Long-term Experimental Hypodynamy Affects the Structure of Spongy Bone and Osteoclasts in Japanese Quails

Zibrín, M; Bodă, K; Cigánková, Viera; Kocisová, J; Tomajková, E.; Komorová, T.; Sabo, V.; Pivko, J.

doi:10.2754/avb200372020143

Cited by 5 publications

(6 citation statements)

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“…As an animal avian model, Japanese quail is often used in studies of the toxicology of chemical compounds and the effects of environmental endocrine disruptors or in examining the physiological processes in birds [11][12][13]. Quails are also used as an animal model for studying bone formation and development, both in pre-hatch and post-hatch studies [14][15][16][17][18][19][20][21].…”

Section: Introductionmentioning

confidence: 99%

The effect of bee pollen on bone biomechanical strength and trabecular bone histomorphometry in tibia of young Japanese quail (Coturnix japonica)

et al. 2020

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It has been demonstrated in numerous studies that bee pollen supplementation shows numerous positive effects on health. However, its impact on bones is largely unknown. The purpose of this study was to investigate the effect of bee pollen supplementation on the tibia biomechanical properties and bone morphometric measures using Japanese quail as an animal model. The experiment was arranged in a 2x2x2 factorial design, with sex, quail line (meat-type or egg-lying type), and bee pollen inclusion (0 or 10 g/kg of feed) as factors. The quails were one-day-old at the beginning of the experiment, they were euthanized after 42 days. Our study showed for the first time unfavorable effects of bee pollen on bones properties. Bee pollen supplementation negatively affected bone structure, irrespective of quails' sex or line type. Bone length (P < 0.001), weight (P < 0.01), and mean relative wall thickness (P < 0.01) and mineralization (P < 0.05) were reduced by bee pollen treatment. For female quails, irrespective of line type, the decrease of yield load (P < 0.001), ultimate load (P < 0.01), yield stress (P < 0.001) and ultimate stress (P < 0.05) was noted. Analysis of growth plate in bone metaphysis showed that bee pollen supplementation slowed the process of bone maturation irrespective of sex (P < 0.05). On contrary, dietary bee pollen positively affected bone homeostasis of trabecular bone in bone metaphysis as bone mineral density increased in experimental groups (P < 0.05). In males, this was the result of the increase of trabecular thickness (P < 0.01), in females due to the reduction of trabecular space (P < 0.001). In conclusion, our results demonstrate that bee pollen (1.0%, 10 g/kg of feed) supplementation caused significant negative effects on the mechanical endurance of the tibia of quails, while showed beneficial effects on trabecular bone histomorphometry.

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Section: Introductionmentioning

confidence: 99%

The effect of bee pollen on bone biomechanical strength and trabecular bone histomorphometry in tibia of young Japanese quail (Coturnix japonica)

et al. 2020

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“…In our study, we made use of the Japanese quail as an animal model. This species has been routinely used as an animal model for in vivo studies on bone development across various disciplines within the biological sciences for decades [32][33][34], which makes it an optimal model for a study on the probiotic regulation of bone health. Our study was performed on both male and female quail, and to avoid the effects of egg production on bone homeostasis in hens (i.e., formation of medullary bone), our study was performed on young, rapidly growing quail that did not lay eggs.…”

Section: Discussionmentioning

confidence: 99%

“…Bone density measurements have been widely used to assess bone mechanical endurance [37]. However, the quality of the collagen within the bone and the bone diaphysis geometric indices also provide important information concerning bone strength [33,34]. In bending, bone load-carrying capacity and the bone's resistance to an imposed bending load, directly depends on the area moment of inertia about the bending axis for a specific cross-section of the bone diaphysis, for a particular bone [26].…”

Section: Discussionmentioning

confidence: 99%

Effects of Yeast (Saccharomyces Cerevisiae) Probiotics Supplementation on Bone Quality Characteristics in Young Japanese Quail (Coturnix Japonica): The Role of Sex on the Action of the Gut-Bone Axis

Muszyński

Dobrowolski

Kasperek

et al. 2020

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The aim of the study was to investigate the changes in bone geometry, histological structure, and selected mechanical characteristics in young male and female Japanese quails supplemented with Saccharomyces cerevisiae. Quails were fed a basal diet containing no yeast or a basal diet supplemented with 1.5% (15 g per 1 kg of diet) of inactive S. cerevisiae, for a period of 42 days. S. cerevisiae inclusion had no effect on bone weight, length, and density, diaphysis geometry (cross-sectional area, wall thickness, moment of inertia) or on the mechanical strength (yield load, ultimate load, stiffness, Young’s modulus, yield stress, ultimate stress). Yeast supplementation improved the morphology of the articular cartilage both in male and female quails, as the total thickness of the articular cartilage was significantly increased. In trabecular bone, an increase in real bone volume and trabecular thickness was observed in females supplemented with S. cerevisiae, while in males the increase in trabecular number was accompanied by a reduction in trabecular thickness. The results of the present study demonstrate that S. cerevisiae, through a sex-dependent action on the gut-bone axis, improved the structure of articular cartilage and microarchitecture of trabecular bone. The positive effects of S. cerevisiae supplementation were more evident in female quails.

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“…This study follows previous research on the Japanese quail bones conducted by Zibrin et al (2003) with different periods of hypodynamy. Bones are not the only one tissue affected by weightlessness.…”

Section: Discussionmentioning

confidence: 99%

Influence of long-term hypodynamy on spongy bone tissue in Japanese quails

et al. 2013

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Weightlessness can cause various damages especially on the musculoskeletal system both in animals and humans. The aim of our study was to observe the influence of simulated, long-term microgravity on the spongy bone tissue of the femur in Japanese quails. A total of 80 cockerels at the age of 2 days were exposed to simulated microgravity -hypodynamy. After days 56, 63, 90 and 180, six birds from the experimental group and six birds from the control group were euthanised. Samples for histological examination were collected from femur epiphysis. The whole femur of the other limb was used for the analysis of the calcium content. Microscopic examination showed differences between experimental and control animals in the spongy bone tissue after every day of the experiment. In the experimental animals, there were numerous, big, multinucleated cells osteoclasts, lying on the bone trabeculae surface, which were damaged. The highest difference in the calcium content in femurs between the control and experimental animals was found after 90 days of hypodynamy. This study builds on short-term hypodynamy experiments; such long periods had never been studied before in birds. Because our findings are similar to those found in osteoporotic bone tissue, it could by useful in the development of countermeasures against the negative influence of microgravity and immobilization. Microgravity, morphology, osteoporosis, osteoclastsOsteoporosis is a systemic skeletal disease characterized by low bone mass and microarchitectural deterioration of bone tissue, with a consequent increase in bone fragility and susceptibility to fracture (WHO 1994). Among the well known pathological conditions that can lead to development of osteoporosis are the absence of motion and weightlessness. That is why osteoporosis in connection with muscular atrophy occurs in immobilized patients and was also found in cosmonauts after longterm space missions.In order to find out effective countermeasures to prevent the development of osteoporosis, it is necessary to know the cytological mechanism of how these changes develop, and to focus on the morphological changes in defected tissues. Experiments under real microgravity in space are demanding and very expensive, so several models for simulated microgravity on Earth -hypodynamy were created, using experimental animals such as birds, rats and monkeys. Japanese quails are small, unexacting birds with low body weight, fast individual development and high tolerance to difficult conditions, good reproduction and high production (Baumgartner and Hetenyi 2001). Thanks to their good adaptability to microgravity they are great experimental animals for simulated microgravity (Skrobanek and Hrancova 2003). In future they might be used in space shuttles during long-term space flights as producers of animal proteins for astronauts (Boda 1984(Boda , 1997.In this work we focused on structural and ultrastructural changes in spongy bone tissue in Japanese quails and their development during different periods of long-term ...

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Long-term Experimental Hypodynamy Affects the Structure of Spongy Bone and Osteoclasts in Japanese Quails

Cited by 5 publications

References 33 publications

The effect of bee pollen on bone biomechanical strength and trabecular bone histomorphometry in tibia of young Japanese quail (Coturnix japonica)

The effect of bee pollen on bone biomechanical strength and trabecular bone histomorphometry in tibia of young Japanese quail (Coturnix japonica)

Effects of Yeast (Saccharomyces Cerevisiae) Probiotics Supplementation on Bone Quality Characteristics in Young Japanese Quail (Coturnix Japonica): The Role of Sex on the Action of the Gut-Bone Axis

Influence of long-term hypodynamy on spongy bone tissue in Japanese quails

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