Long‐Term Intake of Pork Meat Proteins Altered the Composition of Gut Microbiota and Host‐Derived Proteins in the Gut Contents of Mice

Abstract

Scope This study is to investigate the effects of long‐term intake of pork protein on the composition of gut microbiota and proteins in mice. Methods and results C57BL/6J mice are fed pork meat protein diets for 240 days, and the composition of gut microbiota and proteins in luminal contents from the duodenum to the colon are analyzed by 16S rRNA gene sequencing and LC‐MS/MS. The stewed pork protein diet group has a highly similar microbiota composition to that of the cooked pork protein diet group, but different from the pork emulsion sausage or dry‐cured pork protein diet groups. Lachnospiraceae NK4A136, Odoribacter, Defluviitaleaceae UCG‐011, Ruminiclostridium 9, Blautia, Lachnoclostridium, and Ruminococcaceae UCG‐010 play an important role in response to changes in gut luminal proteins. Specific microbes are significantly correlated with the Cela3b and Cpa1 that are derived from the host and involve protein digestion and absorption. Conclusions Pork meat protein diets alter the gut microbiota composition and specific gut microbes may have a great impact on protein digestion and absorption by regulating the secretion of digestive proteins from the host. These findings provide a new insight into the associations of long‐term intake of meat protein diet with gut microbiota and host.

Effect of stocking density and age on physiological performance and dynamic gut bacterial and fungal communities in Langya hens

“…The Methanobrevibacter genus, which contained the most abundant methanogenic archaea in animals, increased energy absorption efficiency by cooperating with hydrogen-producing Hydrogenoanaero microorganisms highly enriched in the obese human gut [ 70 ]. Defluviitaleaceae UCG-011 was reported to play a major part in response to alterations in intestinal luminal proteins [ 71 ]. At the same time, Oscillibacter was involved in the utilization of carbohydrates and proved to increase rumen fermentation in calves [ 72 ].…”

“…The increased proportion of inflammation-related bacterium ( Oscillospiraceae ) and Erysipelotrichaceae_UCG-003 were linked to the sub-health status in the host [ 62 , 81 ]. Xie et al [ 71 ] documented that Lachnoclostridium had a crucial role in response to variations in intestinal proteins, while Eisenbergiella was reported to be connected with weight gain in humans [ 66 ]. These data conveyed the message that the colonization of the gut microbiome is a constantly changing and non-fixed ecosystem influenced by the factors such as stocking density and age.…”

Body weight index indicates the responses of the fecal microbiota, metabolome and proteome to beef/chicken-based diet alterations in Chinese volunteers

“…Total peptide signals within each run were normalized before quantitation. Afterwards, the counts of peptides between samples were compared to obtain a matrix of protein ratios, calculated as the median of all ratios for peptides 44 . Protein abundance was calculated on the basis of the normalized spectral protein intensity (LFQ intensity).…”

“…Protein abundance was calculated on the basis of the normalized spectral protein intensity (LFQ intensity). OmicsBean ( www.omicsbean.cn ) was used to analyze the selected proteome data, in which GO analysis and KEGG pathway analysis were conducted 44 .…”

Reconsidering Meat Intake and Human Health: A Review of Current Research

“…[109,110] In a study investigating long-term dietary intervention, consumption of different processed pork products at the recommended dosage changed the composition of the gut microbiota, but did not cause serious inflammation. [111] However, consuming some pork products could cause problems for immune deficient individuals with impaired innate immune systems. The study indicated that administration of processed meat proteins had limited influence on the physiology and gut health of wild-type mice, but a significant negative impact on Rag1-/-mice (adaptive immune-deficient mice).…”