Long-term evaluation of oral gavage with periodontopathogens or ligature induction of experimental periodontal disease in mice

Abstract

The ligature model was an effective approach to induce inflammation and bone loss similar to human periodontitis, but the oral gavage models were not efficient in inducing periodontal inflammation and tissue destruction in the conditions studied. Ligature models can provide a basis for future interventional studies that contribute to the understanding of the disease pathogenesis and the complex host response to microbial challenge.

Epigenetic and inflammatory events in experimental periodontitis following systemic microbial challenge

“…In this study, it is important to note that the experimental periodontitis was conducted in C57BL/6 mice that are considered a less susceptible for the oral gavage model of periodontitis induction (Graves et al, ). Hence, we modified the protocol and introduced P. gingivalis multiple times during the procedure of oral gavage than previously reported (de Molon et al, ). As observed, by increasing the period of microbial challenge, our method successfully promoted significant bone loss, inflammatory and epigenetic changes both at the local and at the systemic level.…”

Early intervention of peri‐implantitis and periodontitis using a mouse model

“…Researchers have been able to induce experimental periodontal bone loss in animal models through oral gavage of specific pathogens, lipopolysaccharide injections, and through the ligation of foreign materials around teeth . By applying these techniques to implants in animal models, studies have been able to induce some level of experimental peri‐implant bone loss .…”

“…To test our hypothesis, we employed a split‐mouth ligature murine model to evaluate and compare the response of acute induced (early‐stage) peri‐implantitis and periodontitis after removal of the noxious stimuli (ligature). The use of ligatures is a well‐established model to induce inflammation given its ability to harbor periodontopathogens . Therefore, clinically, ligature removal represent the removal of bacteria harboring agents or conditions that lead to inflammation, such as calculus, cement, and overcontoured restorations, to name a few.…”

Effects of quorum‐sensing inhibition on experimental periodontitis induced by mixed infection in mice

“…Porphyromonas gingivalis and F. nucleatum were allowed to interact for 5 min and the inocula were individually prepared in disposable 1‐ml needleless syringes (Kovax‐Syringe 1 ml; Korea Vaccine, Ansan, Korea) for each mouse. After direct harvest of viable P. gingivalis and F. nucleatum from culture suspension, a duration of 30 min was necessary to prepare the inocula for oral gavage.…”