Long-term stimulation of mouse hippocampal slice culture on microelectrode array

Bergen, A van; Papanikolaou, T.; Schuker, A; Möller, Andreas; Schlosshauer, Bürkhard

doi:10.1016/s1385-299x(03)00024-2

Cited by 46 publications

(29 citation statements)

References 27 publications

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“…The non-invasive nature of this technique permits real-time analysis of long-term effects that are often difficult to be assessed by traditional electrophysiological techniques [12][13][14][15][16][17] .…”

Section: Introductionmentioning

confidence: 99%

Neural circuits and temporal plasticity in hindlimb representation of rat primary somatosensory cortex: revisited by multi-electrode array on brain slices

Wang

Chang

et al. 2010

Neurosci. Bull.

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Objective The well-established planar multi-electrode array recording technique was used to investigate neural circuits and temporal plasticity in the hindlimb representation of the rat primary somatosensory cortex (S1 area).Methods Freshly dissociated acute brain slices of rats were subject to constant perfusion with oxygenated artificial cerebrospinal fluid (95% O 2 and 5% CO 2 ), and were mounted on a Med64 probe (64 electrodes, 8×8 array) for simultaneous multi-site electrophysiological recordings. Current sources and sinks across all the 64 electrodes were transformed into twodimensional current source density images by bilinear interpolation at each point of the 64 electrodes. Results The local intracortical connection, which is involved in mediation of downward information flow across layers II-VI, was identified by electrical stimulation (ES) at layers II-III. The thalamocortical connection, which is mainly involved in mediation of upward information flow across layers II-IV, was also characterized by ES at layer IV. The thalamocortical afferent projections were likely to make more synaptic contacts with S1 neurons than the intracortical connections did. Moreover, the S1 area was shown to be more easily activated and more intensively innervated by the thalamocortical afferent projections than by the intracortical connections. Finally, bursting conditioning stimulus (CS) applied within layer IV of the S1 area could successfully induce long-term potentiation (LTP) in 5 of the 6 slices (83.3%), while the same CS application at layers II-III induced no LTP in any of the 6 tested slices. Conclusion The rat hindlimb representation of S1 area is likely to have at least 2 patterns of neural circuits on brain slices: one is the intracortical circuit (ICC) formed by interlaminar connections from layers II-III, and the other is the thalamocortical circuit (TCC) mediated by afferent connections from layer IV. Besides, ICC of the S1 area is spatially limited, with less plasticity, while TCC is spatially extensive and exhibits a better plasticity in response to somatosensory afferent stimulation. The present data provide a useful experimental model for further studying microcircuit properties in S1 cortex at the network level in vitro.

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Section: Introductionmentioning

confidence: 99%

Neural circuits and temporal plasticity in hindlimb representation of rat primary somatosensory cortex: revisited by multi-electrode array on brain slices

Wang

Chang

et al. 2010

Neurosci. Bull.

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“…We examined the concentration required to reach 50% of the maximal effect (EC 50 ) on mean spike rate in the neuronal networks for a series of compounds and compared these values to the known oral and/or intraperitoneal (IP) dose that produces lethality in 50% of cases (LD 50 ; Table 1). A strong positive correlation exists between the sensitivity of the neuronal networks as determined by log EC 50 and the toxicity of the compound, represented by log LD 50 ( Figure 4). These data suggest that the prediction capacity for the log LD 50 values from neuronal network results is ~±2 log units.…”

Section: Broadband Sensitivitymentioning

confidence: 97%

“…For example, Cd induces significant alterations in neuronal network activity at ~1 μM. If we apply the rat oral LD 50 for CdCl 2 of 88 mg/kg for a 70 kg human, a molecular weight of 183 g/mol, and a conservative estimate of 10 L of water consumption, the LD 50 for Cd corresponds to 4.8 mM. The cultured neuronal network shows a significant response well below these levels, which suggests potential for environmental threat detection.…”

Section: Broadband Sensitivitymentioning

confidence: 99%

Broadband Detection of Environmental Neurotoxicants

Pancrazio¹

2007

Anal. Chem.

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“…The low recording probability of single unit activity in tissue placed onto substrate integrated planar microelectrodes depends on the fact (Egert et al, 1998) that only the activity of neurons from the lower part of a tissue is detected which is separated from the array by a layer of either dead cells in acute slices or glia in organotypic cultures (Del Rio et al, 1991;Coltman and Ide, 1996). As expected the proportion of microelectrodes in a planar array detecting bioelectric activity, in particular in neocortical slices or cultures, is higher (>80%) in the case of synchronized, high amplitude population activities (van Bergen et al, 2003;Beggs and Plenz, 2004;Heck et al, 2008). The probability of recording single unit activities might be increased by using three-dimensional substrate integrated microelectrode arrays (Thiebaud et al, 1997; 3D-MEA from <www.multichannelsystems.com>).…”

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confidence: 94%