Long-term luminescence of organic dyes in cells of biological tissues

Letuta, S. N.; Maryakhina, Valeriya S.; Pashkevich, S. N.; Рахматуллин, Р Р

doi:10.1134/s0030400x11010103

Cited by 10 publications

(3 citation statements)

References 5 publications

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“…The rate constant of TDF of dye located in the biomaterial k TDF is significantly less than the value for samples with cells (table 1). This hypothesis is confirmed by the results of the previous investigations [13]. It is experimentally shown that the DF kinetics of xanthene dye does not change with air pressure variation over the biomaterial.…”

Section: Results and Their Discussionsupporting

confidence: 86%

Pathology development stage and its influence on the delayed fluorescence kinetics of molecular probes

Maryakhina

Letuta

2013

Laser Phys.

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This paper describes regularity of the delayed fluorescence kinetics changes of xanthene dyes in the cancer cells received from tumors of different diameter. It shows that the rate constant of bimolecular reactions (singlet-triplet T− 1 g (O 2 ) and triplet-triplet annihilations) as well as thermoactive fluorescence decrease with increasing pathological process development stage. It could be a result of both more expressed cancer cell hypoxia and a change in cytoplasm viscosity.

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Section: Results and Their Discussionsupporting

confidence: 86%

Pathology development stage and its influence on the delayed fluorescence kinetics of molecular probes

Maryakhina

Letuta

2013

Laser Phys.

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“…It is seen that the phosphorescence kinetics of erythrosine is the most sensitive to the initial concentration of dye. We note that the phosphorescence kinetics of these dyes located in mammary cells is also biexponential [15]. The obtained differences can be described by immobilization of monomers and dimers of the used dyes.…”

Section: Resultsmentioning

confidence: 86%

Concentration of photosensitizers as a factor impacting on the results of fluorescent diagnostics

Maryakhina¹,

Letuta²

2014

Laser Phys.

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This paper describes the dependence of the spectral-luminescent properties of photosensitizers on their concentration. It has been shown that the initial concentration of the injected photosensitizer or probe can impact on the result of fluorescent diagnostics, in particular on laser flash photolysis and fluorimetric analysis results. Differences of optical measurements have been seen using cancer cells obtained from malignant tumors with different diameters (1-3 cm). These results can be used for the development of optical methods for biomedical diagnostics, for example photosensitizer dose selection.

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“…29,33 DF kinetics recorded macroscopically from cancerous tissue treated with xanthene dyes have been proposed as a potential diagnostic and dosimetric tool for PDT. [34][35][36][37] SOSDF can be accompanied by other types of delayed fluorescence (DF) of the PS, 34,35 namely thermally-activated delayed fluorescence (thermDF)…”

Section: Introductionmentioning

confidence: 99%

The singlet-oxygen-sensitized delayed fluorescence in mammalian cells: a time-resolved microscopy approach

Scholz

Biehl

Dědic

et al. 2015

Photochem Photobiol Sci

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The present work provides a proof-of-concept that the singlet oxygen-sensitized delayed fluorescence (SOSDF) can be detected from individual living mammalian cells in a time-resolved microscopy experiment. To this end, 3T3 mouse fibroblasts incubated with 100 μM TPPS4 or TMPyP were used and the microsecond kinetics of the delayed fluorescence (DF) were recorded. The analysis revealed that SOSDF is the major component of the overall DF signal. The microscopy approach enables precise control of experimental conditions - the DF kinetics are clearly influenced by the presence of the (1)O2 quencher (sodium azide), H2O/D2O exchange, and the oxygen concentration. Analysis of SOSDF kinetics, which was reconstructed as a difference DF kinetics between the unquenched and the NaN3-quenched samples, provides a cellular (1)O2 lifetime of τΔ = 1-2 μs and a TPPS4 triplet lifetime of τT = 22 ± 5 μs in agreement with previously published values. The short SOSDF acquisition times, typically in the range of tens of seconds, enable us to study the dynamic cellular processes. It is shown that SOSDF lifetimes increase during PDT-like treatment, which may provide valuable information about changes of the intracellular microenvironment. SOSDF is proposed and evaluated as an alternative tool for (1)O2 detection in biological systems.

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Long-term luminescence of organic dyes in cells of biological tissues

Cited by 10 publications

References 5 publications

Pathology development stage and its influence on the delayed fluorescence kinetics of molecular probes

Pathology development stage and its influence on the delayed fluorescence kinetics of molecular probes

Concentration of photosensitizers as a factor impacting on the results of fluorescent diagnostics

The singlet-oxygen-sensitized delayed fluorescence in mammalian cells: a time-resolved microscopy approach

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