Long-term tracking of endoplasmic reticulum autophagy by a fluorescent probe with a long alkyl chain

Yang, Rui; Meng, Fanqing; Niu, Guangle; Niu, Jie; Hao, Qiuhua; Zong, Chong; Gao, Peng; Yu, Xiaoqiang

doi:10.1016/j.snb.2022.132639

Cited by 8 publications

(2 citation statements)

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“…8 Nutrient deprivation activates the production of misfolded proteins, which cannot be degraded by This journal is © The Royal Society of Chemistry 2023 ER-associated degradation, and thus are degraded by ER-tolysosome-associated degradation (ERLAD). 28 We have utilized the JER to monitor the ER-phagy process induced by cell starvation. For this purpose, cells were first treated with commercially available LysoTracker Green (LTG) and JER for 30 mins and then nutrient free medium was added for the mentioned period (0-3 h) and the cells were subsequently imaged under a microscope.…”

Section: Akshay Silswal and Apurba Lal Koner *mentioning

confidence: 99%

Tracking endoplasmic reticulum viscosity during ferroptosis and autophagy using a molecular rotor probe

Silswal

Koner

2023

Chem. Commun.

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Section: Akshay Silswal and Apurba Lal Koner *mentioning

confidence: 99%

Tracking endoplasmic reticulum viscosity during ferroptosis and autophagy using a molecular rotor probe

Silswal

Koner

2023

Chem. Commun.

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“…With the aid of β-CD, V-1 could target ER in HeLa cells with dual color due to the two comprising fluorophores (BODIPY/coumarin), and they used the probe to study ER membrane fluidity induced by various ER stress. To solve the permeability problems of ER probes with long alkyl chain, Yu's group developed a viscosity-sensitive probe with rotor properties for visualizing ER with high-fidelity [63]. The probe has excellent permeability, and it was used to real-time monitor ER-autophagy.…”

Section: Organic Probes For Endoplasmic Reticulum Viscositymentioning

confidence: 99%

Organic Fluorescent Probes for Monitoring Micro-Environments in Living Cells and Tissues

Yang

Zhu

et al. 2023

Molecules

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As a vital parameter in living cells and tissues, the micro-environment is crucial for the living organisms. Significantly, organelles require proper micro-environment to achieve normal physiological processes, and the micro-environment in organelles can reflect the state of organelles in living cells. Moreover, some abnormal micro-environments in organelles are closely related to organelle dysfunction and disease development. So, visualizing and monitoring the variation of micro-environments in organelles is helpful for physiologists and pathologists to study the mechanisms of the relative diseases. Recently, a large variety of fluorescent probes was developed to study the micro-environments in living cells and tissues. However, the systematic and comprehensive reviews on the organelle micro-environment in living cells and tissues have rarely been published, which may hinder the research progress in the field of organic fluorescent probes. In this review, we will summarize the organic fluorescent probes for monitoring the microenvironment, such as viscosity, pH values, polarity, and temperature. Further, diverse organelles (mitochondria, lysosome, endoplasmic reticulum, cell membrane) about microenvironments will be displayed. In this process, the fluorescent probes about the “off-on” and ratiometric category (the diverse fluorescence emission) will be discussed. Moreover, the molecular designing, chemical synthesis, fluorescent mechanism, and the bio-applications of these organic fluorescent probes in cells and tissues will also be discussed. Significantly, the merits and defects of current microenvironment-sensitive probes are outlined and discussed, and the development tendency and challenges for this kind of probe are presented. In brief, this review mainly summarizes some typical examples and highlights the progress of organic fluorescent probes for monitoring micro-environments in living cells and tissues in recent research. We anticipate that this review will deepen the understanding of microenvironment in cells and tissues and facilitate the studies and development of physiology and pathology.

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Hyperbranched polysiloxane-based probe with enhanced lipophilicity for visualizing ONOO− fluctuations in endoplasmic reticulum

Zhang

Lan

et al. 2023

Analytica Chimica Acta

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Long-term tracking of endoplasmic reticulum autophagy by a fluorescent probe with a long alkyl chain

Cited by 8 publications

References 40 publications

Tracking endoplasmic reticulum viscosity during ferroptosis and autophagy using a molecular rotor probe

Tracking endoplasmic reticulum viscosity during ferroptosis and autophagy using a molecular rotor probe

Organic Fluorescent Probes for Monitoring Micro-Environments in Living Cells and Tissues

Hyperbranched polysiloxane-based probe with enhanced lipophilicity for visualizing ONOO− fluctuations in endoplasmic reticulum

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