Early Stage of Somatic Embryogenesis in Pinus Koraiensis

Peng

Gao

Wang

et al. 2022

Plants

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Many cell lines in the embryogenic callus cannot produce somatic embryos (SEs) even if they meet the optimal SE maturation culture conditions during conifer somatic embryogenesis. This phenomenon hinders the progress of the industrial-scale reproduction of conifers. Therefore, there is an urgent need to obtain morphological and physiological markers to screen embryogenic calli in response to SE maturation conditions. To detect cell lines with high somatic embryogenesis potential during the proliferation process, we counted the number of pro-embryos and early SEs (ESEs) in different cell lines and storage substances, endogenous hormones, and polyamine contents. The results showed that the yield of P. koraiensis SEs was heavily dependent on genotype (p = 0.001). There were high levels of PE III (pro-embryo III) number, ESE number, and soluble protein content, in the response cell lines (R cell lines), which were 1.6-, 3-, and 1.1-fold those of the obstructive cell lines (B cell lines), respectively. The B cell line had high levels of starch, auxin (IAA), Put, Spd, and putrescine: spermine (Put: Spm) compared to the R cell line. In addition, the numbers of PE III, ESEs, and soluble protein content were significantly positively correlated with SE yield. In contrast, the contents of starch, abscisic acid (ABA), Put, Spm, and Spd were significantly negatively correlated with SE yield. To ensure the accuracy of the results, we used nine cell lines to test the results. The PE III and ESE numbers and the Spm and Spd contents were positively correlated with SE yield, while the levels of starch, ABA, IAA, Put: Spd, and Put: Spm were negatively correlated with SE yield. Thus, we recommend using high PE III and ESEs as morphological indicators and low levels of starch, IAA, ABA, and Put: Spm as physiological markers to screen cell lines with a high somatic embryogenesis potential. In addition, we also found that the relationship between Spd, Spm, and SE yield was opposite in the two experimental results. Therefore, we speculate that the differences in Spd and Spm content are mainly affected by genotype. In conclusion, this study obtained the morphological and physiological markers of some high-somatic embryogenic cell lines by comparing the differences between nine somatic embryogenic cell lines. Our results can guide the improvement of conifer somatic embryogenesis technology and can provide a theoretical basis for accelerating the application of biotechnology in large-scale artificial breeding.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Morphological and Physiological Indicators for Screening Cell Lines with High Potential for Somatic Embryo Maturation at an Early Stage of Somatic Embryogenesis in Pinus Koraiensis

Peng

Gao

Wang

et al. 2022

Plants

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“…Researches on related biological mechanisms of reduced embryogenic potential showed that the changes in morphological characteristics can be easily detected by comparing morphological structures of different types of calli (Passamani et al 2020). Based on previous literature, long-term culture can modulate morphological changes in EC cells, which further interferes with the production of welldeveloped somatic embryos (Fu et al 2012;Passamani et al 2020). The study on sugarcane found that EC was smooth and dense, while NEC was brittle or soft and translucent (Silveira et al 2013).…”

Section: Relationship Between Morphological Characteristics and Embry...mentioning

confidence: 99%

“…Although to some extent, we observed morphological differences in different embryogenesis potential cultures of Korean pine, the control of endogenous hormones is likely through the appearance or disappearance of PEMs. The decrease in embryogenic potential during SE is a complex developmental process, which includes metabolism, gene expression, and a series of physiological changes (Passamani et al 2020). Therefore, further studies, including molecular studies, are required to better understand the complex mechanisms for decreasing embryogenic potential in Korean pine.…”

Section: )mentioning

confidence: 99%

Morphological and endogenous phytohormone changes during long-term embryogenic cultures in Korean pine

Liang

Shen

et al. 2022

Plant Cell Tiss Organ Cult

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Somatic embryogenesis (SE) is a biotechnological tool with high application potential for in vitro propagation and regeneration of conifers species. However, in SE of Korean pine, a dominant species of the natural mixed coniferous and broadleaf forests of the temperate forests in Northeast China, decreasing embryogenic potential was found in embryogenic callus (EC) with successive subcultures, especially when the cultures were maintained for more than 5-6 months, which is one of the most signi cant problems preventing commercial implementation of the SE technique. Aiming to investigate the morphological and the endogenous phytohormone changes associated with the decreasing embryogenic potential of Korean pine, here, we compared seven types of calli during long-term embryogenic culture. It was found that both the external morphological characteristics and internal cell structure were signi cantly different among the different types of calli. Speci cally, similar to their zygotic counterparts of conifer trees, the calli with high embryogenic potential were characterized by a higher number of designated proembryogenic masses (PEMs) structure. In addition, surface of this types of callus was covered with a large amount of mucilaginous extracellular matrix (ECM), which could further differentiate into globular embryos containing the embryonal masses (EM) and embryonic tube (ET) structure.In contrast, in those low embryogenic potential calli, we observed disintegrated PEMs structure, a large amount of irregularly shaped cells, and degraded cells, which failed to form somatic embryos. The endogenous hormone results showed that high levels of ABA were bene cial for promoting early somatic embryo differentiation in Korean pine, but maintaining high embryogenic potential required moderate levels of IAA and low levels of ZR. Additionally, a high ratio of IAA / ABA and MeJA / ABA was conducive to maintaining high embryogenic potential, whereas a low ratio of IAA / ABA and MeJA / ABA were effective for early somatic embryos morphological differentiation. This study demonstrates morphological and physiological characteristics of the embryogenesis potential of Korean pine calli.

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“…In addition to its frequent use for propagating various economically important species, embryogenic callus-based genetic transformation has emerged as the most successful plant transformation system in the last decade [2][3][4]. In plant tissue cultures, embryogenic callus formation is generally induced by optimizing the ratio of hormones in the medium [5][6][7]. Although hormones play central roles in embryogenic callus induction, this non-genetic method of optimizing culture conditions cannot provide culture schemes suitable for all genotypes [8,9].…”

Section: Introductionmentioning

confidence: 99%

Global Transcriptomic Analysis Reveals Differentially Expressed Genes Involved in Embryogenic Callus Induction in Drumstick (Moringa oleifera Lam.)

Yang

Zheng

Zhou

et al. 2021

IJMS

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The plant embryogenic callus (EC) is an irregular embryogenic cell mass with strong regenerative ability that can be used for propagation and genetic transformation. However, difficulties with EC induction have hindered the breeding of drumstick, a tree with diverse potential commercial uses. In this study, three drumstick EC cDNA libraries were sequenced using an Illumina NovaSeq 6000 system. A total of 7191 differentially expressed genes (DEGs) for embryogenic callus development were identified, of which 2325 were mapped to the KEGG database, with the categories of plant hormone signal transduction and Plant-pathogen interaction being well-represented. The results obtained suggest that auxin and cytokinin metabolism and several embryogenesis-labeled genes are involved in embryogenic callus induction. Additionally, 589 transcription factors from 20 different families were differentially expressed during EC formation. The differential expression of 16 unigenes related to auxin signaling pathways was validated experimentally by quantitative real time PCR (qRT-PCR) using samples representing three sequential developmental stages of drumstick EC, supporting their apparent involvement in drumstick EC formation. Our study provides valuable information about the molecular mechanism of EC formation and has revealed new genes involved in this process.

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Long-term culture with 2,4-dichlorophenoxyacetic acid affects embryogenic competence in sugarcane callus via changes in starch, polyamine and protein profiles

Cited by 25 publications

References 80 publications

Morphological and Physiological Indicators for Screening Cell Lines with High Potential for Somatic Embryo Maturation at an Early Stage of Somatic Embryogenesis in Pinus Koraiensis

Morphological and Physiological Indicators for Screening Cell Lines with High Potential for Somatic Embryo Maturation at an Early Stage of Somatic Embryogenesis in Pinus Koraiensis

Morphological and endogenous phytohormone changes during long-term embryogenic cultures in Korean pine

Global Transcriptomic Analysis Reveals Differentially Expressed Genes Involved in Embryogenic Callus Induction in Drumstick (Moringa oleifera Lam.)

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