Long-term follow-up of a supradescemetic keratoprosthesis in rabbits: an immunofluorescence study

Espana, Edgar M.; Acosta, A.C.; Stoiber, J.; Fernández, Viviana; Lamar, P.; Villain, Franck; Lacombe, E; Alfonso, Eduardo C.; Parel, Jean Marie

doi:10.1007/s00417-010-1497-4

Cited by 6 publications

(8 citation statements)

References 18 publications

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“…The results of the in vivo biocompatibility evaluations conducted in this work are further strengthened by the fact that the rabbit eye is generally more sensitive than the human eye [39]. The rabbit cornea is thinner with a steeper curvature and is more susceptible to evaporative damage because of a slower blink frequency than a human eye [38].…”

Section: Discussionmentioning

confidence: 93%

“…Another factor strengthening our findings is that topical corticosteroids were given only for a few days postoperatively; standard clinical postoperative regimens might have masked inflammation in our rabbit experiments. In addition, the rabbit eye readily extrudes foreign material, limiting long-term studies beyond a few months [39]. Thus we believe that the present work is an important step toward testing these HMPEI-coated materials in humans.…”

Section: Discussionmentioning

confidence: 99%

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Biocompatibility and biofilm inhibition of N,N-hexyl,methyl-polyethylenimine bonded to Boston Keratoprosthesis materials

et al. 2011

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The biocompatibility and antibacterial properties of N,N-hexyl,methyl-polyethylenimine (HMPEI) covalently attached to the Boston Keratoprosthesis (B-KPro) materials was evaluated. By means of confocal and electron microscopies, we observed that HMPEI-derivatized materials exert an inhibitory effect on biofilm formation by Staphylococcus aureus clinical isolates, as compared to the parent poly(methyl methacrylate) (PMMA) and titanium. There was no additional corneal epithelial cell cytotoxicity of HMPEI-coated PMMA compared to that of control PMMA in tissue cultures in vitro. Likewise, no toxicity or adverse reactivity was detected with HMPEI-derivatized PMMA or titanium compared to those of the control materials after intrastromal or anterior chamber implantation in rabbits in vivo.

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Biocompatibility and biofilm inhibition of N,N-hexyl,methyl-polyethylenimine bonded to Boston Keratoprosthesis materials

et al. 2011

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“…In addition, investigation of different forms of graphene (e.g., graphene oxide, reduced graphene oxide, and graphene-decorated flexible surfaces) with modified mechanical properties, as well as long-term in vivo behaviors of different graphene materials, are warranted to obtain a better understanding of its potential in clinical application. 51,52 Our initial findings have demonstrated great promise for GBMs in the development of ophthalmologic biomaterials.…”

Section: Discussionmentioning

confidence: 99%

Application of Graphene as Candidate Biomaterial for Synthetic Keratoprosthesis Skirt

Tan

Thompson

Konstantopoulos

et al. 2015

Invest. Ophthalmol. Vis. Sci.

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PURPOSE. Synthetic keratoprostheses are required for visual rehabilitation in patients with endstage corneal blindness. This study aimed to assess the biocompatibility of graphene material and its potential as a novel synthetic keratoprosthesis skirt material for corneal tissue engineering. METHODS.Human corneal stromal fibroblasts were cultured on material surfaces including pristine graphene film, graphene foam, pristine titanium (Ti) discs, and tissue culture plastic surface (TCPS). Cell attachment was assayed by immunostaining of paxillin and vinculin. Cell viability and proliferation were assessed by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and Click iT 5-ethynyl-2 0 -deoxyuridine (EdU) assays. The growth of fibroblasts on three-dimensional graphene foam was examined by scanning electron microscopy, and cytokine release was analyzed by enzyme-linked immunosorbent assay. Graphene films were implanted into rabbit corneal stromal pockets and examined by slit-lamp biomicroscopy, anterior segment optical coherence tomography, in vivo confocal microscopy, and histology.RESULTS. Pristine graphene demonstrated good biocompatibility with human stromal fibroblasts in terms of cell adhesion, viability, and proliferation. Cells on graphene films showed higher number than on TCPS control. Cells grown on graphene had 10% more proliferation than on Ti. The expression levels of IL-6 and IL-8 were reduced when cells were seeded on graphene foam as compared to Ti and graphene film. Implantation of graphene film into rabbit stroma (n ¼ 6) did not show any signs of infection, neovascularization, or inflammation.CONCLUSIONS. Graphene displayed excellent short-term biocompatibility with corneal cells and tissue. This demonstrates that graphene can be developed as a tissue engineering material for use in cornea.

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“…Besides these facts, according to Pressler (2010), corneal allografts have still shown the best results in keratoplasty, however, they have limited supply and may not be readily available. Thus, biopolymers are becoming more studied in replacement of corneal tissue to retrieve the appropriate refractive status of the eye (Espana et al 2011). To be applied to the cornea, a material must be transparent, allowing epithelial growth on its surface, should not trigger an exaggerated immune response and should allow fibroplasia and deposition of extracellular matrix in the interface (Tanure 2006).…”

Section: Introductionmentioning

confidence: 99%

Bacterial cellulose and bacterial cellulose/polycaprolactone composite as tissue substitutes in rabbits' cornea

et al. 2016

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In order to test the performance of bacterial cellulose/polycaprolactone composite (BC/PCL) and pure bacterial cellulose (BC) as tissue substitutes in rabbits' cornea, a superficial ulcer containing 5mm in diameter and 0.2mm deep was made in the right cornea of 36 rabbits, then a interlayer pocket was created from the basis of this ulcer. Twelve rabbits received BC/PCL membrane and 12 were treated with BC membranes, both membranes with 8mm in diameter. The remaining rabbits received no membrane constituting the control group. The animals were clinically followed up for 45 days. Three animals of each group were euthanized at three, seven, 21, and 45 days after implantation for histological examination of the cornea along with the implant. Clinical observation revealed signs of moderate inflammatory process, decreasing from day 20th in the implanted groups. Histology showed absence of epithelium on the membranes, fibroplasia close to the implants, lymph inflammatory infiltrate with giant cells, collagen disorganization, with a predominance of immature collagen fibers in both groups with implants. Although inflammatory response is acceptable, the membranes used does not satisfactorily played the role of tissue substitute for the cornea during the study period.

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Long-term follow-up of a supradescemetic keratoprosthesis in rabbits: an immunofluorescence study

Cited by 6 publications

References 18 publications

Biocompatibility and biofilm inhibition of N,N-hexyl,methyl-polyethylenimine bonded to Boston Keratoprosthesis materials

Biocompatibility and biofilm inhibition of N,N-hexyl,methyl-polyethylenimine bonded to Boston Keratoprosthesis materials

Application of Graphene as Candidate Biomaterial for Synthetic Keratoprosthesis Skirt

Bacterial cellulose and bacterial cellulose/polycaprolactone composite as tissue substitutes in rabbits' cornea

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