Long‐term degradation from marshes into meadows shifts microbial functional diversity of soil phosphorus cycling in an alpine wetland of the Tibetan Plateau

Li, Meng; Hao, Yanbin; Yan, Zhongqing; Kang, Enze; Wang, Jinzhi; Zhang, Kerou; Li, Yong; Wu, Haidong; Kang, Xiaoming

doi:10.1002/ldr.4180

Cited by 21 publications

(12 citation statements)

References 40 publications

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“…Then, we clustered the protein sequences at 90% similarity level for each functional gene using CD‐HIT (v4.8.1) to remove redundancy, and gene diversity was calculated as the number of its non‐redundant protein sequences (Nelson et al, 2020; Wang, Li, Luo, et al, 2022). The taxonomic composition of N‐cycling microbial community was derived from count matrix, which was calculated by summing the relative abundance of taxa assigned to all N‐cycling genes detected at species levels for each sample (Li, Hao, Yan, et al, 2022). Functional and taxonomic α ‐diversities (i.e.…”

Section: Methodsmentioning

confidence: 99%

Asymmetric responses of abundance and diversity of N‐cycling genes to altered precipitation in arid grasslands

Zhao,

Shen,

Zhu

et al. 2023

Functional Ecology

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Precipitation changes exert a fundamental effect on the nitrogen (N) cycle in water‐limited grasslands. Soil microbes are essential drivers of N cycle, and the rates and their stabilities of interrelated N‐cycling processes are reflected by the abundance and diversity of N‐cycling genes. Yet, little is known about how altered precipitation affects the genes involved in the entire N‐cycling pathways. By combining a 6‐year precipitation manipulation experiment (−30%, ambient, +30%, +50%) with metagenomic sequencing, we investigated the responses of N‐cycling gene abundance and diversity to altered precipitation at two soil depths (0–10 and 30–50 cm). We found that increased precipitation enhanced the abundance of numerous key genes, leading to an acceleration of N turnover, but decreased the diversity of ammonium assimilation genes. Decreased precipitation did not reduce abundance or diversity of N‐cycling genes. Most N‐cycling genes showed generally consistent responses to altered precipitation in the topsoil (0–10 cm) and subsoil (30–50 cm), albeit with clear distinctions in both abundance and diversity by soil depth. These precipitation‐specific responses and depth‐dependent variabilities of functional genes were attributed to the distinct taxonomic composition of each N‐cycling gene. Furthermore, we quantified gross N transformation rates and found that they were well predicted by the abundance of most N‐cycling genes (e.g. genes involved in ammonium assimilation and nitrification). Our study sheds new light on the soil N cycle under precipitation alterations from the perspective of individual gene abundance and diversity and shows that future increases in precipitation could accelerate soil N turnover in arid and semi‐arid lands. Read the free Plain Language Summary for this article on the Journal blog.

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Section: Methodsmentioning

confidence: 99%

Asymmetric responses of abundance and diversity of N‐cycling genes to altered precipitation in arid grasslands

Zhao,

Shen,

Zhu

et al. 2023

Functional Ecology

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“…However, it was beyond our expectation that, in contrast to the other two P functional gene groups, we found that the abundances of P starvation response regulation genes were significantly higher in temperate forests than in those of the subtropics (Figure 2, Table S5). We suspect this could be attributable to soil P availability, given that this has a pronounced influence on the abundance of P starvation response regulation genes, such as phoR and phoB [11,36]. Under low AP conditions, microorganisms obtain P by regulating those genes involved in P starvation response regulation, thereby enhancing the release of P, which thus contributes to the efficient utilization of alternative P sources [10].…”

Section: P Functional Genes In Temperate and Subtropical Zonesmentioning

confidence: 99%

“…Increasing substrate availability (soil C) can potentially alleviate C limitation, thereby promoting a proliferation of microbial taxa [46], which in turn leads to N and/or P limitation in soils [47]. As a consequence of declining N and P availability, the activation of P starvation response regulation genes (mainly phoU, phoR, and phoB) [48] may enable the soil microbiota to exploit external or alternative P sources [11,20].…”

Section: Factors Affecting Soil P Functional Genes In Temperate and S...mentioning

confidence: 99%

“…P functional genes performs predominant roles in P cycling [8,9], with evidence indicating that microbial P cycling processes are mainly mediated by three main microbial gene groups [8,10,11], namely, P starvation response regulation, P uptake and transport, and P solubilization and mineralization genes. Among these, P solubilization and mineralization genes are typically the most common agents mediating the solubilization of mineral-bound inorganic P and mineralization of organic P [11]. The typical gene involved in solubilizing inorganic P is gcd, which coding for quinoprotein glucose dehydrogenase.…”

Section: Introductionmentioning

confidence: 99%

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Contrasting Soil Microbial Functional Potential for Phosphorus Cycling in Subtropical and Temperate Forests

Zhou

Wang

et al. 2022

Forests

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Microorganisms play important roles in phosphorus (P) cycling via their regulation of P uptake and transport, P mineralization and solubilization, and the mediation of P deficiency in forest biomes. However, the dynamics of microbial P functional genes and the underlying regulatory mechanisms in different forest biomes (e.g., temperate vs. subtropical) have yet to be sufficiently clarified. In this study, we applied a metagenomics approach to investigate changes in the abundance of three microbial P functional gene groups (P starvation response regulation genes, P uptake and transport genes, and P solubilization and mineralization genes) along a subtropical–temperate gradient of forest biomes (23° N–45° N) in China. Our results revealed that the abundances of P starvation response regulation genes in temperate forest biomes were significantly higher than those in the subtropics (p < 0.05), although not in the cases of the other two P functional gene types (p > 0.05). Moreover, in both temperate and subtropical forests, Acidobacteria, Actinobacteria, Proteobacteria, and Verrucomicrobia were identified as key phyla associated with P cycling; moreover, we found dominate species of Acidobacteria and Proteobacteria at genus level were higher in subtropical zones than that of temperate zones, in most cases. Furthermore, our results showed that significant correlation was found between P functional genes and microbial α-diversity along latitude gradient. Furthermore, in both forest biomes, microbial community α-diversity was significantly positively correlated with P starvation response regulation genes (p < 0.05), whereas α-diversity was significantly positively related to P uptake and transport genes in temperate forest biomes (p < 0.001), although not in subtropical forests (p > 0.05). In addition, we found that whereas soil substrates showed significant negative relationships with P solubilization and mineralization genes in temperate forest biomes (p < 0.05), this was not the case in subtropical forests. Collectively, these findings indicate that the responses of microbial P functional genes to the environmental variation in temperate forests are more sensitive than those in subtropical forests, thereby providing a theoretical foundation for further elucidation of the differential regulatory roles of these genes in different forest biomes.

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“…Generally, the soil is enriched in total P but lacks available P. In soil, 95% of P is unavailable (Paola et al, 2019). P is mostly bound to minerals or occurs in organic compounds, and therefore the amount of readily accessible P is a significant constraint on agricultural output (Gai et al, 2021; Li et al, 2022). Replenishment of soil P through fertilization is common in agriculture and forestry, but the long‐term sustainability of this practice is questionable (Azeez et al, 2020; Zhang, Zhang, et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Inoculation with phosphate‐solubilizing bacteria alters microbial community and activates soil phosphorus supply to promote maize growth

Sheng

Zheng

et al. 2022

Land Degrad Dev

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Phosphorus (P) is one of the essential macronutrients for plants, but agricultural soil generally lacks available P. This study aims to ascertain the impact of phosphatesolubilizing bacterial inoculation on the development of maize, soil characteristics, P fractions, and the microbial population in the soil. In this experiment, we explored the P solubilizing ability of P-solubilizing bacteria through liquid culture. In a maize pot experiment, soil P fractions and maize growth were examined, and a metagenomic sequence was constructed to analyze the soil microbial community and gene abundances and to link their relationship. In the shaking flask culture experiment, it was found that the Burkholderia (XQP35) and Raoultella (SQP80) have a strong capability for calcium phosphate and calcium phytate. Compared with the blank control, the XQP35 and SQP80 treatments could improve maize biomass and the soil nutrient status. Moreover, SQP80 treatments increased the soil acid phosphatase activity. SQP80 inoculation dramatically decreased the variety of the soil microbial community and the abundance of Acidobacteria. We found that the different treatments had no significant effect on the number of genes but that the SQP80 treatments significantly increased the P-cycling pathway gene family phoA and phoD compared with the CK treatment. Our results demonstrated that inoculation with XQP35 and SQP80 could dissolve varieties of insoluble P and, together with functional pathways of P-cycling, promote the transformation of P fractions and ultimately increase soil

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Long‐term degradation from marshes into meadows shifts microbial functional diversity of soil phosphorus cycling in an alpine wetland of the Tibetan Plateau

Cited by 21 publications

References 40 publications

Asymmetric responses of abundance and diversity of N‐cycling genes to altered precipitation in arid grasslands

Asymmetric responses of abundance and diversity of N‐cycling genes to altered precipitation in arid grasslands

Contrasting Soil Microbial Functional Potential for Phosphorus Cycling in Subtropical and Temperate Forests

Inoculation with phosphate‐solubilizing bacteria alters microbial community and activates soil phosphorus supply to promote maize growth

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