Long-Term Functional End Points Following Middle Cerebral Artery Occlusion in the Rat

Nogo Receptor Antagonism Promotes Stroke Recovery by Enhancing Axonal Plasticity

“…Adult male Sprague Dawley rats (Charles River Laboratories, Wilmington, MA) weighing 250 -350 gm were used. Left middle cerebral artery occlusion was induced with a intraluminal filament (Longa et al, 1989;Belayev et al, 1996;Hudzik et al, 2000). Anesthesia was induced with 5% isoflurane and maintained with 2% isoflurane in an oxygen/air mixture using Fluovac Scavenger (Stoelting).…”

A Master Key to Assess Stroke Consequences Across Species: The Adhesive Removal Test

“…Correlations between the cortical and striatal histological lesions and the ischemia-induced behavioral impairments in the adhesive removal test have been well investigated in the literature, mostly in rodents (Grabowski et al, 1991;Hudzik et al, 2000;Hunter et al, 1998;Rogers et al, 1997;Virley et al, 2000). We and other authors have demonstrated a close correlation between contralateral contact and removal latencies on this task and abnormal changes in the ipsilateral caudate putamen, lower parietal cortex and forelimb cortex following transient MCAo (30; 60 or 90 min; see Figure 5).…”

Improvements of the Stroke Model Guidelines Animal body weight and long-term functional concerns

“…In the same way of thoughts, whereas the correlation between acute histological lesion and early behavioral impairment is well documented (Rogers et al 1997), less is known about the long-term evolution of this relationship. Moreover, those correlation studies have to take into account the different brain structures affected by stroke in order to bring a better understanding of their involvement in behavioral impairments (Grabowski et al 1993;Hudzik et al 2000). Basically, direct and indirect methods are unanimously admitted for estimating lesion volume both in the whole brain or for each cerebral structure.…”