Long‐term NaHS administration reduces oxidative stress and apoptosis in a rat model of left‐side varicocele

Lorian, Keivan; Kadkhodaee, Mehri; Kianian, Farzaneh; Abdi, Arash; Ranjbaran, Mina; Ashabi, Ghorbangol; Seifi, Behjat

doi:10.1111/and.13496

Cited by 14 publications

(9 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Please note that groups with common 'a' are also significantly different from each other only for sperm motility and not for sperm abnormal morphology Micheli et al, 2019) which includes reduced tubule differentiation index, spermiation index, the diameter of seminiferous tubes, Johnson score as well as sperm parameters and sperm functional tests. These results are in keeping with our previous studies and several other studies have explained that due to the increased testicular heat stress in varicocele conditions and subsequent increased oxidative stress, abnormal sperm chromatin packaging and DNA fragmentation are among the common pathological feature of varicocele (Asadi et al, 2018;Lorian et al, 2020;Razi et al, 2021).…”

Section: Discussionsupporting

confidence: 93%

Effect of curcumin, and nano‐curcumin on sperm function in varicocele rat model

et al. 2021

View full text Add to dashboard Cite

One of the main and acceptable causes of infertility in men is 'varicocele' due to the abnormal enlargement and dilation of the testicular veins (especially the left one), the pathological consequences of which are testicular damage and reduced sperm quality (Jensen et al., 2017). According to the literature background, several mechanisms could account for these pathological consequences including hypoxia, increased testicular temperature, changes in testicular blood flow and venous pressure, stasis, inflammation, decreased gonadotropins and androgens, oxidative stress (OS) and apoptosis.Most of these factors can be related to an increase in testicular temperature, the consequence of which is an increase in oxidative stress that can have detrimental effects on cell and tissue health. Oxidative stress occurs as a result of either increased production of reactive oxygen species (ROS) or reduced antioxidant production capacity

show abstract

Section: Discussionsupporting

confidence: 93%

Effect of curcumin, and nano‐curcumin on sperm function in varicocele rat model

et al. 2021

View full text Add to dashboard Cite

show abstract

“…In our study, NaHS administration did not change body weight; this finding is in line with other studies in male mice 22 , 23 and male rats 18 ; however, two studies reported that NaSH restored reduced body weight in nephrectomized rats 24 , 26 and other studies did not report an effect of NaSH administration on body weight 15 – 17 , 19 – 21 , 25 . In addition, in our study, NaSH administration did not affect serum urea and Cr, a finding that aligns with another report 25 .…”

Section: Discussionsupporting

confidence: 92%

“…Despite evidence that NaHS solutions are not stable, some animal studies used NaHS solutions in drinking water as an H 2 S-donating compound 15 – 26 , with the duration of intervention ranging from 1 to 21 weeks (Table 2 ). These studies refreshed NaHS solutions every 12 15 , 17 , 18 , 24 , 25 or 24 19 – 23 h. Our results indicate that due to H 2 S loss from the NaHS solution, rats are not exposed to a stable concentration of the drug, and a large fluctuation in the content of NaHS in the drinking water of rats occurs over 12 or 24 h (see Fig. 2 ).…”

Section: Discussionmentioning

confidence: 65%

“…Seven studies have reported that they used NaHS from Sigma 15 , 16 , 19 – 23 but did not provide more details about water of hydration, and five studies did not mention the source of NaHS used for their preparations 17 , 18 , 24 – 26 . NaHS is a hydrated molecule and may have variable waters of hydration, affecting the amount of NaHS needed for preparing a given molar concentration of the solution.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Dissolving sodium hydrosulfide in drinking water is not a good source of hydrogen sulfide for animal studies

Ghasemi,

Jeddi,

Yousefzadeh

et al. 2023

Sci Rep

View full text Add to dashboard Cite

Hydrogen sulfide (H2S) has many physiological and pathological roles in the human body. Sodium hydrosulfide (NaHS) is widely used as a pharmacological tool for assessing H2S effects in biological experiments. Although H2S loss from NaHS solution is a matter of minutes, some animal studies use NaHS in solution as an H2S-donating compound in drinking water. This study addresses whether 30 μM NaHS in drinking water prepared in rat/mouse water bottles remains stable for at least 12–24 h, as presumed by some authors. NaHS solutions (30 μM) were prepared in drinking water and immediately transferred to rat/mice water bottles. Samples were obtained from the tip of water bottles and from inside of the bottles at 0, 1, 2, 3, 4, 5, 6, 12, and 24 h for sulfide measurement using the methylene blue method. Furthermore, NaHS (30 μM) was administered to male and female rats for two weeks, and serum sulfide concentrations were measured every other day in the first week and at the end of the second week. NaHS solution was unstable in the samples obtained from the tip of water bottles; it declined by 72% and 75% after 12 and 24 h, respectively. In the samples obtained from the inside of the water bottles, the decline in the NaHS was not significant until 2 h; however, it decreased by 47% and 72% after 12 and 24 h, respectively. NaHS administration did not affect serum sulfide levels in male and female rats. In conclusion, NaHS solution prepared in drinking water can not be used for H2S donation as the solution is unstable. This route of administration exposes animals to variable and lower-than-expected amounts of NaHS.

show abstract

“…The interventional or surgical approach is considered the standard of care for the condition in case of infertility due to favourable outcomes regarding improvement in semen parameters and pregnancy rates (2). In modern era, research has also focused on the varicocele-induced oxidative stress and reduction in antioxidant capacity which results in impaired spermatogenesis, broadening new horizons in the treatment of the condition (3,4). Antioxidative treatment is well-established in idiopathic infertility and oligoasthenoteratospermia (OAT), albeit the results still conflicting and the optimal agents or combination still lacking (5).…”

Section: Introduction -Backgroundmentioning

confidence: 99%

L-carnitine as primary or adjuvant treatment in infertile patients with varicocele. A systematic review

Tsampoukas

Khan

Katsouri

et al. 2020

Arch Ital Urol Androl

View full text Add to dashboard Cite

Background: Varicocele has been found to impair the function of the epididymis resulting in subfertility whereas the varicocelectomy can resolve the phenomenon. L-carnitine is regarded as a biomarker for the function of the epididymis and has been found in reduced concentrations in infertile patients of various causes, including infertile men with varicocele. It seems that Lcarnitine and varicocele share clinical significance and the area of research looks promising. Objective: To identify the role of L-carnitine in the treatment of varicocele. Materials and methods: A systematic search was performed in Pubmed/Medline with the terms (L-carnitine) and (varicocele) and (L-carnitine) and (varicocelectomy). Inclusion criteria were studies reported outcomes of L-carnitine administration alone or in duet, as primary or adjuvant treatment to varicocele. Exclusion criteria were non-English language and animal studies. Studies using L-carnitine as part of a panel of therapeutic agents were avoided. Results: Only four suitable studies were identified for discussion. In one randomized study, the combination of L-carnitine and cinnoxicam improved semen parameters in patients with non-high-grade varicocele compared to L-carnitine alone and had a favourable effect on pregnancy rates but the effect of grade is unknown. In another study, as an adjuvant treatment to varicocelectomy, L-carnitine showed no clear benefit. Finally, in comparison to surgery, the results are inconclusive; two studies showed some benefit might be expected in low-grade or subclinical varicocele, but surgery appears superior. Conclusions: The evidence regarding the role of L-carnitine as a primary or adjuvant treatment of varicocele is sparse. The pathophysiological significance of L-carnitine implicates a potential role of the molecule in the management of varicocele, but the evidence so far is controversial for any recommendations. L-carnitine might be taken into consideration in selected cases; however, further search is needed in order the optimal role of L-carnitine in infertile patients with varicocele to be clarified.

show abstract

Long‐term NaHS administration reduces oxidative stress and apoptosis in a rat model of left‐side varicocele

Cited by 14 publications

References 30 publications

Effect of curcumin, and nano‐curcumin on sperm function in varicocele rat model

Effect of curcumin, and nano‐curcumin on sperm function in varicocele rat model

Dissolving sodium hydrosulfide in drinking water is not a good source of hydrogen sulfide for animal studies

L-carnitine as primary or adjuvant treatment in infertile patients with varicocele. A systematic review

Contact Info

Product

Resources

About