Long-term dynamics of dominant diatom species abundance of spring phytoplankton in three basins of the pelagic zone of Lake Baikal in 1964-1984 and 2007-2016

Usoltseva, Мarina; Titova, L.A.; Firsova, A.D.; Zakharova, Yulia

doi:10.31951/2658-3518-2023-a-2-55

Cited by 1 publication

(1 citation statement)

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“…U. acus is a dominant plankton species in the deepest freshwater lake on the planet, Lake Baikal, and thus one of the major primary producers in the lake's ecosystem [73,74]. Long-term studies of phytoplankton dynamics suggest that U. acus outcompetes other spring phytoplankton species [75], but it is unclear what exactly gives it an advantage.…”

Section: Of 21mentioning

confidence: 99%

Differential Expression of Stress Adaptation Genes in a Diatom Ulnaria acus under Different Culture Conditions

Bayramova,

Petrova,

Marchenkov

et al. 2024

IJMS

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Diatoms are a group of unicellular eukaryotes that are essential primary producers in aquatic ecosystems. The dynamic nature of their habitat necessitates a quick and specific response to various stresses. However, the molecular mechanisms of their physiological adaptations are still underexplored. In this work, we study the response of the cosmopolitan freshwater diatom Ulnaria acus (Bacillariophyceae, Fragilariophycidae, Licmophorales, Ulnariaceae, Ulnaria) in relation to a range of stress factors, namely silica deficiency, prolonged cultivation, and interaction with an algicidal bacterium. Fluorescent staining and light microscopy were used to determine the physiological state of cells under these stresses. To explore molecular reactions, we studied the genes involved in the stress response—type III metacaspase (MC), metacaspase-like proteases (MCP), death-specific protein (DSP), delta-1-pyrroline-5-carboxylate dehydrogenase (ALDH12), and glutathione synthetase (GSHS). We have described the structure of these genes, analyzed the predicted amino acid sequences, and measured their expression dynamics in vitro using qRT-PCR. We demonstrated that the expression of UaMC1, UaMC3, and UaDSP increased during the first five days of silicon starvation. On the seventh day, it was replaced with the expression of UaMC2, UaGSHS, and UaALDH. After 45 days of culture, cells stopped growing, and the expression of UaMC1, UaMC2, UaGSHS, and UaDSP increased. Exposure to an algicidal bacterial filtrate induced a higher expression of UaMC1 and UaGSHS. Thus, we can conclude that these proteins are involved in diatoms’ adaptions to environmental changes. Further, these data show that the molecular adaptation mechanisms in diatoms depend on the nature and exposure duration of a stress factor.

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Section: Of 21mentioning

confidence: 99%