Long-term exposure of zebrafish to bisphenol F: Adverse effects on parental reproduction and offspring neurodevelopment

Gu, Jie; Li, Liangzhong; Yin, Xiaogang; Liang, Mengyuan; Zhu, Yuanhui; Guo, Min; Zhou, Li; Fan, Deling; Shi, Lili; Ji, Guixiang

doi:10.1016/j.aquatox.2022.106190

Cited by 20 publications

(10 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, this change of nrd mRNA transcription was like that of elavl3 . These findings were in agreement with the results of Gu et al, which found that both nrd and elavl3 were downregulated by bisphenol F exposure and further impacted the differentiation and development of neurons. Overall, this finding showed that 3-BCZ exposure inhibited the development of CNS neurons by disrupting the differentiation of newborn neurons in zebrafish.…”

Section: Resultssupporting

confidence: 93%

“…Swimming behavior is an important indicator of behavioral response during the early life stages of zebrafish and has been widely used to test the neurotoxicity of various environmental contaminants. 34 Total swimming distance as the most representative parameter of swimming behavior was evaluated in this study. After exposing zebrafish larvae to 3-BCZ for 120 hpf, the total swimming distances were recorded as shown in Figure 3.…”

Section: Impacts Of 3-bcz On the Behavior Activities Of Zebrafish At ...mentioning

confidence: 99%

See 1 more Smart Citation

3-Bromocarbazole-Induced Developmental Neurotoxicity and Effect Mechanisms in Zebrafish

et al. 2023

View full text Add to dashboard Cite

Polyhalogenated carbazoles (PHCZs) are dioxinlike emerging contaminants, but limited information exists on their neurotoxicity and mechanisms. This study employed zebrafish embryos to investigate the adverse effects of 3-bromocarbazole (3-BCZ), a typical PHCZ, exposure in their early life stages and found that the 120 h post fertilization (hpf)-median lethal concentration (LC 50 ) value of 3-BCZ in zebrafish larvae was 2.88 mg/L. The results revealed that 3-BCZ inhibited tail coiling at ≥0.06 mg/L, decreased hatchability at ≥2.88 mg/L, increased mortality and malformation rates at ≥1.44 mg/L, induced aryl hydrocarbon receptor effects and reduced body length at ≥0.58 mg/L, and inhibited behavior activities at ≥0.06 mg/L. Further mechanism investigations showed that 3-BCZ exposures repressed the motor neuron axon length via downregulating the mRNA transcription levels of α1-tubulin, Gap43, Syn2a, and shha, and decreased central nervous system neurogenesis via downregulating the mRNA transcription levels of elavl3, nrd, mbp, and gfap. Overall, 3-BCZ induces developmental neurotoxicity in zebrafish larvae at 0.06 mg/L (1/50 LC 50 ). Tail coiling activity at 24 hpf and swimming total distance at 120 hpf could be ideal indicators of 3-BCZ health risks in zebrafish. These findings provide valuable insights for health and ecological risk assessments of 3-BCZ and other PHCZs.

show abstract

Section: Resultssupporting

confidence: 93%

Section: Impacts Of 3-bcz On the Behavior Activities Of Zebrafish At ...mentioning

confidence: 99%

3-Bromocarbazole-Induced Developmental Neurotoxicity and Effect Mechanisms in Zebrafish

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Generally, the toxicity of substitutes should be weaker than that of the original; however, studies have shown that BPs may have higher toxicity than BPA [ 14 , 15 ]. Several studies have shown that environmentally relevant concentrations of BPA and its analogs may cause abnormalities in mammalian or fish cell viability [ 16 ], growth [ 17 ], the digestive system [ 18 ], the nervous system [ 19 ], vascular development [ 20 ], and reproduction [ 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Developmental and Reproductive Impacts of Four Bisphenols in Daphnia magna

Qian

Chen

Guo

et al. 2022

IJMS

View full text Add to dashboard Cite

Bisphenol A (BPA) is a typical endocrine-disrupting chemical (EDC) used worldwide. Considering its adverse effects, BPA has been banned or strictly restricted in some nations, and many analogs have been introduced to the market. In this study, we selected three representative substitutes, BPS, BPF, and BPAF, along with BPA, to assess the developmental and reproductive effects on Daphnia magna. The F0 generation was exposed to bisphenols (BPs) at an environmentally relevant concentration (100 μg/L) for 21 d; then the embryo spawn at day 21 was collected. Behavior traits, the activity of antioxidant enzymes, and gene transcription were evaluated at three developmental stages (days 7, 14, and 21). Notably, body length, heart rate, and thoracic limb beating were significantly decreased, and D. magna behaved more sluggishly in the exposed group. Moreover, exposure to BPs significantly increased the antioxidant enzymatic activities, which indicated that BPs activated the antioxidant defense system. Additionally, gene expression indicated intergenerational effects in larvae, particularly in the BPAF group. In conclusion, BPA analogs such as BPF and BPAF showed similar or stronger reproductive and developmental toxicity than BPA in D. magna. These findings collectively deepen our understanding of the toxicity of BPA analogs and provide empirical evidence for screening safe alternatives to BPA.

show abstract

“…Studies have shown that increased expression of BCL-2 slows down apoptosis and increases the time to cell death (63). Some studies have found a decrease in BCL-2 expression after bisphenol treatment, such as Gu et al who treated zebrafish with BPF and found their spawn to have downregulated BCL-2 mRNA expression (61). On the other hand, studies have also demonstrated an increase BCL-2 mRNA expression following bisphenol treatment, as detected by Peretz et al in cultured murine antral follicles (10).…”

Section: Discussionmentioning

confidence: 99%

“…Similar upregulations in expression were also found in ovarian cells, murine sperm, and the ovaries of mice offspring treated with BPA ( 58 – 60 ). When it comes to BPF, caspase-9 mRNA was increased in zebrafish larvae and in macrophages ( 54 , 61 ). Caspase-9 is an important component of the intrinsic pathway of apoptosis and the upregulation of this caspase provides us with more evidence that apoptosis induced by BPA and BPF occurs through the intrinsic pathway.…”

Section: Discussionmentioning

confidence: 99%

Bisphenols A and F, but not S, induce apoptosis in bovine granulosa cells via the intrinsic mitochondrial pathway

Kourmaeva

Sabry²,

Favetta³

2022

Front. Endocrinol.

View full text Add to dashboard Cite

With the gradual decline in global fertility rates, there is a need to identify potential contributing factors, their mechanisms of actions and investigate possible solutions to reverse the trend. Endocrine disrupting compounds (EDCs), such as bisphenol A (BPA), are environmental toxicants that are known to negatively impact reproductive functions. As such, the use of BPA in the manufacturing industry has slowly been replaced by analogs, including bisphenol S (BPS) and bisphenol F (BPF), despite limited knowledge available regarding their impact on health and their safety. The following study investigates the effects of BPA, BPS and BPF at a concentration of 0.5 μg/mL and 50 μg/mL on bovine granulosa cell apoptosis, with the ultimate goal of determining how they may impact oocyte competence and, thus, overall fertility. The underlying hypothesis is that bisphenols disrupt the granulosa cell environment surrounding the oocyte inducing excessive apoptosis via the intrinsic mitochondrial pathway. To test this hypothesis, apoptosis was measured following a time- and dose-dependent exposure to all three bisphenols by flowcytometry paired with annexin V/PI staining as well as by quantification of key genes belonging to the intrinsic apoptotic pathway both at the mRNA and protein levels. The results of this study report that BPA and BPF reduce cell viability through reduced cell counts and increased apoptosis. This increase is due, in part, to the induction of apoptotic genes of the intrinsic pathway of apoptosis. Additionally, this study also suggests that BPS may not act on the intrinsic mitochondrial apoptotic pathway in bovine granulosa cells. Overall, this study allows us to establish potential apoptotic pathways activated by bisphenols as well as compare the relative apoptotic activities of BPA to its most widespread analogs.

show abstract

Long-term exposure of zebrafish to bisphenol F: Adverse effects on parental reproduction and offspring neurodevelopment

Cited by 20 publications

References 45 publications

3-Bromocarbazole-Induced Developmental Neurotoxicity and Effect Mechanisms in Zebrafish

3-Bromocarbazole-Induced Developmental Neurotoxicity and Effect Mechanisms in Zebrafish

Developmental and Reproductive Impacts of Four Bisphenols in Daphnia magna

Bisphenols A and F, but not S, induce apoptosis in bovine granulosa cells via the intrinsic mitochondrial pathway

Contact Info

Product

Resources

About