Long-term storage and viability of aquatic oomycetes

Clark, Gillian; Dick, M.W.

doi:10.1016/s0007-1536(74)80116-6

Cited by 21 publications

(3 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…was obtained from the mycology collection of Embrapa Amazônia Oriental in Belém, Pará, and was used in all the tests. The isolate was preserved according to the method of Castellani, considered the most suitable for oomycetes (CLARK & DICK, 1974). For the experiment, the isolate was plated on PDA medium (200 g potato, 20 g agar and 20 g dextrose per liter of autoclaved distilled water).…”

Section: Methodsmentioning

confidence: 99%

Culture medium and inoculation methodology for the study of soft root rot caused by Phytopythium sp.

et al. 2019

View full text Add to dashboard Cite

Cassava (Manihot esculenta Crantz) is an important crop in Brazil and Pará is the major producer of roots. High temperature and humidity of tropical regions favor the development of various diseases, among them the cassava root rot. The objective of this study was to evaluate the effect of luminosity and culture medium on the mycelial growth and sporulation of Phytopythium sp. associated with different methods of inoculation on cassava roots. In vitro tests for pathogen growth were established in a 2 x 6 factorial design (luminosity x culture medium) with five replicates and the means were compared by t test (P≤0.05). The culture medium containing sweet cassava root produced greater mycelial development and higher pathogen sporulation and it was the most suitable medium for pathogen culture. The culture under absence of light generated better mycelial growth than culture under 12 hour of light. Regarding the type of inoculation, the response was better when deeper injuries were induced.

show abstract

Section: Methodsmentioning

confidence: 99%

Culture medium and inoculation methodology for the study of soft root rot caused by Phytopythium sp.

et al. 2019

View full text Add to dashboard Cite

show abstract

“…The following species were collected between 12 December 1985 and 26 May 1987, from the Whiteknights Lake area, University of Reading: Achlya racemosa (Clark & Dick, 1974) were used for preparation of axenic cultures.…”

Section: Methodsmentioning

confidence: 99%

Biometry of oogonia and oospores of Saprolegniaceae

Soumati

Dick

1989

Botanical Journal of the Linnean Society

View full text Add to dashboard Cite

SOUMATI, B. & DICK, M. W., 1989. Biometry of oogonia and oospores of Saprolegniaceae. The distribution of data on the dimensions of oogonia and oospores for modal and near modal classes of oospore number per oogonium in multiovulate Oomycetes has been determined. Thirteen species of Saprolegniaceae have been examined. Two fundamental relationships have been identified: the first relationship is that of common intercept line regression between the oogonium volume and the mean volume of oospores, the second relationship is that of parallel line regression between the oogonium volume and the sum of volumes of oospores. Neither the quality of water nor the cultural temperature had a significant effect on the pattern of the two relationships. The general pattern of these relationships was the same for all species and with all modal classes tested. Achlya species had higher intercepts in the first relationship and lower slopes in the second relationship compared with Saprolegnia species. The species of hoachlya examined occupied a more intermediate position. The fact that the dimensional relationships are significantly different for different classes of oospore number per oogonium means that these statistics must be considered when writing descriptions of species. The use of overall dimensional ranges embracing all oospore number classes may obscure taxonomic discontinuities.

show abstract

“…From stock axenic cultures (Clark & Dick, 1974;Dick, 1965) an agar plug was transferred to a Petri plate containing V8 broth (2.5%, v/v, V8 juice), and allowed to grow at room temperature into a thick mycelial mat. The mat was transferred to 500 ml V8 broth in a 1 litre flask, shaken vigorously and the contents dispensed into Petri plates.…”

Section: Species Pythium Diclinummentioning

confidence: 99%

Comparison of Physical Maps of Ribosomal DNA Repeating Units in Pythium, Phytophthora and Apodachlya

Klassen¹,

McNabb²,

Dick³

1987

Microbiology

View full text Add to dashboard Cite

~ ~~sometimes branched, while that of P. torulosum is contorted into distinctive toruloid swellings.Pythium irregulare seldom produces sporangia, but when they are formed they are small (about 1750 pm3), spherical, and terminal or intercalary. Pythium anandrum produces large (about 47 500 pm3) ellipsoid sporangia that sometimes show internal renewal, and has spiny oogonia. Pythium paddicum has sporangia of intermediate dimensions and has papillate oogonia. Thus, the five species can be regarded as spanning the range of sporangial diversity within the genus.Abbreviation: rDNA, ribosomal DNA. 0001-3936 Q 1987 SGM

show abstract

Long-term storage and viability of aquatic oomycetes

Cited by 21 publications

References 1 publication

Culture medium and inoculation methodology for the study of soft root rot caused by Phytopythium sp.

Culture medium and inoculation methodology for the study of soft root rot caused by Phytopythium sp.

Biometry of oogonia and oospores of Saprolegniaceae

Comparison of Physical Maps of Ribosomal DNA Repeating Units in Pythium, Phytophthora and Apodachlya

Contact Info

Product

Resources

About