Long-Term Effects of Acupuncture Treatment on Airway Smooth Muscle in a Rat Model of Smoke-Induced Chronic Obstructive Pulmonary Disease

Li, Jia; Wu, Song; Tang, Hongtu; Huang, Wei; Wang, Lushan; Zhou, Huanjiao Jenny; Zhou, Miao; Wang, Hua; Li, Jing

doi:10.1136/acupmed-2014-010674

Cited by 33 publications

(37 citation statements)

References 21 publications

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“…19 Acupuncture also significantly reduced the level of interleukin 8 (IL-8), a neutrophil chemotactic factor, and the pro-inflammatory cytokine tumour necrosis factor α (TNFα) in a mouse model of chronic obstructive pulmonary disease (COPD). 20 In this study, acupuncture at BL13 alone did not cause a significant reduction in the number of neutrophils. However, acupuncture at BL13+ST36 significantly reduced the number of neutrophils when compared with the OVA group.…”

Section: Inflammatory Responsementioning

confidence: 45%

Improvement in inflammation and airway remodelling after acupuncture at BL13 and ST36 in a mouse model of chronic asthma

et al. 2019

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Background: Asthma is a chronic inflammatory disease that affects millions of people worldwide. Chronic asthma is commonly resistant to steroid therapy. Acupuncture has an anti-inflammatory effect and has been widely used as an add-on therapy for asthma. Objective: To evaluate the effects of acupuncture on the inflammatory response and airway remodelling in the bronchioles of an asthma mouse model. Methods: A chronic asthma model was produced in female BALB/c mice by ovalbumin (OVA) sensitisation. 32 mice were randomised into four groups: control; asthma (OVA); OVA+BL13; and OVA+BL13+ST36. OVA was administered by intraperitoneal injection on days 0 and 14 followed by aerosol exposure of 1% OVA three times a week for 6 weeks. Manual acupuncture (MA) was performed three times a week for 6 weeks at BL13 alone, or BL13 in combination with ST36, in the two MA-treated groups. At the end of the experiment, blood samples were collected to determine eosinophil and neutrophil counts and lung tissue was prepared for histological examination. Results: A pronounced reduction in the neutrophil count was achieved after MA at BL13+ST36 (P=0.005) while the eosinophil count was lowered after MA both at BL13 (P=0.007) and BL13+ST36 (P=0.006). Reduction in the bronchiolar epithelial and smooth muscle thickness and the number of goblet cells was observed after MA at BL13 (P=0.001, P=0.001 and P=0.002, respectively) and BL13+ST36 (P=0.001, P=0.002 and P=0.001, respectively). Conclusion: Acupuncture can reduce the inflammatory response and prevent airway remodelling in a chronic asthma mouse model.

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Section: Inflammatory Responsementioning

confidence: 45%

Improvement in inflammation and airway remodelling after acupuncture at BL13 and ST36 in a mouse model of chronic asthma

et al. 2019

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“…The electroacupuncture treatment was carried out as follows: the EA, BY + EA, and SA rat groups were mildly anaesthetized abdominally with 10% chloral hydrate at 2 mL/kg to ensure that all rats completely recovered from anesthesia within approximately 30 min [35]. Dazhui (GV 14, located on the posterior midline below the spinous process of the seventh cervical vertebra), Feishu (BL 13, located below and 3 mm lateral to the third thoracic vertebra on the back, bilateral), and Shenshu (BL 23, located below and 3 mm lateral to the second lumbar vertebra on the waist, bilateral) were selected as the acupoints [44] (Figure 1(a)).…”

Section: Copd Model Preparation Andmentioning

confidence: 99%

“…Acupuncture has been used as a clinical treatment for COPD for thousands of years [35]. Based on the "meridian theory" described in the Emperor's Classic of Internal Medicine, acupuncture could regulate Qi and dredge meridians to treat various diseases [36].…”

Section: Introductionmentioning

confidence: 99%

“…In the treatment of COPD, acupuncture showed clinical effects in improving the exercise capacity and respiratory function and reducing dyspnea during exercise, especially in severe patients [39,40]. Animal experiments have suggested that acupuncture could contribute to lung protection by regulating inflammatory cytokines in a smokeinduced COPD rat model [35,41].…”

Section: Introductionmentioning

confidence: 99%

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Effect of Bufei Yishen Granules Combined with Electroacupuncture in Rats with Chronic Obstructive Pulmonary Disease via the Regulation of TLR-4/NF-κB Signaling

Tian

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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Background. The combined therapy of Bufei Yishen granules (BY) and electroacupuncture (EA) has shown good effects clinically in treating chronic obstructive pulmonary disease (COPD). The present study aimed to observe the effects of the BY + EA combination in a COPD rat model and dissect the potential mechanisms via Toll-like receptor (TLR) 4/nuclear factor kappa B (NF-κB) signaling. Methods. The COPD rats were treated with normal saline, aminophylline, Bufei Yishen granules, electroacupuncture, or Bufei Yishen granules combined with electroacupuncture. The pulmonary function; lung tissue histology; levels of inflammatory factors; expression levels of TLR-4, inhibitor of nuclear factor kappa B (IκB), and NF-κB; and activation of NF-κB in the lung tissues were evaluated. Results. Pulmonary function was markedly decreased in the COPD rats, and the lung tissue histology of the COPD rats showed severe pathological changes. The pulmonary function and lung tissue morphology in the treatment groups (APL, BY, EA, and BY + EA) were improved. The increased levels of the inflammatory cytokines interleukin (IL)-1β and IL-6 indicated a chronic inflammatory state in the COPD rats. In the BY, EA, and BY + EA groups, the levels of IL-1β and IL-6 were decreased, especially in the BY + EA group. In addition, the mRNA and protein expression levels of TLR-4, IκB, and NF-κB were obviously downregulated in the BY and BY + EA groups; and the NF-κB p65 activation was significantly decreased in the BY, EA, and BY + EA groups. Conclusions. Bufei Yishen granules and electroacupuncture have curative effects in COPD rats, and the combination therapy of Bufei Yishen granules and electroacupuncture is superior. The TLR-4/NF-κB pathway may be involved in the potential mechanisms by which Bufei Yishen granules and electroacupuncture reduce inflammation.

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“…Therefore, it has been regarded as an intractable disease around the world. As stated in the article by Li et al, long time acupuncture treatment had a positive effect on airway smooth muscle of chronic obstructive lungs (Li et al 2016). In analog to acupuncture, moxibustion is a commonly used therapy in Traditional Chinese Medicine.…”

Section: Introductionmentioning

confidence: 95%

Moxibustion Has a Positive Effect on Pulmonary Fibrosis: An Alternative Approach

Cheng¹,

Li²,

Mei³

et al. 2017

Afr J Tradit Complement Altern Med

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Background: An increasing number of people suffered idiopathic fibrosis (IPF) and the current treatment was far from clinical satisfaction. Moxibustion, another effective and safe unconventional therapy, had been introduced to treat this refractory disease. The study aimed to investigate the effect of moxibustion on a bleomycin A5-induced pulmonary fibrosis model. Materials and Methods: Sprague-dawley (SD) rats were randomly allocated to the blank group, model group, moxibustion group, and prednisone group, for which they received no treatment, modeling, moxibustion treatment and prednisone treatment. After four-week treatment, the rats were euthanized for Hematoxylin and Eosin (H.E.) staining, and TGF-β1 and IFN-γ protein and mRNA detection in lungs.Results: In the model group, TGF-β1 was significantly increased and IFN-γ was significantly decreased at both protein and mRNA levels in comparison to the blank group. In the moxibustion and prednisone group, however, TGF-β1 was decreased and IFN-γ was increased at both protein and mRNA levels in comparison to the model groups. Compared with prednisone, moxibustion showed comparable effect in lowing TGF-β1 (P>0.05) and better effect in up-regulating IFN-γ(P>0.05). Conclusion:The study concludes moxibustion protected pulmonary fibrosis by downregulating TGF-β1 and upregulating IFN-γ cytokines at both mRNA and protein levels, and the effect was comparable to prednisone. Moxibustion could be used as a therapeutic alternative treatment for pulmonary fibrosis.

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Long-Term Effects of Acupuncture Treatment on Airway Smooth Muscle in a Rat Model of Smoke-Induced Chronic Obstructive Pulmonary Disease

Cited by 33 publications

References 21 publications

Improvement in inflammation and airway remodelling after acupuncture at BL13 and ST36 in a mouse model of chronic asthma

Improvement in inflammation and airway remodelling after acupuncture at BL13 and ST36 in a mouse model of chronic asthma

Effect of Bufei Yishen Granules Combined with Electroacupuncture in Rats with Chronic Obstructive Pulmonary Disease via the Regulation of TLR-4/NF-κB Signaling

Moxibustion Has a Positive Effect on Pulmonary Fibrosis: An Alternative Approach

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