Long-term effects of dietary isoflavones on uterine gene expression profiles

Möller, Frank; Zierau, Oliver; Hertrampf, Torsten; Bliedtner, Anja; Diel, Patrick; Vollmer, Günter

doi:10.1016/j.jsbmb.2009.01.016

Cited by 20 publications

(15 citation statements)

References 45 publications

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“…As has been thoroughly discussed in the literature, both in vitro and in vivo studies have shown that GEN and EQ are known to be the strongest amongst the isoflavones in ER␤ binding [28][29][30]57]. Moreover, receptor binding assays revealed that GEN fits very well into the ER␤ ligand-binding pocket, and therefore exhibits a strong binding affinity for ER␤ [58].…”

Section: Discussionmentioning

confidence: 89%

“…Those have been previously studied in our lab and with collaborators in an ER␣-dependent test systems in vitro and in animal experiments in vivo [25,28,33,[53][54][55][56].…”

Section: Discussionmentioning

confidence: 99%

“…Subsequently, the microbial flora partially breaks the aglycones to further metabolites; specifically GEN to dihydrogenistein and DAI to Equol (EQ) (Fig. 1c) [27,28]. GEN and DAI in addition to EQ are known to possess estrogenic properties [29][30][31].…”

Section: Introductionmentioning

confidence: 99%

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Activation of transgenic estrogen receptor-beta by selected phytoestrogens in a stably transduced rat serotonergic cell line

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Section: Discussionmentioning

confidence: 89%

“…Those have been previously studied in our lab and with collaborators in an ER␣-dependent test systems in vitro and in animal experiments in vivo [25,28,33,[53][54][55][56].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Activation of transgenic estrogen receptor-beta by selected phytoestrogens in a stably transduced rat serotonergic cell line

Amer

Kretzschmar

Müller

et al. 2010

The Journal of Steroid Biochemistry and Molecular Biology

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“…Moreover, a group of female 172 wild-type littermates (WT; n = 6) with the same genetic background (C57BL/6J) were 173 included in the experimental setup as a reference group for physiologically normal 174 mice. Mice were maintained on isoflavone-free diet, because it has been recently 175 shown from our laboratory team, that long-term intake of dietary phytoestrogens 176 influences the estrogenic responses in the organism (Moller et al, 2009). 177…”

Section: Treatment and Experimental Design 168mentioning

confidence: 99%

Effects of genistein and estrogen receptor subtype-specific agonists in ArKO mice following different administration routes

Bliedtner

Zierau

Albrecht

et al. 2010

Molecular and Cellular Endocrinology

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30We have scrutinized the effects of the phytoestrogen genistein and three synthetic 31 estrogen receptor agonists, 17α-ethynylestradiol (EE), propylpyrazole-triol (PPT) and 32 diarylpropionitrile (DPN) in the completely estrogen-free background of aromatase 33 knockout (ArKO) mice by means of two routes of substance administration: oral via 34 diet (per os; po) or subcutaneous injection (sc) with the intention to evaluate the 35 ArKO mice as sensitive model organism for uterotrophic assays. Additionally, we 36 were aiming to qualitatively analyze effects resulting from oral administration path, in 37 particular for PPT and DPN. Therefore, we analyzed the resulting uterine wet weights 38 (UWW) and epithelial heights as physiological endpoints of function as well as the 39 gonadotropin levels. Moreover, the gene expression profiles of estrogen receptors as 40 well as important uterine and ovarian estrogen-response genes were investigated by 41The uterus of ArKO mice responded very sensitive upon the substitution with EE (sc 43 5 µg/ kg BW; po 50 µg/ kg BW) in a proliferative manner. This was evaluated inter 44 alia by increased UWW and by up-regulation of the expression of proliferation-45 associated and estrogen-response genes. It is important to note, that ERα and ERβ-46 agonist, PPT and DPN respectively (po 5 mg / kg BW and sc 0.5 mg / kg BW), so far 47 have only been just for sc applications. Here, effects resulting from oral application 48 were qualitatively described evaluated for their applicability. The UWW and 49 expression of proliferation-associated genes were increased following both po and sc 50 treatment with PPT. DPN did not exert an increase of the UWW, but a significant 51 decrease of proliferation-associated gene and protein expression. Additionally, a 52 substantial hypoplasia was detectable in the uterine cross sections of DPN-treated 53 mice. On the other hand, the phytoestrogen genistein (sc 10 mg/ kg BW; po 70 54 mg/ kg BW) did not cause detectable uterotrophic responses or large changes of 55 uterine and ovarian gene expression profiles under the applied experimental 56conditions, but significantly reduced the elevated gonadotropin levels of ArKO mice. 57In summary, we showed the utility of ArKO mice to detect ER-specific effects, in 58 particular those of PPT and DPN also when applied orally. 59 60

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“…For example, the known cause of infertility in female mice that consumed the flavonoid genistein could be that uteri of genistein-treated females are not capable of supporting normal implantation (Jefferson et al, 2009). In fact, maternal genistein consumption is able to alter uterine wet weight and gene expression in the offspring, in which, a particularly striking change is observed in the expression of the estrogenic marker complement-C3 gene in juveniles (Moller et al, 2009).…”

Section: A Proposed Mechanism For Endocrine-mediated Flavonoid Actionmentioning

confidence: 99%

We are what we Eat: How Nutritional Compounds Such as Isoflavones Shape Our Epigenome

Guerrero-Bosagna

Clark

2011

Nutrition in Epigenetics

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Long-term effects of dietary isoflavones on uterine gene expression profiles

Cited by 20 publications

References 45 publications

Activation of transgenic estrogen receptor-beta by selected phytoestrogens in a stably transduced rat serotonergic cell line

Activation of transgenic estrogen receptor-beta by selected phytoestrogens in a stably transduced rat serotonergic cell line

Effects of genistein and estrogen receptor subtype-specific agonists in ArKO mice following different administration routes

We are what we Eat: How Nutritional Compounds Such as Isoflavones Shape Our Epigenome

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